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Exopolysaccharide from Ganoderma applanatum as a promising bioactive compound with cytostatic and antibacterial properties.

Osińska-Jaroszuk M, Jaszek M, Mizerska-Dudka M, Błachowicz A, Rejczak TP, Janusz G, Wydrych J, Polak J, Jarosz-Wilkołazka A, Kandefer-Szerszeń M - Biomed Res Int (2014)

Bottom Line: A new exopolysaccharide preparation isolated from stationary cultures of the white rot fungus Ganoderma applanatum (GpEPS) was tested in terms of its bioactive properties including its cytotoxic and immunostimulatory effect.The GpEPS showed antibacterial properties against Staphyloccoccus aureus and a toxic effect against Vibrio fischeri cells (82.8% cell damage).High cholesterol-binding capacity and triglycerides-binding capacity (57.9% and 41.6% after 24 h of incubation with the tested substances, resp.) were also detected for the investigated samples of GpEPS.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Maria Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland.

ABSTRACT
A new exopolysaccharide preparation isolated from stationary cultures of the white rot fungus Ganoderma applanatum (GpEPS) was tested in terms of its bioactive properties including its cytotoxic and immunostimulatory effect. The results indicate that the tested GpEPS (at concentrations above 22.85 µg/mL and 228.5 µg/mL) may exhibit selective activity against tumor cells (cell lines SiHa) and stimulate production of TNF-α THP-1-derived macrophages at the level of 752.17 pg/mL. The GpEPS showed antibacterial properties against Staphyloccoccus aureus and a toxic effect against Vibrio fischeri cells (82.8% cell damage). High cholesterol-binding capacity and triglycerides-binding capacity (57.9% and 41.6% after 24 h of incubation with the tested substances, resp.) were also detected for the investigated samples of GpEPS.

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The cytotoxic effect of exopolysaccharides from G. applanatum (GpEPS) against carcinoma cell lines (SiHa and Ca Ski) and human skin fibroblast (HSF) after 24 h (a) and 48 h (b) incubation. Each value is expressed as mean ± SD (n = 3).
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fig3: The cytotoxic effect of exopolysaccharides from G. applanatum (GpEPS) against carcinoma cell lines (SiHa and Ca Ski) and human skin fibroblast (HSF) after 24 h (a) and 48 h (b) incubation. Each value is expressed as mean ± SD (n = 3).

Mentions: The crude exopolysaccharides extracted from G. applanatum were subjected to in vitro cytotoxicity assays against carcinoma cell lines (SiHa and Ca Ski) and a human skin fibroblast (HSF) line. After 24 hours of incubation, slight changes in cell viability were observed (HSF, SiHa, Ca Ski), but they were not statistically significant, compared to the control. In contrast, the results obtained after 48 hour incubation were more varied. Our results showed that the isolated polysaccharides exhibited cytotoxic activity against the SiHa carcinoma cell line. A 42.8% and 34% decrease in cell viability at 22.88 μg/mL and 228.5 μg/mL concentrations of the exopolysaccharides studied, respectively, was noted (Figures 3(a) and 3(b)). On the other hand, a ca. 97% and 76% increase in the metabolic activity of Ca Ski cells for the exopolysaccharide concentrations of 22.85 and 228.5 μg/mL, respectively, was observed. In turn, a ca. 10–36% increase in the activity was observed in the case of fibroblasts (HSF). To our knowledge, there are currently no reports on the cytotoxic properties of exopolysaccharides from G. applanatum. Li et al. [39] showed that the polysaccharides from G. atrium inhibited tumor growth in S180-bearing mice via induction of apoptosis through mitochondrial pathways and immunoenhancement effects. Recently, polysaccharide-protein peptide conjugates with anticancer or immunomodulation properties were isolated from G. lucidium. For example, a GIPP fraction (polysaccharide-peptide conjugation) was indicated to inhibit proliferation of HUVECs by inducing cell apoptosis and decrease the expression of secreted VEGF in human lung cancer cells [40, 41]. The results of our study indicate that the tested polysaccharide fraction of G. applanatum may exhibit selective cytotoxic activity against SiHa cell lines at concentration above 22.85 μg/mL.


Exopolysaccharide from Ganoderma applanatum as a promising bioactive compound with cytostatic and antibacterial properties.

Osińska-Jaroszuk M, Jaszek M, Mizerska-Dudka M, Błachowicz A, Rejczak TP, Janusz G, Wydrych J, Polak J, Jarosz-Wilkołazka A, Kandefer-Szerszeń M - Biomed Res Int (2014)

The cytotoxic effect of exopolysaccharides from G. applanatum (GpEPS) against carcinoma cell lines (SiHa and Ca Ski) and human skin fibroblast (HSF) after 24 h (a) and 48 h (b) incubation. Each value is expressed as mean ± SD (n = 3).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4120920&req=5

fig3: The cytotoxic effect of exopolysaccharides from G. applanatum (GpEPS) against carcinoma cell lines (SiHa and Ca Ski) and human skin fibroblast (HSF) after 24 h (a) and 48 h (b) incubation. Each value is expressed as mean ± SD (n = 3).
Mentions: The crude exopolysaccharides extracted from G. applanatum were subjected to in vitro cytotoxicity assays against carcinoma cell lines (SiHa and Ca Ski) and a human skin fibroblast (HSF) line. After 24 hours of incubation, slight changes in cell viability were observed (HSF, SiHa, Ca Ski), but they were not statistically significant, compared to the control. In contrast, the results obtained after 48 hour incubation were more varied. Our results showed that the isolated polysaccharides exhibited cytotoxic activity against the SiHa carcinoma cell line. A 42.8% and 34% decrease in cell viability at 22.88 μg/mL and 228.5 μg/mL concentrations of the exopolysaccharides studied, respectively, was noted (Figures 3(a) and 3(b)). On the other hand, a ca. 97% and 76% increase in the metabolic activity of Ca Ski cells for the exopolysaccharide concentrations of 22.85 and 228.5 μg/mL, respectively, was observed. In turn, a ca. 10–36% increase in the activity was observed in the case of fibroblasts (HSF). To our knowledge, there are currently no reports on the cytotoxic properties of exopolysaccharides from G. applanatum. Li et al. [39] showed that the polysaccharides from G. atrium inhibited tumor growth in S180-bearing mice via induction of apoptosis through mitochondrial pathways and immunoenhancement effects. Recently, polysaccharide-protein peptide conjugates with anticancer or immunomodulation properties were isolated from G. lucidium. For example, a GIPP fraction (polysaccharide-peptide conjugation) was indicated to inhibit proliferation of HUVECs by inducing cell apoptosis and decrease the expression of secreted VEGF in human lung cancer cells [40, 41]. The results of our study indicate that the tested polysaccharide fraction of G. applanatum may exhibit selective cytotoxic activity against SiHa cell lines at concentration above 22.85 μg/mL.

Bottom Line: A new exopolysaccharide preparation isolated from stationary cultures of the white rot fungus Ganoderma applanatum (GpEPS) was tested in terms of its bioactive properties including its cytotoxic and immunostimulatory effect.The GpEPS showed antibacterial properties against Staphyloccoccus aureus and a toxic effect against Vibrio fischeri cells (82.8% cell damage).High cholesterol-binding capacity and triglycerides-binding capacity (57.9% and 41.6% after 24 h of incubation with the tested substances, resp.) were also detected for the investigated samples of GpEPS.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Maria Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland.

ABSTRACT
A new exopolysaccharide preparation isolated from stationary cultures of the white rot fungus Ganoderma applanatum (GpEPS) was tested in terms of its bioactive properties including its cytotoxic and immunostimulatory effect. The results indicate that the tested GpEPS (at concentrations above 22.85 µg/mL and 228.5 µg/mL) may exhibit selective activity against tumor cells (cell lines SiHa) and stimulate production of TNF-α THP-1-derived macrophages at the level of 752.17 pg/mL. The GpEPS showed antibacterial properties against Staphyloccoccus aureus and a toxic effect against Vibrio fischeri cells (82.8% cell damage). High cholesterol-binding capacity and triglycerides-binding capacity (57.9% and 41.6% after 24 h of incubation with the tested substances, resp.) were also detected for the investigated samples of GpEPS.

Show MeSH
Related in: MedlinePlus