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Exopolysaccharide from Ganoderma applanatum as a promising bioactive compound with cytostatic and antibacterial properties.

Osińska-Jaroszuk M, Jaszek M, Mizerska-Dudka M, Błachowicz A, Rejczak TP, Janusz G, Wydrych J, Polak J, Jarosz-Wilkołazka A, Kandefer-Szerszeń M - Biomed Res Int (2014)

Bottom Line: A new exopolysaccharide preparation isolated from stationary cultures of the white rot fungus Ganoderma applanatum (GpEPS) was tested in terms of its bioactive properties including its cytotoxic and immunostimulatory effect.The GpEPS showed antibacterial properties against Staphyloccoccus aureus and a toxic effect against Vibrio fischeri cells (82.8% cell damage).High cholesterol-binding capacity and triglycerides-binding capacity (57.9% and 41.6% after 24 h of incubation with the tested substances, resp.) were also detected for the investigated samples of GpEPS.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Maria Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland.

ABSTRACT
A new exopolysaccharide preparation isolated from stationary cultures of the white rot fungus Ganoderma applanatum (GpEPS) was tested in terms of its bioactive properties including its cytotoxic and immunostimulatory effect. The results indicate that the tested GpEPS (at concentrations above 22.85 µg/mL and 228.5 µg/mL) may exhibit selective activity against tumor cells (cell lines SiHa) and stimulate production of TNF-α THP-1-derived macrophages at the level of 752.17 pg/mL. The GpEPS showed antibacterial properties against Staphyloccoccus aureus and a toxic effect against Vibrio fischeri cells (82.8% cell damage). High cholesterol-binding capacity and triglycerides-binding capacity (57.9% and 41.6% after 24 h of incubation with the tested substances, resp.) were also detected for the investigated samples of GpEPS.

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Morphology of exopolysaccharides fibers using confocal laser scanning microscopy. The tested samples of GpEPS were stained with Fluorescence Brightener 28 commonly used in order to detection of β-linked polysaccharides. For visualization of the GpEPS, the inverted microscope Axiovert 200 M equipped with an LSM 5 Pascal head (with magnification 200x) was used. The letter (A) indicates the luminous fibers exhibiting visible β-linked polysaccharide fragments.
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fig2: Morphology of exopolysaccharides fibers using confocal laser scanning microscopy. The tested samples of GpEPS were stained with Fluorescence Brightener 28 commonly used in order to detection of β-linked polysaccharides. For visualization of the GpEPS, the inverted microscope Axiovert 200 M equipped with an LSM 5 Pascal head (with magnification 200x) was used. The letter (A) indicates the luminous fibers exhibiting visible β-linked polysaccharide fragments.

Mentions: The FT-IR spectrum of the ethanol-extracted exopolysaccharides of G. applanatum showed a typical carbohydrate pattern (Figure 1). The absorption band at 3292.3 cm−1 indicates the presence of the hydroxyl group (–OH) characteristic for molecular interactions of polysaccharide chains [32]. The two bands towards 1661.6 and 1437.6 cm−1 are correlated with the presence of the deprotonated carboxylic group (–COO−). The bands at 1026.9, 1132, and 1186 cm−1 suggested the presence of C–O bands, and the band at 1073 cm−1 was characteristic of the presence of β-glucans [33, 34]. Additionally, a characteristic peak at 840.8 cm−1 indicating α-linked glycosyl residues was observed by Kozarski et al. [13]. In addition, staining of the exopolysaccharides with Fluorescence Brightener 28 confirmed the presence of β-linked bonds in the polysaccharides studied (Figure 2). Chemical characterization of the exopolysaccharides properties is presented in Table 1.


Exopolysaccharide from Ganoderma applanatum as a promising bioactive compound with cytostatic and antibacterial properties.

Osińska-Jaroszuk M, Jaszek M, Mizerska-Dudka M, Błachowicz A, Rejczak TP, Janusz G, Wydrych J, Polak J, Jarosz-Wilkołazka A, Kandefer-Szerszeń M - Biomed Res Int (2014)

Morphology of exopolysaccharides fibers using confocal laser scanning microscopy. The tested samples of GpEPS were stained with Fluorescence Brightener 28 commonly used in order to detection of β-linked polysaccharides. For visualization of the GpEPS, the inverted microscope Axiovert 200 M equipped with an LSM 5 Pascal head (with magnification 200x) was used. The letter (A) indicates the luminous fibers exhibiting visible β-linked polysaccharide fragments.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4120920&req=5

fig2: Morphology of exopolysaccharides fibers using confocal laser scanning microscopy. The tested samples of GpEPS were stained with Fluorescence Brightener 28 commonly used in order to detection of β-linked polysaccharides. For visualization of the GpEPS, the inverted microscope Axiovert 200 M equipped with an LSM 5 Pascal head (with magnification 200x) was used. The letter (A) indicates the luminous fibers exhibiting visible β-linked polysaccharide fragments.
Mentions: The FT-IR spectrum of the ethanol-extracted exopolysaccharides of G. applanatum showed a typical carbohydrate pattern (Figure 1). The absorption band at 3292.3 cm−1 indicates the presence of the hydroxyl group (–OH) characteristic for molecular interactions of polysaccharide chains [32]. The two bands towards 1661.6 and 1437.6 cm−1 are correlated with the presence of the deprotonated carboxylic group (–COO−). The bands at 1026.9, 1132, and 1186 cm−1 suggested the presence of C–O bands, and the band at 1073 cm−1 was characteristic of the presence of β-glucans [33, 34]. Additionally, a characteristic peak at 840.8 cm−1 indicating α-linked glycosyl residues was observed by Kozarski et al. [13]. In addition, staining of the exopolysaccharides with Fluorescence Brightener 28 confirmed the presence of β-linked bonds in the polysaccharides studied (Figure 2). Chemical characterization of the exopolysaccharides properties is presented in Table 1.

Bottom Line: A new exopolysaccharide preparation isolated from stationary cultures of the white rot fungus Ganoderma applanatum (GpEPS) was tested in terms of its bioactive properties including its cytotoxic and immunostimulatory effect.The GpEPS showed antibacterial properties against Staphyloccoccus aureus and a toxic effect against Vibrio fischeri cells (82.8% cell damage).High cholesterol-binding capacity and triglycerides-binding capacity (57.9% and 41.6% after 24 h of incubation with the tested substances, resp.) were also detected for the investigated samples of GpEPS.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Maria Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland.

ABSTRACT
A new exopolysaccharide preparation isolated from stationary cultures of the white rot fungus Ganoderma applanatum (GpEPS) was tested in terms of its bioactive properties including its cytotoxic and immunostimulatory effect. The results indicate that the tested GpEPS (at concentrations above 22.85 µg/mL and 228.5 µg/mL) may exhibit selective activity against tumor cells (cell lines SiHa) and stimulate production of TNF-α THP-1-derived macrophages at the level of 752.17 pg/mL. The GpEPS showed antibacterial properties against Staphyloccoccus aureus and a toxic effect against Vibrio fischeri cells (82.8% cell damage). High cholesterol-binding capacity and triglycerides-binding capacity (57.9% and 41.6% after 24 h of incubation with the tested substances, resp.) were also detected for the investigated samples of GpEPS.

Show MeSH
Related in: MedlinePlus