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Lipid isolated from a Leishmania donovani strain reduces Escherichia coli induced sepsis in mice through inhibition of inflammatory responses.

Das S, Chatterjee N, Bose D, Banerjee S, Pal P, Jha T, Das Saha K - Mediators Inflamm. (2014)

Bottom Line: Due to the effect of LTL, E. coli induced inflammatory cytokine-chemokine(s) levels were significantly reduced in serum and bronchoalveolar lavage fluid simultaneously.LTL also improved the lung injury and suppressed the cell adhesion molecules in lung tissue.These findings indicate that LTL may prove to be a potential anti-inflammatory agent and provide protection against gram-negative bacterial sepsis with pulmonary impairment.

View Article: PubMed Central - PubMed

Affiliation: Cancer Biology & Inflammatory Disorder Division, CSIR-Indian Institute of Chemical Biology, 4 Raja S.C. Mullick Road, Kolkata, West Bengal 700032, India.

ABSTRACT
Sepsis is the reflection of systemic immune response that manifests in the sequential inflammatory process in presence of infection. This may occur as a result of gram-negative bacterial sepsis including Escherichia coli infection that gives rise to excessive production of inflammatory mediators and causes severe tissue injuries. We have reported earlier that the lipid of attenuated Leishmania donovani suppresses the inflammatory responses in arthritis patients. Using heat killed E. coli stimulated macrophages, we have now investigated the effect of leishmanial total lipid (LTL) isolated from Leishmania donovani (MHO/IN/1978/UR6) for amelioration of the inflammatory mediators and transcriptional factor with suppression of TLR4-CD14 expression. To evaluate the in vivo effect, E. coli induced murine sepsis model was used focusing on the changes in different parameter(s) of lung injury caused by sepsis, namely, edema, vascular permeability, and pathophysiology, and the status of different cytokine-chemokine(s) and adhesion molecule(s). Due to the effect of LTL, E. coli induced inflammatory cytokine-chemokine(s) levels were significantly reduced in serum and bronchoalveolar lavage fluid simultaneously. LTL also improved the lung injury and suppressed the cell adhesion molecules in lung tissue. These findings indicate that LTL may prove to be a potential anti-inflammatory agent and provide protection against gram-negative bacterial sepsis with pulmonary impairment.

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Related in: MedlinePlus

Thin-layer chromatography (TLC) profile of leishmanial total lipid or LTL (a). Effect of LTL on production of PGE2 (b) and TNF-α (c). The mouse peritoneal macrophage cells were preincubated with LTL in the presence or absence of heat-killed E. coli (O18:K1; 1 × 108 CFU/mL) for 24 h and the optical density was determined by ELISA method. (d) The cytotoxicity of LTL on peritoneal macrophage cell measured by MTT assay for 24 h and OD determination at 595 nm. The data are reported as the mean ± SEM of triplicate experiments. (*P < 0.05, **P < 0.01).
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fig1: Thin-layer chromatography (TLC) profile of leishmanial total lipid or LTL (a). Effect of LTL on production of PGE2 (b) and TNF-α (c). The mouse peritoneal macrophage cells were preincubated with LTL in the presence or absence of heat-killed E. coli (O18:K1; 1 × 108 CFU/mL) for 24 h and the optical density was determined by ELISA method. (d) The cytotoxicity of LTL on peritoneal macrophage cell measured by MTT assay for 24 h and OD determination at 595 nm. The data are reported as the mean ± SEM of triplicate experiments. (*P < 0.05, **P < 0.01).

Mentions: Iodine staining showed six spots of lipids in the TLC plate. Lipids from three different batches showing the same TLC profile (Figure 1(a)) were used in further studies.


Lipid isolated from a Leishmania donovani strain reduces Escherichia coli induced sepsis in mice through inhibition of inflammatory responses.

Das S, Chatterjee N, Bose D, Banerjee S, Pal P, Jha T, Das Saha K - Mediators Inflamm. (2014)

Thin-layer chromatography (TLC) profile of leishmanial total lipid or LTL (a). Effect of LTL on production of PGE2 (b) and TNF-α (c). The mouse peritoneal macrophage cells were preincubated with LTL in the presence or absence of heat-killed E. coli (O18:K1; 1 × 108 CFU/mL) for 24 h and the optical density was determined by ELISA method. (d) The cytotoxicity of LTL on peritoneal macrophage cell measured by MTT assay for 24 h and OD determination at 595 nm. The data are reported as the mean ± SEM of triplicate experiments. (*P < 0.05, **P < 0.01).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4120923&req=5

fig1: Thin-layer chromatography (TLC) profile of leishmanial total lipid or LTL (a). Effect of LTL on production of PGE2 (b) and TNF-α (c). The mouse peritoneal macrophage cells were preincubated with LTL in the presence or absence of heat-killed E. coli (O18:K1; 1 × 108 CFU/mL) for 24 h and the optical density was determined by ELISA method. (d) The cytotoxicity of LTL on peritoneal macrophage cell measured by MTT assay for 24 h and OD determination at 595 nm. The data are reported as the mean ± SEM of triplicate experiments. (*P < 0.05, **P < 0.01).
Mentions: Iodine staining showed six spots of lipids in the TLC plate. Lipids from three different batches showing the same TLC profile (Figure 1(a)) were used in further studies.

Bottom Line: Due to the effect of LTL, E. coli induced inflammatory cytokine-chemokine(s) levels were significantly reduced in serum and bronchoalveolar lavage fluid simultaneously.LTL also improved the lung injury and suppressed the cell adhesion molecules in lung tissue.These findings indicate that LTL may prove to be a potential anti-inflammatory agent and provide protection against gram-negative bacterial sepsis with pulmonary impairment.

View Article: PubMed Central - PubMed

Affiliation: Cancer Biology & Inflammatory Disorder Division, CSIR-Indian Institute of Chemical Biology, 4 Raja S.C. Mullick Road, Kolkata, West Bengal 700032, India.

ABSTRACT
Sepsis is the reflection of systemic immune response that manifests in the sequential inflammatory process in presence of infection. This may occur as a result of gram-negative bacterial sepsis including Escherichia coli infection that gives rise to excessive production of inflammatory mediators and causes severe tissue injuries. We have reported earlier that the lipid of attenuated Leishmania donovani suppresses the inflammatory responses in arthritis patients. Using heat killed E. coli stimulated macrophages, we have now investigated the effect of leishmanial total lipid (LTL) isolated from Leishmania donovani (MHO/IN/1978/UR6) for amelioration of the inflammatory mediators and transcriptional factor with suppression of TLR4-CD14 expression. To evaluate the in vivo effect, E. coli induced murine sepsis model was used focusing on the changes in different parameter(s) of lung injury caused by sepsis, namely, edema, vascular permeability, and pathophysiology, and the status of different cytokine-chemokine(s) and adhesion molecule(s). Due to the effect of LTL, E. coli induced inflammatory cytokine-chemokine(s) levels were significantly reduced in serum and bronchoalveolar lavage fluid simultaneously. LTL also improved the lung injury and suppressed the cell adhesion molecules in lung tissue. These findings indicate that LTL may prove to be a potential anti-inflammatory agent and provide protection against gram-negative bacterial sepsis with pulmonary impairment.

Show MeSH
Related in: MedlinePlus