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Effects of inactivated Bordetella pertussis on phosphodiesterase in the lung of ovalbumin sensitized and challenged rats.

Wang YJ, Song SD, Chen JC, Wang XF, Jiang YL, Xie QM, Chen JQ, Li ZG, Tang HF - Pulm Med (2014)

Bottom Line: This paper indicated that inactivated Bordetella pertussis (iBp) can enhance the lung airway hyperreactivity of the rats sensitized and challenged with OVA.The mechanisms were involved in the upregulation of cAMP-PDE activity and PDE4A, PDE4D, and PDE3 gene expression in the lungs.So, our data suggested that cosensitization with OVA and iBp affects lung airway reactivity by modulating the lung cAMP-PDE activity and PDE4D gene expression.

View Article: PubMed Central - PubMed

Affiliation: Zhejiang Respiratory Drugs Research Laboratory of SFDA of China, School of Medicine, Zhejiang University, Hangzhou 310058, China ; Clinical College of Integrated Traditional and Western Medicine, Anhui University of Chinese Medicine, Hefei 230038, China.

ABSTRACT
This paper indicated that inactivated Bordetella pertussis (iBp) can enhance the lung airway hyperreactivity of the rats sensitized and challenged with OVA. The mechanisms were involved in the upregulation of cAMP-PDE activity and PDE4A, PDE4D, and PDE3 gene expression in the lungs. But only PDE4 activity was different between the OVA and OVA+iBp groups, and PDE4D expression was significantly increased in iBp rats alone. So, our data suggested that cosensitization with OVA and iBp affects lung airway reactivity by modulating the lung cAMP-PDE activity and PDE4D gene expression.

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Inhibitory effects (%) of theophylline (a), SKF94836 (b), zaprinast (c), and rolipram (d) on the cGMP-PDE activity in the lung homogenates harvested from OVA+iBp and OVA rats (mean ± SEM).
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fig4: Inhibitory effects (%) of theophylline (a), SKF94836 (b), zaprinast (c), and rolipram (d) on the cGMP-PDE activity in the lung homogenates harvested from OVA+iBp and OVA rats (mean ± SEM).

Mentions: To further determine which PDE subtype plays a key role in the total PDE activity, we used the theophylline (nonselective PDE inhibitor), rolipram (PDE4 inhibitor), SKF94836 (PDE3 inhibitor), and zaprinast (PDE5 inhibitor) to determine the overall PDE activity of each subtype. Rolipram (100 μM) sensitive cAMP-PDE activity (i.e., PDE4 activity) was 1.4-fold or 1.6-fold higher in the OVA or OVA+iBp rats than in normal rats (Figure 3(d)). These results suggest that the increase in total cAMP-PDE activity by OVA sensitization and challenge is primarily due to upregulation of PDE4. Zaprinast (100 μM) sensitive cGMP-PDE activity (i.e., PDE5 activity) was the major resource of cGMP-PDE activity, but the OVA or the OVA+iBp rats were similar to normal rats (Figure 4(c)).


Effects of inactivated Bordetella pertussis on phosphodiesterase in the lung of ovalbumin sensitized and challenged rats.

Wang YJ, Song SD, Chen JC, Wang XF, Jiang YL, Xie QM, Chen JQ, Li ZG, Tang HF - Pulm Med (2014)

Inhibitory effects (%) of theophylline (a), SKF94836 (b), zaprinast (c), and rolipram (d) on the cGMP-PDE activity in the lung homogenates harvested from OVA+iBp and OVA rats (mean ± SEM).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4121004&req=5

fig4: Inhibitory effects (%) of theophylline (a), SKF94836 (b), zaprinast (c), and rolipram (d) on the cGMP-PDE activity in the lung homogenates harvested from OVA+iBp and OVA rats (mean ± SEM).
Mentions: To further determine which PDE subtype plays a key role in the total PDE activity, we used the theophylline (nonselective PDE inhibitor), rolipram (PDE4 inhibitor), SKF94836 (PDE3 inhibitor), and zaprinast (PDE5 inhibitor) to determine the overall PDE activity of each subtype. Rolipram (100 μM) sensitive cAMP-PDE activity (i.e., PDE4 activity) was 1.4-fold or 1.6-fold higher in the OVA or OVA+iBp rats than in normal rats (Figure 3(d)). These results suggest that the increase in total cAMP-PDE activity by OVA sensitization and challenge is primarily due to upregulation of PDE4. Zaprinast (100 μM) sensitive cGMP-PDE activity (i.e., PDE5 activity) was the major resource of cGMP-PDE activity, but the OVA or the OVA+iBp rats were similar to normal rats (Figure 4(c)).

Bottom Line: This paper indicated that inactivated Bordetella pertussis (iBp) can enhance the lung airway hyperreactivity of the rats sensitized and challenged with OVA.The mechanisms were involved in the upregulation of cAMP-PDE activity and PDE4A, PDE4D, and PDE3 gene expression in the lungs.So, our data suggested that cosensitization with OVA and iBp affects lung airway reactivity by modulating the lung cAMP-PDE activity and PDE4D gene expression.

View Article: PubMed Central - PubMed

Affiliation: Zhejiang Respiratory Drugs Research Laboratory of SFDA of China, School of Medicine, Zhejiang University, Hangzhou 310058, China ; Clinical College of Integrated Traditional and Western Medicine, Anhui University of Chinese Medicine, Hefei 230038, China.

ABSTRACT
This paper indicated that inactivated Bordetella pertussis (iBp) can enhance the lung airway hyperreactivity of the rats sensitized and challenged with OVA. The mechanisms were involved in the upregulation of cAMP-PDE activity and PDE4A, PDE4D, and PDE3 gene expression in the lungs. But only PDE4 activity was different between the OVA and OVA+iBp groups, and PDE4D expression was significantly increased in iBp rats alone. So, our data suggested that cosensitization with OVA and iBp affects lung airway reactivity by modulating the lung cAMP-PDE activity and PDE4D gene expression.

Show MeSH
Related in: MedlinePlus