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Effects of inactivated Bordetella pertussis on phosphodiesterase in the lung of ovalbumin sensitized and challenged rats.

Wang YJ, Song SD, Chen JC, Wang XF, Jiang YL, Xie QM, Chen JQ, Li ZG, Tang HF - Pulm Med (2014)

Bottom Line: This paper indicated that inactivated Bordetella pertussis (iBp) can enhance the lung airway hyperreactivity of the rats sensitized and challenged with OVA.The mechanisms were involved in the upregulation of cAMP-PDE activity and PDE4A, PDE4D, and PDE3 gene expression in the lungs.So, our data suggested that cosensitization with OVA and iBp affects lung airway reactivity by modulating the lung cAMP-PDE activity and PDE4D gene expression.

View Article: PubMed Central - PubMed

Affiliation: Zhejiang Respiratory Drugs Research Laboratory of SFDA of China, School of Medicine, Zhejiang University, Hangzhou 310058, China ; Clinical College of Integrated Traditional and Western Medicine, Anhui University of Chinese Medicine, Hefei 230038, China.

ABSTRACT
This paper indicated that inactivated Bordetella pertussis (iBp) can enhance the lung airway hyperreactivity of the rats sensitized and challenged with OVA. The mechanisms were involved in the upregulation of cAMP-PDE activity and PDE4A, PDE4D, and PDE3 gene expression in the lungs. But only PDE4 activity was different between the OVA and OVA+iBp groups, and PDE4D expression was significantly increased in iBp rats alone. So, our data suggested that cosensitization with OVA and iBp affects lung airway reactivity by modulating the lung cAMP-PDE activity and PDE4D gene expression.

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(a) Lung cAMP-PDE activity in normal (n = 8), OVA+iBp, iBp (n = 6), and OVA rats (n = 8); (b) lung cGMP-PDE activity in normal (n = 8), OVA+iBp, iBp (n = 6), and OVA rats (n = 8) (mean ± SEM; *P < 0.05; **P < 0.01 versus OVA+iBp; #P < 0.05; ##P < 0.01 versus normal).
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fig2: (a) Lung cAMP-PDE activity in normal (n = 8), OVA+iBp, iBp (n = 6), and OVA rats (n = 8); (b) lung cGMP-PDE activity in normal (n = 8), OVA+iBp, iBp (n = 6), and OVA rats (n = 8) (mean ± SEM; *P < 0.05; **P < 0.01 versus OVA+iBp; #P < 0.05; ##P < 0.01 versus normal).

Mentions: With regard to PDE regulation in the lung, OVA sensitization and challenge with or without iBp significantly elevated the cAMP-PDE activity, resulting in 2.5-fold or 2-fold higher activity than that of normal animals, although the difference between OVA and OVA+iBp groups was not statistically significant (Figure 2(a)). But iBp did not affect the cAMP-PDE activity; the level was equivalent to the normal control. The total cGMP-PDE activity in the normal control and the OVA groups was high, while in OVA+iBp and iBp groups the activity was decreased (Figure 2(b)).


Effects of inactivated Bordetella pertussis on phosphodiesterase in the lung of ovalbumin sensitized and challenged rats.

Wang YJ, Song SD, Chen JC, Wang XF, Jiang YL, Xie QM, Chen JQ, Li ZG, Tang HF - Pulm Med (2014)

(a) Lung cAMP-PDE activity in normal (n = 8), OVA+iBp, iBp (n = 6), and OVA rats (n = 8); (b) lung cGMP-PDE activity in normal (n = 8), OVA+iBp, iBp (n = 6), and OVA rats (n = 8) (mean ± SEM; *P < 0.05; **P < 0.01 versus OVA+iBp; #P < 0.05; ##P < 0.01 versus normal).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4121004&req=5

fig2: (a) Lung cAMP-PDE activity in normal (n = 8), OVA+iBp, iBp (n = 6), and OVA rats (n = 8); (b) lung cGMP-PDE activity in normal (n = 8), OVA+iBp, iBp (n = 6), and OVA rats (n = 8) (mean ± SEM; *P < 0.05; **P < 0.01 versus OVA+iBp; #P < 0.05; ##P < 0.01 versus normal).
Mentions: With regard to PDE regulation in the lung, OVA sensitization and challenge with or without iBp significantly elevated the cAMP-PDE activity, resulting in 2.5-fold or 2-fold higher activity than that of normal animals, although the difference between OVA and OVA+iBp groups was not statistically significant (Figure 2(a)). But iBp did not affect the cAMP-PDE activity; the level was equivalent to the normal control. The total cGMP-PDE activity in the normal control and the OVA groups was high, while in OVA+iBp and iBp groups the activity was decreased (Figure 2(b)).

Bottom Line: This paper indicated that inactivated Bordetella pertussis (iBp) can enhance the lung airway hyperreactivity of the rats sensitized and challenged with OVA.The mechanisms were involved in the upregulation of cAMP-PDE activity and PDE4A, PDE4D, and PDE3 gene expression in the lungs.So, our data suggested that cosensitization with OVA and iBp affects lung airway reactivity by modulating the lung cAMP-PDE activity and PDE4D gene expression.

View Article: PubMed Central - PubMed

Affiliation: Zhejiang Respiratory Drugs Research Laboratory of SFDA of China, School of Medicine, Zhejiang University, Hangzhou 310058, China ; Clinical College of Integrated Traditional and Western Medicine, Anhui University of Chinese Medicine, Hefei 230038, China.

ABSTRACT
This paper indicated that inactivated Bordetella pertussis (iBp) can enhance the lung airway hyperreactivity of the rats sensitized and challenged with OVA. The mechanisms were involved in the upregulation of cAMP-PDE activity and PDE4A, PDE4D, and PDE3 gene expression in the lungs. But only PDE4 activity was different between the OVA and OVA+iBp groups, and PDE4D expression was significantly increased in iBp rats alone. So, our data suggested that cosensitization with OVA and iBp affects lung airway reactivity by modulating the lung cAMP-PDE activity and PDE4D gene expression.

Show MeSH
Related in: MedlinePlus