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Combination of acellular nerve graft and schwann cells-like cells for rat sciatic nerve regeneration.

Gao S, Zheng Y, Cai Q, Deng Z, Yao W, Wang J, Wang X, Zhang P - Neural Plast. (2014)

Bottom Line: To investigate the effect of tissue engineering nerve on repair of rat sciatic nerve defect.After six and twelve weeks, the recovery ratio of SFI and wet weight of gastrocnemius muscle, NEP, and the result of regenerated myelinated nerve fibers in groups B and C were superior to that of group A (P < 0.05), and the difference between groups B and C was not statistically significant (P > 0.05).The tissue engineering nerve composed of acellular allogenic nerve scaffold and Schwann cells-like cells can effectively repair the nerve defect in rats and its effect was similar to that of the autogenous nerve grafts.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics, The Affiliated Tumor Hospital of Zhengzhou University, No. 127, Dongming Road, Zhengzhou, Henan 450008, China.

ABSTRACT

Objective: To investigate the effect of tissue engineering nerve on repair of rat sciatic nerve defect.

Methods: Forty-five rats with defective sciatic nerve were randomly divided into three groups. Rats in group A were repaired by acellular nerve grafts only. Rats in group B were repaired by tissue engineering nerve. In group C, rats were repaired by autogenous nerve grafts. After six and twelve weeks, sciatic nerve functional index (SFI), neural electrophysiology (NEP), histological and transmission electron microscope observation, recovery ratio of wet weight of gastrocnemius muscle, regenerated myelinated nerve fibers number, nerve fiber diameter, and thickness of the myelin sheath were measured to assess the effect.

Results: After six and twelve weeks, the recovery ratio of SFI and wet weight of gastrocnemius muscle, NEP, and the result of regenerated myelinated nerve fibers in groups B and C were superior to that of group A (P < 0.05), and the difference between groups B and C was not statistically significant (P > 0.05).

Conclusion: The tissue engineering nerve composed of acellular allogenic nerve scaffold and Schwann cells-like cells can effectively repair the nerve defect in rats and its effect was similar to that of the autogenous nerve grafts.

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Related in: MedlinePlus

(a) Immunocytochemical staining of GFAP in Schwann cells-like cells that differentiated from ADSCs. (b) Immunocytochemical staining of S-100 in Schwann cells-like cells that differentiated from ADSCs. (c) BrdU and S-100 staining of longitudinal nerve slices from group B at 6 w, blue BrdU-labeled Schwann cells-like cells' nucleus, and brown S100-labeled staining myelin sheath were observed under microscope. (d) BrdU and S-100 staining of longitudinal nerve slices from group B at 12 w, blue BrdU-labeled Schwann cells-like cells' nucleus, and brown S100-labeled staining myelin sheath were observed under microscope.
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fig1: (a) Immunocytochemical staining of GFAP in Schwann cells-like cells that differentiated from ADSCs. (b) Immunocytochemical staining of S-100 in Schwann cells-like cells that differentiated from ADSCs. (c) BrdU and S-100 staining of longitudinal nerve slices from group B at 6 w, blue BrdU-labeled Schwann cells-like cells' nucleus, and brown S100-labeled staining myelin sheath were observed under microscope. (d) BrdU and S-100 staining of longitudinal nerve slices from group B at 12 w, blue BrdU-labeled Schwann cells-like cells' nucleus, and brown S100-labeled staining myelin sheath were observed under microscope.

Mentions: After staining by S-100 and GFAP, the cytoplasm of the positive staining cells was yellow dyed; the morphology of positive staining cells was consistent with that of living cells observed under inverted microscope (Figures 1(a) and 1(b)).


Combination of acellular nerve graft and schwann cells-like cells for rat sciatic nerve regeneration.

Gao S, Zheng Y, Cai Q, Deng Z, Yao W, Wang J, Wang X, Zhang P - Neural Plast. (2014)

(a) Immunocytochemical staining of GFAP in Schwann cells-like cells that differentiated from ADSCs. (b) Immunocytochemical staining of S-100 in Schwann cells-like cells that differentiated from ADSCs. (c) BrdU and S-100 staining of longitudinal nerve slices from group B at 6 w, blue BrdU-labeled Schwann cells-like cells' nucleus, and brown S100-labeled staining myelin sheath were observed under microscope. (d) BrdU and S-100 staining of longitudinal nerve slices from group B at 12 w, blue BrdU-labeled Schwann cells-like cells' nucleus, and brown S100-labeled staining myelin sheath were observed under microscope.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4120921&req=5

fig1: (a) Immunocytochemical staining of GFAP in Schwann cells-like cells that differentiated from ADSCs. (b) Immunocytochemical staining of S-100 in Schwann cells-like cells that differentiated from ADSCs. (c) BrdU and S-100 staining of longitudinal nerve slices from group B at 6 w, blue BrdU-labeled Schwann cells-like cells' nucleus, and brown S100-labeled staining myelin sheath were observed under microscope. (d) BrdU and S-100 staining of longitudinal nerve slices from group B at 12 w, blue BrdU-labeled Schwann cells-like cells' nucleus, and brown S100-labeled staining myelin sheath were observed under microscope.
Mentions: After staining by S-100 and GFAP, the cytoplasm of the positive staining cells was yellow dyed; the morphology of positive staining cells was consistent with that of living cells observed under inverted microscope (Figures 1(a) and 1(b)).

Bottom Line: To investigate the effect of tissue engineering nerve on repair of rat sciatic nerve defect.After six and twelve weeks, the recovery ratio of SFI and wet weight of gastrocnemius muscle, NEP, and the result of regenerated myelinated nerve fibers in groups B and C were superior to that of group A (P < 0.05), and the difference between groups B and C was not statistically significant (P > 0.05).The tissue engineering nerve composed of acellular allogenic nerve scaffold and Schwann cells-like cells can effectively repair the nerve defect in rats and its effect was similar to that of the autogenous nerve grafts.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics, The Affiliated Tumor Hospital of Zhengzhou University, No. 127, Dongming Road, Zhengzhou, Henan 450008, China.

ABSTRACT

Objective: To investigate the effect of tissue engineering nerve on repair of rat sciatic nerve defect.

Methods: Forty-five rats with defective sciatic nerve were randomly divided into three groups. Rats in group A were repaired by acellular nerve grafts only. Rats in group B were repaired by tissue engineering nerve. In group C, rats were repaired by autogenous nerve grafts. After six and twelve weeks, sciatic nerve functional index (SFI), neural electrophysiology (NEP), histological and transmission electron microscope observation, recovery ratio of wet weight of gastrocnemius muscle, regenerated myelinated nerve fibers number, nerve fiber diameter, and thickness of the myelin sheath were measured to assess the effect.

Results: After six and twelve weeks, the recovery ratio of SFI and wet weight of gastrocnemius muscle, NEP, and the result of regenerated myelinated nerve fibers in groups B and C were superior to that of group A (P < 0.05), and the difference between groups B and C was not statistically significant (P > 0.05).

Conclusion: The tissue engineering nerve composed of acellular allogenic nerve scaffold and Schwann cells-like cells can effectively repair the nerve defect in rats and its effect was similar to that of the autogenous nerve grafts.

Show MeSH
Related in: MedlinePlus