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A Study on the Effect of Neurogenesis and Regulation of GSK3β/PP2A Expression in Acupuncture Treatment of Neural Functional Damage Caused by Focal Ischemia in MCAO Rats.

Luo D, Fan X, Ma C, Fan T, Wang X, Chang N, Li L, Zhang Y, Meng Z, Wang S, Shi X - Evid Based Complement Alternat Med (2014)

Bottom Line: 170 SD rats were randomly divided to five groups.Serious neural functional damage and sharp decrease of cerebral blood flow, obvious infarction volume, increased nestin mRNA expression, and immunopositive cells population (nestin(+), BrdU(+) and nestin/BrdU(+)) were found in MCAO rats which had not been observed in normal group and sham-operated group.In conclusion, the present study indicates that the underlying mechanism of acupuncture treatment on neural functional damage caused by focal ischemia injury is a multiple interaction which may involve improved cerebral blood supply, neurogenesis, and regulation of gene transcription or GSK-3β and PP2A expression in MCAO rats.

View Article: PubMed Central - PubMed

Affiliation: Postgraduate Department of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.

ABSTRACT
170 SD rats were randomly divided to five groups. Rats in model group, no-acupuncture group, and acupuncture group were subjected to MCAO surgery. Acupuncture group received 3 consecutive acupuncture treatments at a parameter that deep in 2 mm towards apex nasi and thrust/lifted at 3 times per second for 1 minute, while model group and no-acupuncture group were no-intervention control groups. Serious neural functional damage and sharp decrease of cerebral blood flow, obvious infarction volume, increased nestin mRNA expression, and immunopositive cells population (nestin(+), BrdU(+) and nestin/BrdU(+)) were found in MCAO rats which had not been observed in normal group and sham-operated group. However, the damage was attenuated by rat's "self-healing" capacity 3 days after MCAO. And the "self-healing" capacity can be strengthen by acupuncture treatment through increasing cerebral blood flow, neurogenesis, and regulation of gene transcription or GSK-3β and PP2A expression. In conclusion, the present study indicates that the underlying mechanism of acupuncture treatment on neural functional damage caused by focal ischemia injury is a multiple interaction which may involve improved cerebral blood supply, neurogenesis, and regulation of gene transcription or GSK-3β and PP2A expression in MCAO rats.

No MeSH data available.


Related in: MedlinePlus

Effects of acupuncture on increasing population of immunopositive cells in penumbra: 5 visual regions in penumbra of each slice were selected for cell counting under a 400x microscope (n = 6). (a) Representative images of Immunofluorescence double-staining from model group, no-acupuncture group, and acupuncture group: a1, b1, and c1 labeled for BrdU (green). a2, b2, and c2 labeled for nestin (red). a3, b3, and c3 colabeled for BrdU/nestin. White arrows points to the BrdU/nestin+ cells. (b), (c), and (d) Quantization of BrdU+, nestin+, and BrdU/nestin+ cells: immunopositive cells were found in MCAO groups while not been found in normal and sham-operated group. Acupuncture enhanced BrdU+, nestin+, and BrdU/nestin+ labeled cells proliferation (▲P < 0.05, acupuncture group versus model group and no-acupuncture group). More BrdU+ labeled cells in no-acupuncture group than model group (★P < 0.05).
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fig5: Effects of acupuncture on increasing population of immunopositive cells in penumbra: 5 visual regions in penumbra of each slice were selected for cell counting under a 400x microscope (n = 6). (a) Representative images of Immunofluorescence double-staining from model group, no-acupuncture group, and acupuncture group: a1, b1, and c1 labeled for BrdU (green). a2, b2, and c2 labeled for nestin (red). a3, b3, and c3 colabeled for BrdU/nestin. White arrows points to the BrdU/nestin+ cells. (b), (c), and (d) Quantization of BrdU+, nestin+, and BrdU/nestin+ cells: immunopositive cells were found in MCAO groups while not been found in normal and sham-operated group. Acupuncture enhanced BrdU+, nestin+, and BrdU/nestin+ labeled cells proliferation (▲P < 0.05, acupuncture group versus model group and no-acupuncture group). More BrdU+ labeled cells in no-acupuncture group than model group (★P < 0.05).

Mentions: By immunofluorescence double-staining, a large number of BrdU+, nestin+, and BrdU/nestin+ cells in MCAO groups were observed as shown in Figure 5, whereas no immune-positive cells were found in normal and sham-operated group. There was also a significant increase of immunopositive cells in acupuncture group versus model group and no-acupuncture group (shown in Figures 5(b), 5(c), and 5(d)), suggesting acupuncture treatment induced proliferation of neural stem cells in a certain extent. And BrdU+ cells obviously increased in no-acupuncture group compared to model group. However, no distinct difference was found in nestin+ and BrdU/nestin+ between no-acupuncture group and model group (shown in Figures 5(c) and 5(d)).


A Study on the Effect of Neurogenesis and Regulation of GSK3β/PP2A Expression in Acupuncture Treatment of Neural Functional Damage Caused by Focal Ischemia in MCAO Rats.

Luo D, Fan X, Ma C, Fan T, Wang X, Chang N, Li L, Zhang Y, Meng Z, Wang S, Shi X - Evid Based Complement Alternat Med (2014)

Effects of acupuncture on increasing population of immunopositive cells in penumbra: 5 visual regions in penumbra of each slice were selected for cell counting under a 400x microscope (n = 6). (a) Representative images of Immunofluorescence double-staining from model group, no-acupuncture group, and acupuncture group: a1, b1, and c1 labeled for BrdU (green). a2, b2, and c2 labeled for nestin (red). a3, b3, and c3 colabeled for BrdU/nestin. White arrows points to the BrdU/nestin+ cells. (b), (c), and (d) Quantization of BrdU+, nestin+, and BrdU/nestin+ cells: immunopositive cells were found in MCAO groups while not been found in normal and sham-operated group. Acupuncture enhanced BrdU+, nestin+, and BrdU/nestin+ labeled cells proliferation (▲P < 0.05, acupuncture group versus model group and no-acupuncture group). More BrdU+ labeled cells in no-acupuncture group than model group (★P < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4120913&req=5

fig5: Effects of acupuncture on increasing population of immunopositive cells in penumbra: 5 visual regions in penumbra of each slice were selected for cell counting under a 400x microscope (n = 6). (a) Representative images of Immunofluorescence double-staining from model group, no-acupuncture group, and acupuncture group: a1, b1, and c1 labeled for BrdU (green). a2, b2, and c2 labeled for nestin (red). a3, b3, and c3 colabeled for BrdU/nestin. White arrows points to the BrdU/nestin+ cells. (b), (c), and (d) Quantization of BrdU+, nestin+, and BrdU/nestin+ cells: immunopositive cells were found in MCAO groups while not been found in normal and sham-operated group. Acupuncture enhanced BrdU+, nestin+, and BrdU/nestin+ labeled cells proliferation (▲P < 0.05, acupuncture group versus model group and no-acupuncture group). More BrdU+ labeled cells in no-acupuncture group than model group (★P < 0.05).
Mentions: By immunofluorescence double-staining, a large number of BrdU+, nestin+, and BrdU/nestin+ cells in MCAO groups were observed as shown in Figure 5, whereas no immune-positive cells were found in normal and sham-operated group. There was also a significant increase of immunopositive cells in acupuncture group versus model group and no-acupuncture group (shown in Figures 5(b), 5(c), and 5(d)), suggesting acupuncture treatment induced proliferation of neural stem cells in a certain extent. And BrdU+ cells obviously increased in no-acupuncture group compared to model group. However, no distinct difference was found in nestin+ and BrdU/nestin+ between no-acupuncture group and model group (shown in Figures 5(c) and 5(d)).

Bottom Line: 170 SD rats were randomly divided to five groups.Serious neural functional damage and sharp decrease of cerebral blood flow, obvious infarction volume, increased nestin mRNA expression, and immunopositive cells population (nestin(+), BrdU(+) and nestin/BrdU(+)) were found in MCAO rats which had not been observed in normal group and sham-operated group.In conclusion, the present study indicates that the underlying mechanism of acupuncture treatment on neural functional damage caused by focal ischemia injury is a multiple interaction which may involve improved cerebral blood supply, neurogenesis, and regulation of gene transcription or GSK-3β and PP2A expression in MCAO rats.

View Article: PubMed Central - PubMed

Affiliation: Postgraduate Department of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.

ABSTRACT
170 SD rats were randomly divided to five groups. Rats in model group, no-acupuncture group, and acupuncture group were subjected to MCAO surgery. Acupuncture group received 3 consecutive acupuncture treatments at a parameter that deep in 2 mm towards apex nasi and thrust/lifted at 3 times per second for 1 minute, while model group and no-acupuncture group were no-intervention control groups. Serious neural functional damage and sharp decrease of cerebral blood flow, obvious infarction volume, increased nestin mRNA expression, and immunopositive cells population (nestin(+), BrdU(+) and nestin/BrdU(+)) were found in MCAO rats which had not been observed in normal group and sham-operated group. However, the damage was attenuated by rat's "self-healing" capacity 3 days after MCAO. And the "self-healing" capacity can be strengthen by acupuncture treatment through increasing cerebral blood flow, neurogenesis, and regulation of gene transcription or GSK-3β and PP2A expression. In conclusion, the present study indicates that the underlying mechanism of acupuncture treatment on neural functional damage caused by focal ischemia injury is a multiple interaction which may involve improved cerebral blood supply, neurogenesis, and regulation of gene transcription or GSK-3β and PP2A expression in MCAO rats.

No MeSH data available.


Related in: MedlinePlus