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Exopolysaccharide from Ganoderma applanatum as a promising bioactive compound with cytostatic and antibacterial properties.

Osińska-Jaroszuk M, Jaszek M, Mizerska-Dudka M, Błachowicz A, Rejczak TP, Janusz G, Wydrych J, Polak J, Jarosz-Wilkołazka A, Kandefer-Szerszeń M - Biomed Res Int (2014)

Bottom Line: A new exopolysaccharide preparation isolated from stationary cultures of the white rot fungus Ganoderma applanatum (GpEPS) was tested in terms of its bioactive properties including its cytotoxic and immunostimulatory effect.The GpEPS showed antibacterial properties against Staphyloccoccus aureus and a toxic effect against Vibrio fischeri cells (82.8% cell damage).High cholesterol-binding capacity and triglycerides-binding capacity (57.9% and 41.6% after 24 h of incubation with the tested substances, resp.) were also detected for the investigated samples of GpEPS.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Maria Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland.

ABSTRACT
A new exopolysaccharide preparation isolated from stationary cultures of the white rot fungus Ganoderma applanatum (GpEPS) was tested in terms of its bioactive properties including its cytotoxic and immunostimulatory effect. The results indicate that the tested GpEPS (at concentrations above 22.85 µg/mL and 228.5 µg/mL) may exhibit selective activity against tumor cells (cell lines SiHa) and stimulate production of TNF-α THP-1-derived macrophages at the level of 752.17 pg/mL. The GpEPS showed antibacterial properties against Staphyloccoccus aureus and a toxic effect against Vibrio fischeri cells (82.8% cell damage). High cholesterol-binding capacity and triglycerides-binding capacity (57.9% and 41.6% after 24 h of incubation with the tested substances, resp.) were also detected for the investigated samples of GpEPS.

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Proliferative activity of carcinoma cell lines (SiHa and Ca Ski) and human skin fibroblast (HSF) in the presence of exopolysaccharides from G. applanatum (GpEPS).Each value is expressed as mean ± SD (n = 3).
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fig4: Proliferative activity of carcinoma cell lines (SiHa and Ca Ski) and human skin fibroblast (HSF) in the presence of exopolysaccharides from G. applanatum (GpEPS).Each value is expressed as mean ± SD (n = 3).

Mentions: In addition to the cytotoxic activity, antiproliferation activity of exopolysaccharides was also determined. In the presence of the exopolysaccharide from G. applanatum, there were no statistically significant changes in cell proliferation activity of the tested cell lines (HSF, SiHA, Ca Ski). The differences in proliferative activity in comparison with the control cells were approximately 4–10% for HSF, 4–13% for Ca Ski, and 2–13% for SiHa cell lines (Figure 4).


Exopolysaccharide from Ganoderma applanatum as a promising bioactive compound with cytostatic and antibacterial properties.

Osińska-Jaroszuk M, Jaszek M, Mizerska-Dudka M, Błachowicz A, Rejczak TP, Janusz G, Wydrych J, Polak J, Jarosz-Wilkołazka A, Kandefer-Szerszeń M - Biomed Res Int (2014)

Proliferative activity of carcinoma cell lines (SiHa and Ca Ski) and human skin fibroblast (HSF) in the presence of exopolysaccharides from G. applanatum (GpEPS).Each value is expressed as mean ± SD (n = 3).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4120920&req=5

fig4: Proliferative activity of carcinoma cell lines (SiHa and Ca Ski) and human skin fibroblast (HSF) in the presence of exopolysaccharides from G. applanatum (GpEPS).Each value is expressed as mean ± SD (n = 3).
Mentions: In addition to the cytotoxic activity, antiproliferation activity of exopolysaccharides was also determined. In the presence of the exopolysaccharide from G. applanatum, there were no statistically significant changes in cell proliferation activity of the tested cell lines (HSF, SiHA, Ca Ski). The differences in proliferative activity in comparison with the control cells were approximately 4–10% for HSF, 4–13% for Ca Ski, and 2–13% for SiHa cell lines (Figure 4).

Bottom Line: A new exopolysaccharide preparation isolated from stationary cultures of the white rot fungus Ganoderma applanatum (GpEPS) was tested in terms of its bioactive properties including its cytotoxic and immunostimulatory effect.The GpEPS showed antibacterial properties against Staphyloccoccus aureus and a toxic effect against Vibrio fischeri cells (82.8% cell damage).High cholesterol-binding capacity and triglycerides-binding capacity (57.9% and 41.6% after 24 h of incubation with the tested substances, resp.) were also detected for the investigated samples of GpEPS.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Maria Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland.

ABSTRACT
A new exopolysaccharide preparation isolated from stationary cultures of the white rot fungus Ganoderma applanatum (GpEPS) was tested in terms of its bioactive properties including its cytotoxic and immunostimulatory effect. The results indicate that the tested GpEPS (at concentrations above 22.85 µg/mL and 228.5 µg/mL) may exhibit selective activity against tumor cells (cell lines SiHa) and stimulate production of TNF-α THP-1-derived macrophages at the level of 752.17 pg/mL. The GpEPS showed antibacterial properties against Staphyloccoccus aureus and a toxic effect against Vibrio fischeri cells (82.8% cell damage). High cholesterol-binding capacity and triglycerides-binding capacity (57.9% and 41.6% after 24 h of incubation with the tested substances, resp.) were also detected for the investigated samples of GpEPS.

Show MeSH
Related in: MedlinePlus