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A Study on the Effect of Neurogenesis and Regulation of GSK3β/PP2A Expression in Acupuncture Treatment of Neural Functional Damage Caused by Focal Ischemia in MCAO Rats.

Luo D, Fan X, Ma C, Fan T, Wang X, Chang N, Li L, Zhang Y, Meng Z, Wang S, Shi X - Evid Based Complement Alternat Med (2014)

Bottom Line: 170 SD rats were randomly divided to five groups.Serious neural functional damage and sharp decrease of cerebral blood flow, obvious infarction volume, increased nestin mRNA expression, and immunopositive cells population (nestin(+), BrdU(+) and nestin/BrdU(+)) were found in MCAO rats which had not been observed in normal group and sham-operated group.In conclusion, the present study indicates that the underlying mechanism of acupuncture treatment on neural functional damage caused by focal ischemia injury is a multiple interaction which may involve improved cerebral blood supply, neurogenesis, and regulation of gene transcription or GSK-3β and PP2A expression in MCAO rats.

View Article: PubMed Central - PubMed

Affiliation: Postgraduate Department of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.

ABSTRACT
170 SD rats were randomly divided to five groups. Rats in model group, no-acupuncture group, and acupuncture group were subjected to MCAO surgery. Acupuncture group received 3 consecutive acupuncture treatments at a parameter that deep in 2 mm towards apex nasi and thrust/lifted at 3 times per second for 1 minute, while model group and no-acupuncture group were no-intervention control groups. Serious neural functional damage and sharp decrease of cerebral blood flow, obvious infarction volume, increased nestin mRNA expression, and immunopositive cells population (nestin(+), BrdU(+) and nestin/BrdU(+)) were found in MCAO rats which had not been observed in normal group and sham-operated group. However, the damage was attenuated by rat's "self-healing" capacity 3 days after MCAO. And the "self-healing" capacity can be strengthen by acupuncture treatment through increasing cerebral blood flow, neurogenesis, and regulation of gene transcription or GSK-3β and PP2A expression. In conclusion, the present study indicates that the underlying mechanism of acupuncture treatment on neural functional damage caused by focal ischemia injury is a multiple interaction which may involve improved cerebral blood supply, neurogenesis, and regulation of gene transcription or GSK-3β and PP2A expression in MCAO rats.

No MeSH data available.


Related in: MedlinePlus

Acupuncture made a positive regulation of GSK-3β, PP2A, and G/P. GSK-3β expression and G/P significantly increased in MCAO groups but decreased in acupuncture group (P < 0.05). PP2A expression showed a reverse tendency. The expression of GSK-3β and PP2A had a temporal profile. But acupuncture had a positive regulation for GSK-3β, PP2A expression, and G/P (P < 0.05).
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fig7: Acupuncture made a positive regulation of GSK-3β, PP2A, and G/P. GSK-3β expression and G/P significantly increased in MCAO groups but decreased in acupuncture group (P < 0.05). PP2A expression showed a reverse tendency. The expression of GSK-3β and PP2A had a temporal profile. But acupuncture had a positive regulation for GSK-3β, PP2A expression, and G/P (P < 0.05).

Mentions: Microarray analysis was shown biological processes of metabolism, phosphorylation, and cell proliferation relevant to neural protective effect after ischemia. Thus, to verify the microarray analysis results, we evaluated GSK-3β and PP2A expression (which well-accepted have closely connection of biological processes mentioned above) in different cerebral regions of rats by ELISA. As demonstrated in Figure 7(a), GSK-3β expression robust increased in MCAO groups, whereas PP2A expression conspicuous decreased. And expression of GSK-3β in no-acupuncture was decreased meanwhile increased of PP2A expression compared to model group (P < 0.05). However, acupuncture shown a regulation effects on expression tendency of GSK-3β and PP2A: it enhanced the increasing of GSK-3β while attenuated decreasing of PP2A (P < 0.05). And the similar tendency of GSK-3β and PP2A expression was found in cortex, hippocampus and striatum. The ratio of GSK-3β and PP2A was also dramatically elevated post-ischemia, but decreased in no-acupuncture group and acupuncture group (P < 0.05). The results revealed that it was a temporal profile of GSK-3β and PP2A expression, and the ratio of GSK-3β and PP2A (G/P) positive regulated by acupuncture stimulation.


A Study on the Effect of Neurogenesis and Regulation of GSK3β/PP2A Expression in Acupuncture Treatment of Neural Functional Damage Caused by Focal Ischemia in MCAO Rats.

Luo D, Fan X, Ma C, Fan T, Wang X, Chang N, Li L, Zhang Y, Meng Z, Wang S, Shi X - Evid Based Complement Alternat Med (2014)

Acupuncture made a positive regulation of GSK-3β, PP2A, and G/P. GSK-3β expression and G/P significantly increased in MCAO groups but decreased in acupuncture group (P < 0.05). PP2A expression showed a reverse tendency. The expression of GSK-3β and PP2A had a temporal profile. But acupuncture had a positive regulation for GSK-3β, PP2A expression, and G/P (P < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4120913&req=5

fig7: Acupuncture made a positive regulation of GSK-3β, PP2A, and G/P. GSK-3β expression and G/P significantly increased in MCAO groups but decreased in acupuncture group (P < 0.05). PP2A expression showed a reverse tendency. The expression of GSK-3β and PP2A had a temporal profile. But acupuncture had a positive regulation for GSK-3β, PP2A expression, and G/P (P < 0.05).
Mentions: Microarray analysis was shown biological processes of metabolism, phosphorylation, and cell proliferation relevant to neural protective effect after ischemia. Thus, to verify the microarray analysis results, we evaluated GSK-3β and PP2A expression (which well-accepted have closely connection of biological processes mentioned above) in different cerebral regions of rats by ELISA. As demonstrated in Figure 7(a), GSK-3β expression robust increased in MCAO groups, whereas PP2A expression conspicuous decreased. And expression of GSK-3β in no-acupuncture was decreased meanwhile increased of PP2A expression compared to model group (P < 0.05). However, acupuncture shown a regulation effects on expression tendency of GSK-3β and PP2A: it enhanced the increasing of GSK-3β while attenuated decreasing of PP2A (P < 0.05). And the similar tendency of GSK-3β and PP2A expression was found in cortex, hippocampus and striatum. The ratio of GSK-3β and PP2A was also dramatically elevated post-ischemia, but decreased in no-acupuncture group and acupuncture group (P < 0.05). The results revealed that it was a temporal profile of GSK-3β and PP2A expression, and the ratio of GSK-3β and PP2A (G/P) positive regulated by acupuncture stimulation.

Bottom Line: 170 SD rats were randomly divided to five groups.Serious neural functional damage and sharp decrease of cerebral blood flow, obvious infarction volume, increased nestin mRNA expression, and immunopositive cells population (nestin(+), BrdU(+) and nestin/BrdU(+)) were found in MCAO rats which had not been observed in normal group and sham-operated group.In conclusion, the present study indicates that the underlying mechanism of acupuncture treatment on neural functional damage caused by focal ischemia injury is a multiple interaction which may involve improved cerebral blood supply, neurogenesis, and regulation of gene transcription or GSK-3β and PP2A expression in MCAO rats.

View Article: PubMed Central - PubMed

Affiliation: Postgraduate Department of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.

ABSTRACT
170 SD rats were randomly divided to five groups. Rats in model group, no-acupuncture group, and acupuncture group were subjected to MCAO surgery. Acupuncture group received 3 consecutive acupuncture treatments at a parameter that deep in 2 mm towards apex nasi and thrust/lifted at 3 times per second for 1 minute, while model group and no-acupuncture group were no-intervention control groups. Serious neural functional damage and sharp decrease of cerebral blood flow, obvious infarction volume, increased nestin mRNA expression, and immunopositive cells population (nestin(+), BrdU(+) and nestin/BrdU(+)) were found in MCAO rats which had not been observed in normal group and sham-operated group. However, the damage was attenuated by rat's "self-healing" capacity 3 days after MCAO. And the "self-healing" capacity can be strengthen by acupuncture treatment through increasing cerebral blood flow, neurogenesis, and regulation of gene transcription or GSK-3β and PP2A expression. In conclusion, the present study indicates that the underlying mechanism of acupuncture treatment on neural functional damage caused by focal ischemia injury is a multiple interaction which may involve improved cerebral blood supply, neurogenesis, and regulation of gene transcription or GSK-3β and PP2A expression in MCAO rats.

No MeSH data available.


Related in: MedlinePlus