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Exopolysaccharide from Ganoderma applanatum as a promising bioactive compound with cytostatic and antibacterial properties.

Osińska-Jaroszuk M, Jaszek M, Mizerska-Dudka M, Błachowicz A, Rejczak TP, Janusz G, Wydrych J, Polak J, Jarosz-Wilkołazka A, Kandefer-Szerszeń M - Biomed Res Int (2014)

Bottom Line: A new exopolysaccharide preparation isolated from stationary cultures of the white rot fungus Ganoderma applanatum (GpEPS) was tested in terms of its bioactive properties including its cytotoxic and immunostimulatory effect.The GpEPS showed antibacterial properties against Staphyloccoccus aureus and a toxic effect against Vibrio fischeri cells (82.8% cell damage).High cholesterol-binding capacity and triglycerides-binding capacity (57.9% and 41.6% after 24 h of incubation with the tested substances, resp.) were also detected for the investigated samples of GpEPS.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Maria Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland.

ABSTRACT
A new exopolysaccharide preparation isolated from stationary cultures of the white rot fungus Ganoderma applanatum (GpEPS) was tested in terms of its bioactive properties including its cytotoxic and immunostimulatory effect. The results indicate that the tested GpEPS (at concentrations above 22.85 µg/mL and 228.5 µg/mL) may exhibit selective activity against tumor cells (cell lines SiHa) and stimulate production of TNF-α THP-1-derived macrophages at the level of 752.17 pg/mL. The GpEPS showed antibacterial properties against Staphyloccoccus aureus and a toxic effect against Vibrio fischeri cells (82.8% cell damage). High cholesterol-binding capacity and triglycerides-binding capacity (57.9% and 41.6% after 24 h of incubation with the tested substances, resp.) were also detected for the investigated samples of GpEPS.

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Related in: MedlinePlus

Testing the ability of exopolysaccharides from G. applanatum (GpEPS)to binding: cholesterol, triglycerides, glucose, (a) and magnesium and iron ions (b) expressed as a percentage of the test substance bound to exopolysaccharides. All results are expressed as mean ± SD from three experiments (n = 3); values marked with the different letters are significantly different (P ≤ 0.05).
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fig7: Testing the ability of exopolysaccharides from G. applanatum (GpEPS)to binding: cholesterol, triglycerides, glucose, (a) and magnesium and iron ions (b) expressed as a percentage of the test substance bound to exopolysaccharides. All results are expressed as mean ± SD from three experiments (n = 3); values marked with the different letters are significantly different (P ≤ 0.05).

Mentions: The ability of fungal polysaccharides to reduce levels of cholesterol and the lipids in blood remains one of the important pharmacological properties. Chen and Huang [45] conducted experiments proving the ability of β-glucans to reduce cholesterol levels in blood by partial inhibition of absorption thereof. We confirmed that exopolysaccharides from G. applanatum were able to bind in vitro cholesterol and triglycerides (Figure 7(a)). The amount of bound cholesterol increased during the incubation time (39.2% after 2 hours and 57.9% after 24 hours of incubation). This correlation was not observed for triglycerides, where the level of bound substances was stable despite the time of incubation (2 hours, 43.3% and 24 hours, 41.6%). The conducted experiments also proved the possibility of glucose binding to the tested GpEPS. The incorporated glucose level was amounted to 26.6% and was independent of the incubation time. Magnesium and iron ions attachment ability (Figure 7(b)) revealed weak capacity of exopolysaccharides to absorption of these substances (17.4% for Mg2+ and 14.1% for Fe2+). In conclusion, all these data indicate that exopolysaccharides extracted from G. applanatum possess a high capability of binding cholesterol and triglycerides; however, further tests in vivo are required to confirm their hypocholesterolemic properties.


Exopolysaccharide from Ganoderma applanatum as a promising bioactive compound with cytostatic and antibacterial properties.

Osińska-Jaroszuk M, Jaszek M, Mizerska-Dudka M, Błachowicz A, Rejczak TP, Janusz G, Wydrych J, Polak J, Jarosz-Wilkołazka A, Kandefer-Szerszeń M - Biomed Res Int (2014)

Testing the ability of exopolysaccharides from G. applanatum (GpEPS)to binding: cholesterol, triglycerides, glucose, (a) and magnesium and iron ions (b) expressed as a percentage of the test substance bound to exopolysaccharides. All results are expressed as mean ± SD from three experiments (n = 3); values marked with the different letters are significantly different (P ≤ 0.05).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4120920&req=5

fig7: Testing the ability of exopolysaccharides from G. applanatum (GpEPS)to binding: cholesterol, triglycerides, glucose, (a) and magnesium and iron ions (b) expressed as a percentage of the test substance bound to exopolysaccharides. All results are expressed as mean ± SD from three experiments (n = 3); values marked with the different letters are significantly different (P ≤ 0.05).
Mentions: The ability of fungal polysaccharides to reduce levels of cholesterol and the lipids in blood remains one of the important pharmacological properties. Chen and Huang [45] conducted experiments proving the ability of β-glucans to reduce cholesterol levels in blood by partial inhibition of absorption thereof. We confirmed that exopolysaccharides from G. applanatum were able to bind in vitro cholesterol and triglycerides (Figure 7(a)). The amount of bound cholesterol increased during the incubation time (39.2% after 2 hours and 57.9% after 24 hours of incubation). This correlation was not observed for triglycerides, where the level of bound substances was stable despite the time of incubation (2 hours, 43.3% and 24 hours, 41.6%). The conducted experiments also proved the possibility of glucose binding to the tested GpEPS. The incorporated glucose level was amounted to 26.6% and was independent of the incubation time. Magnesium and iron ions attachment ability (Figure 7(b)) revealed weak capacity of exopolysaccharides to absorption of these substances (17.4% for Mg2+ and 14.1% for Fe2+). In conclusion, all these data indicate that exopolysaccharides extracted from G. applanatum possess a high capability of binding cholesterol and triglycerides; however, further tests in vivo are required to confirm their hypocholesterolemic properties.

Bottom Line: A new exopolysaccharide preparation isolated from stationary cultures of the white rot fungus Ganoderma applanatum (GpEPS) was tested in terms of its bioactive properties including its cytotoxic and immunostimulatory effect.The GpEPS showed antibacterial properties against Staphyloccoccus aureus and a toxic effect against Vibrio fischeri cells (82.8% cell damage).High cholesterol-binding capacity and triglycerides-binding capacity (57.9% and 41.6% after 24 h of incubation with the tested substances, resp.) were also detected for the investigated samples of GpEPS.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Maria Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland.

ABSTRACT
A new exopolysaccharide preparation isolated from stationary cultures of the white rot fungus Ganoderma applanatum (GpEPS) was tested in terms of its bioactive properties including its cytotoxic and immunostimulatory effect. The results indicate that the tested GpEPS (at concentrations above 22.85 µg/mL and 228.5 µg/mL) may exhibit selective activity against tumor cells (cell lines SiHa) and stimulate production of TNF-α THP-1-derived macrophages at the level of 752.17 pg/mL. The GpEPS showed antibacterial properties against Staphyloccoccus aureus and a toxic effect against Vibrio fischeri cells (82.8% cell damage). High cholesterol-binding capacity and triglycerides-binding capacity (57.9% and 41.6% after 24 h of incubation with the tested substances, resp.) were also detected for the investigated samples of GpEPS.

Show MeSH
Related in: MedlinePlus