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Combination of acellular nerve graft and schwann cells-like cells for rat sciatic nerve regeneration.

Gao S, Zheng Y, Cai Q, Deng Z, Yao W, Wang J, Wang X, Zhang P - Neural Plast. (2014)

Bottom Line: To investigate the effect of tissue engineering nerve on repair of rat sciatic nerve defect.After six and twelve weeks, the recovery ratio of SFI and wet weight of gastrocnemius muscle, NEP, and the result of regenerated myelinated nerve fibers in groups B and C were superior to that of group A (P < 0.05), and the difference between groups B and C was not statistically significant (P > 0.05).The tissue engineering nerve composed of acellular allogenic nerve scaffold and Schwann cells-like cells can effectively repair the nerve defect in rats and its effect was similar to that of the autogenous nerve grafts.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics, The Affiliated Tumor Hospital of Zhengzhou University, No. 127, Dongming Road, Zhengzhou, Henan 450008, China.

ABSTRACT

Objective: To investigate the effect of tissue engineering nerve on repair of rat sciatic nerve defect.

Methods: Forty-five rats with defective sciatic nerve were randomly divided into three groups. Rats in group A were repaired by acellular nerve grafts only. Rats in group B were repaired by tissue engineering nerve. In group C, rats were repaired by autogenous nerve grafts. After six and twelve weeks, sciatic nerve functional index (SFI), neural electrophysiology (NEP), histological and transmission electron microscope observation, recovery ratio of wet weight of gastrocnemius muscle, regenerated myelinated nerve fibers number, nerve fiber diameter, and thickness of the myelin sheath were measured to assess the effect.

Results: After six and twelve weeks, the recovery ratio of SFI and wet weight of gastrocnemius muscle, NEP, and the result of regenerated myelinated nerve fibers in groups B and C were superior to that of group A (P < 0.05), and the difference between groups B and C was not statistically significant (P > 0.05).

Conclusion: The tissue engineering nerve composed of acellular allogenic nerve scaffold and Schwann cells-like cells can effectively repair the nerve defect in rats and its effect was similar to that of the autogenous nerve grafts.

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Related in: MedlinePlus

((a), (b), and (c)) Neural electrophysiology of group A (a), group B (b), and group C (c) at 6 weeks. ((d), (e), and (f)) Neural electrophysiology of group A (d), group B (e), and group C (f) at 12 weeks.
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fig3: ((a), (b), and (c)) Neural electrophysiology of group A (a), group B (b), and group C (c) at 6 weeks. ((d), (e), and (f)) Neural electrophysiology of group A (d), group B (e), and group C (f) at 12 weeks.

Mentions: 6 w and 12 w following the surgery, the recovery ratio of MNCV and CMAP of groups B and C was superior to that of group A (P < 0.05), but the difference between groups B and C was not statistically significant (P > 0.05) (Table 1, Figure 3).


Combination of acellular nerve graft and schwann cells-like cells for rat sciatic nerve regeneration.

Gao S, Zheng Y, Cai Q, Deng Z, Yao W, Wang J, Wang X, Zhang P - Neural Plast. (2014)

((a), (b), and (c)) Neural electrophysiology of group A (a), group B (b), and group C (c) at 6 weeks. ((d), (e), and (f)) Neural electrophysiology of group A (d), group B (e), and group C (f) at 12 weeks.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4120921&req=5

fig3: ((a), (b), and (c)) Neural electrophysiology of group A (a), group B (b), and group C (c) at 6 weeks. ((d), (e), and (f)) Neural electrophysiology of group A (d), group B (e), and group C (f) at 12 weeks.
Mentions: 6 w and 12 w following the surgery, the recovery ratio of MNCV and CMAP of groups B and C was superior to that of group A (P < 0.05), but the difference between groups B and C was not statistically significant (P > 0.05) (Table 1, Figure 3).

Bottom Line: To investigate the effect of tissue engineering nerve on repair of rat sciatic nerve defect.After six and twelve weeks, the recovery ratio of SFI and wet weight of gastrocnemius muscle, NEP, and the result of regenerated myelinated nerve fibers in groups B and C were superior to that of group A (P < 0.05), and the difference between groups B and C was not statistically significant (P > 0.05).The tissue engineering nerve composed of acellular allogenic nerve scaffold and Schwann cells-like cells can effectively repair the nerve defect in rats and its effect was similar to that of the autogenous nerve grafts.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics, The Affiliated Tumor Hospital of Zhengzhou University, No. 127, Dongming Road, Zhengzhou, Henan 450008, China.

ABSTRACT

Objective: To investigate the effect of tissue engineering nerve on repair of rat sciatic nerve defect.

Methods: Forty-five rats with defective sciatic nerve were randomly divided into three groups. Rats in group A were repaired by acellular nerve grafts only. Rats in group B were repaired by tissue engineering nerve. In group C, rats were repaired by autogenous nerve grafts. After six and twelve weeks, sciatic nerve functional index (SFI), neural electrophysiology (NEP), histological and transmission electron microscope observation, recovery ratio of wet weight of gastrocnemius muscle, regenerated myelinated nerve fibers number, nerve fiber diameter, and thickness of the myelin sheath were measured to assess the effect.

Results: After six and twelve weeks, the recovery ratio of SFI and wet weight of gastrocnemius muscle, NEP, and the result of regenerated myelinated nerve fibers in groups B and C were superior to that of group A (P < 0.05), and the difference between groups B and C was not statistically significant (P > 0.05).

Conclusion: The tissue engineering nerve composed of acellular allogenic nerve scaffold and Schwann cells-like cells can effectively repair the nerve defect in rats and its effect was similar to that of the autogenous nerve grafts.

Show MeSH
Related in: MedlinePlus