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Combination of acellular nerve graft and schwann cells-like cells for rat sciatic nerve regeneration.

Gao S, Zheng Y, Cai Q, Deng Z, Yao W, Wang J, Wang X, Zhang P - Neural Plast. (2014)

Bottom Line: To investigate the effect of tissue engineering nerve on repair of rat sciatic nerve defect.After six and twelve weeks, the recovery ratio of SFI and wet weight of gastrocnemius muscle, NEP, and the result of regenerated myelinated nerve fibers in groups B and C were superior to that of group A (P < 0.05), and the difference between groups B and C was not statistically significant (P > 0.05).The tissue engineering nerve composed of acellular allogenic nerve scaffold and Schwann cells-like cells can effectively repair the nerve defect in rats and its effect was similar to that of the autogenous nerve grafts.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics, The Affiliated Tumor Hospital of Zhengzhou University, No. 127, Dongming Road, Zhengzhou, Henan 450008, China.

ABSTRACT

Objective: To investigate the effect of tissue engineering nerve on repair of rat sciatic nerve defect.

Methods: Forty-five rats with defective sciatic nerve were randomly divided into three groups. Rats in group A were repaired by acellular nerve grafts only. Rats in group B were repaired by tissue engineering nerve. In group C, rats were repaired by autogenous nerve grafts. After six and twelve weeks, sciatic nerve functional index (SFI), neural electrophysiology (NEP), histological and transmission electron microscope observation, recovery ratio of wet weight of gastrocnemius muscle, regenerated myelinated nerve fibers number, nerve fiber diameter, and thickness of the myelin sheath were measured to assess the effect.

Results: After six and twelve weeks, the recovery ratio of SFI and wet weight of gastrocnemius muscle, NEP, and the result of regenerated myelinated nerve fibers in groups B and C were superior to that of group A (P < 0.05), and the difference between groups B and C was not statistically significant (P > 0.05).

Conclusion: The tissue engineering nerve composed of acellular allogenic nerve scaffold and Schwann cells-like cells can effectively repair the nerve defect in rats and its effect was similar to that of the autogenous nerve grafts.

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Related in: MedlinePlus

((a), (b), and (c)) HE staining of transverse nerve slices from each group at 6 w. The regenerated myelinated nerve fibers were sparse; distribution and diameter of the fibers were irregular, but there were no obvious lymphocytic infiltrates. ((d), (e), and (f)) HE staining of transverse nerve slices from each group at 12 w. (d) The number of regenerated myelinated nerve fibers in group A was increased; myelin was relatively dense and irregularly arranged. The number of regenerated myelinated nerve fibers in groups B (e) and C (f) was increased; myelin was dense and regularly arranged; there were blood capillary hyperplasia and no obvious lymphocytic infiltrates. ((g), (h), and (i)) Toluidine blue staining slices from each group at 6 w. (g) The myelin of regenerated myelinated nerve fibers in group A was sparse; the morphology and diameter were irregular. The myelin of regenerated myelinated nerve fibers in groups B (h) and C (i) was dense, but the morphology and diameter were irregular. ((j), (k), and (l)) Toluidine blue staining slices from each group at 12 w. (j) There were large numbers of regenerative and partly myelinated axon in group A and the diameter of them was homogeneous. There were large numbers of regenerative and partly myelinated axon in groups B (k) and C (l) and the diameter of them was homogeneous and myelin sheath was more thicker than group A. ((m), (n), and (o)) Transmission electron microscope from each group at 6 w. (m) The arrangement and thickness of regenerated myelin sheath in group A were irregular. (n) The arrangement of regenerated myelin sheath in group B was more regular than group A, but the thickness of myelin sheath was nonuniform. (o) The arrangement of regenerated myelin sheath in group C was more regular than group A and the thickness of myelin sheath was uniform. ((p), (q), and (r)) Transmission electron microscope from each group at 12 w. (p) The regenerated myelin sheath was thin and irregular, accompanied with Schwann cell proliferation. (q) In group B, the regenerated myelin sheath arranges compactly and thicker, accompanied with Schwann cell proliferation. (r) In group C, the regenerated myelin sheath arranges compactly and thicker than groups A and B, accompanied with Schwann cell proliferation and chondriosome, microtubule, and microfilament inside.
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fig4: ((a), (b), and (c)) HE staining of transverse nerve slices from each group at 6 w. The regenerated myelinated nerve fibers were sparse; distribution and diameter of the fibers were irregular, but there were no obvious lymphocytic infiltrates. ((d), (e), and (f)) HE staining of transverse nerve slices from each group at 12 w. (d) The number of regenerated myelinated nerve fibers in group A was increased; myelin was relatively dense and irregularly arranged. The number of regenerated myelinated nerve fibers in groups B (e) and C (f) was increased; myelin was dense and regularly arranged; there were blood capillary hyperplasia and no obvious lymphocytic infiltrates. ((g), (h), and (i)) Toluidine blue staining slices from each group at 6 w. (g) The myelin of regenerated myelinated nerve fibers in group A was sparse; the morphology and diameter were irregular. The myelin of regenerated myelinated nerve fibers in groups B (h) and C (i) was dense, but the morphology and diameter were irregular. ((j), (k), and (l)) Toluidine blue staining slices from each group at 12 w. (j) There were large numbers of regenerative and partly myelinated axon in group A and the diameter of them was homogeneous. There were large numbers of regenerative and partly myelinated axon in groups B (k) and C (l) and the diameter of them was homogeneous and myelin sheath was more thicker than group A. ((m), (n), and (o)) Transmission electron microscope from each group at 6 w. (m) The arrangement and thickness of regenerated myelin sheath in group A were irregular. (n) The arrangement of regenerated myelin sheath in group B was more regular than group A, but the thickness of myelin sheath was nonuniform. (o) The arrangement of regenerated myelin sheath in group C was more regular than group A and the thickness of myelin sheath was uniform. ((p), (q), and (r)) Transmission electron microscope from each group at 12 w. (p) The regenerated myelin sheath was thin and irregular, accompanied with Schwann cell proliferation. (q) In group B, the regenerated myelin sheath arranges compactly and thicker, accompanied with Schwann cell proliferation. (r) In group C, the regenerated myelin sheath arranges compactly and thicker than groups A and B, accompanied with Schwann cell proliferation and chondriosome, microtubule, and microfilament inside.

Mentions: 6 w following the surgery, in HE staining slices of three groups, the regenerated myelinated nerve fibers were sparse; the distribution and diameter of the fibers were irregular, but there were no obvious lymphocyte infiltration (Figures 4(a), 4(b), and 4(c)); 12 w following the surgery, in HE staining slices of three groups, the number of regenerated myelinated nerve fibers in group A was increased; although the myelin was relatively dense, it was irregularly arranged; the number of regenerated myelinated nerve fibers in groups B and C was increased, the myelin was densely and regularly arranged, and there was blood capillary hyperplasia and no obvious lymphocyte infiltration (Figures 4(d), 4(e), and 4(f)).


Combination of acellular nerve graft and schwann cells-like cells for rat sciatic nerve regeneration.

Gao S, Zheng Y, Cai Q, Deng Z, Yao W, Wang J, Wang X, Zhang P - Neural Plast. (2014)

((a), (b), and (c)) HE staining of transverse nerve slices from each group at 6 w. The regenerated myelinated nerve fibers were sparse; distribution and diameter of the fibers were irregular, but there were no obvious lymphocytic infiltrates. ((d), (e), and (f)) HE staining of transverse nerve slices from each group at 12 w. (d) The number of regenerated myelinated nerve fibers in group A was increased; myelin was relatively dense and irregularly arranged. The number of regenerated myelinated nerve fibers in groups B (e) and C (f) was increased; myelin was dense and regularly arranged; there were blood capillary hyperplasia and no obvious lymphocytic infiltrates. ((g), (h), and (i)) Toluidine blue staining slices from each group at 6 w. (g) The myelin of regenerated myelinated nerve fibers in group A was sparse; the morphology and diameter were irregular. The myelin of regenerated myelinated nerve fibers in groups B (h) and C (i) was dense, but the morphology and diameter were irregular. ((j), (k), and (l)) Toluidine blue staining slices from each group at 12 w. (j) There were large numbers of regenerative and partly myelinated axon in group A and the diameter of them was homogeneous. There were large numbers of regenerative and partly myelinated axon in groups B (k) and C (l) and the diameter of them was homogeneous and myelin sheath was more thicker than group A. ((m), (n), and (o)) Transmission electron microscope from each group at 6 w. (m) The arrangement and thickness of regenerated myelin sheath in group A were irregular. (n) The arrangement of regenerated myelin sheath in group B was more regular than group A, but the thickness of myelin sheath was nonuniform. (o) The arrangement of regenerated myelin sheath in group C was more regular than group A and the thickness of myelin sheath was uniform. ((p), (q), and (r)) Transmission electron microscope from each group at 12 w. (p) The regenerated myelin sheath was thin and irregular, accompanied with Schwann cell proliferation. (q) In group B, the regenerated myelin sheath arranges compactly and thicker, accompanied with Schwann cell proliferation. (r) In group C, the regenerated myelin sheath arranges compactly and thicker than groups A and B, accompanied with Schwann cell proliferation and chondriosome, microtubule, and microfilament inside.
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fig4: ((a), (b), and (c)) HE staining of transverse nerve slices from each group at 6 w. The regenerated myelinated nerve fibers were sparse; distribution and diameter of the fibers were irregular, but there were no obvious lymphocytic infiltrates. ((d), (e), and (f)) HE staining of transverse nerve slices from each group at 12 w. (d) The number of regenerated myelinated nerve fibers in group A was increased; myelin was relatively dense and irregularly arranged. The number of regenerated myelinated nerve fibers in groups B (e) and C (f) was increased; myelin was dense and regularly arranged; there were blood capillary hyperplasia and no obvious lymphocytic infiltrates. ((g), (h), and (i)) Toluidine blue staining slices from each group at 6 w. (g) The myelin of regenerated myelinated nerve fibers in group A was sparse; the morphology and diameter were irregular. The myelin of regenerated myelinated nerve fibers in groups B (h) and C (i) was dense, but the morphology and diameter were irregular. ((j), (k), and (l)) Toluidine blue staining slices from each group at 12 w. (j) There were large numbers of regenerative and partly myelinated axon in group A and the diameter of them was homogeneous. There were large numbers of regenerative and partly myelinated axon in groups B (k) and C (l) and the diameter of them was homogeneous and myelin sheath was more thicker than group A. ((m), (n), and (o)) Transmission electron microscope from each group at 6 w. (m) The arrangement and thickness of regenerated myelin sheath in group A were irregular. (n) The arrangement of regenerated myelin sheath in group B was more regular than group A, but the thickness of myelin sheath was nonuniform. (o) The arrangement of regenerated myelin sheath in group C was more regular than group A and the thickness of myelin sheath was uniform. ((p), (q), and (r)) Transmission electron microscope from each group at 12 w. (p) The regenerated myelin sheath was thin and irregular, accompanied with Schwann cell proliferation. (q) In group B, the regenerated myelin sheath arranges compactly and thicker, accompanied with Schwann cell proliferation. (r) In group C, the regenerated myelin sheath arranges compactly and thicker than groups A and B, accompanied with Schwann cell proliferation and chondriosome, microtubule, and microfilament inside.
Mentions: 6 w following the surgery, in HE staining slices of three groups, the regenerated myelinated nerve fibers were sparse; the distribution and diameter of the fibers were irregular, but there were no obvious lymphocyte infiltration (Figures 4(a), 4(b), and 4(c)); 12 w following the surgery, in HE staining slices of three groups, the number of regenerated myelinated nerve fibers in group A was increased; although the myelin was relatively dense, it was irregularly arranged; the number of regenerated myelinated nerve fibers in groups B and C was increased, the myelin was densely and regularly arranged, and there was blood capillary hyperplasia and no obvious lymphocyte infiltration (Figures 4(d), 4(e), and 4(f)).

Bottom Line: To investigate the effect of tissue engineering nerve on repair of rat sciatic nerve defect.After six and twelve weeks, the recovery ratio of SFI and wet weight of gastrocnemius muscle, NEP, and the result of regenerated myelinated nerve fibers in groups B and C were superior to that of group A (P < 0.05), and the difference between groups B and C was not statistically significant (P > 0.05).The tissue engineering nerve composed of acellular allogenic nerve scaffold and Schwann cells-like cells can effectively repair the nerve defect in rats and its effect was similar to that of the autogenous nerve grafts.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics, The Affiliated Tumor Hospital of Zhengzhou University, No. 127, Dongming Road, Zhengzhou, Henan 450008, China.

ABSTRACT

Objective: To investigate the effect of tissue engineering nerve on repair of rat sciatic nerve defect.

Methods: Forty-five rats with defective sciatic nerve were randomly divided into three groups. Rats in group A were repaired by acellular nerve grafts only. Rats in group B were repaired by tissue engineering nerve. In group C, rats were repaired by autogenous nerve grafts. After six and twelve weeks, sciatic nerve functional index (SFI), neural electrophysiology (NEP), histological and transmission electron microscope observation, recovery ratio of wet weight of gastrocnemius muscle, regenerated myelinated nerve fibers number, nerve fiber diameter, and thickness of the myelin sheath were measured to assess the effect.

Results: After six and twelve weeks, the recovery ratio of SFI and wet weight of gastrocnemius muscle, NEP, and the result of regenerated myelinated nerve fibers in groups B and C were superior to that of group A (P < 0.05), and the difference between groups B and C was not statistically significant (P > 0.05).

Conclusion: The tissue engineering nerve composed of acellular allogenic nerve scaffold and Schwann cells-like cells can effectively repair the nerve defect in rats and its effect was similar to that of the autogenous nerve grafts.

Show MeSH
Related in: MedlinePlus