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A Study on the Effect of Neurogenesis and Regulation of GSK3β/PP2A Expression in Acupuncture Treatment of Neural Functional Damage Caused by Focal Ischemia in MCAO Rats.

Luo D, Fan X, Ma C, Fan T, Wang X, Chang N, Li L, Zhang Y, Meng Z, Wang S, Shi X - Evid Based Complement Alternat Med (2014)

Bottom Line: 170 SD rats were randomly divided to five groups.Serious neural functional damage and sharp decrease of cerebral blood flow, obvious infarction volume, increased nestin mRNA expression, and immunopositive cells population (nestin(+), BrdU(+) and nestin/BrdU(+)) were found in MCAO rats which had not been observed in normal group and sham-operated group.In conclusion, the present study indicates that the underlying mechanism of acupuncture treatment on neural functional damage caused by focal ischemia injury is a multiple interaction which may involve improved cerebral blood supply, neurogenesis, and regulation of gene transcription or GSK-3β and PP2A expression in MCAO rats.

View Article: PubMed Central - PubMed

Affiliation: Postgraduate Department of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.

ABSTRACT
170 SD rats were randomly divided to five groups. Rats in model group, no-acupuncture group, and acupuncture group were subjected to MCAO surgery. Acupuncture group received 3 consecutive acupuncture treatments at a parameter that deep in 2 mm towards apex nasi and thrust/lifted at 3 times per second for 1 minute, while model group and no-acupuncture group were no-intervention control groups. Serious neural functional damage and sharp decrease of cerebral blood flow, obvious infarction volume, increased nestin mRNA expression, and immunopositive cells population (nestin(+), BrdU(+) and nestin/BrdU(+)) were found in MCAO rats which had not been observed in normal group and sham-operated group. However, the damage was attenuated by rat's "self-healing" capacity 3 days after MCAO. And the "self-healing" capacity can be strengthen by acupuncture treatment through increasing cerebral blood flow, neurogenesis, and regulation of gene transcription or GSK-3β and PP2A expression. In conclusion, the present study indicates that the underlying mechanism of acupuncture treatment on neural functional damage caused by focal ischemia injury is a multiple interaction which may involve improved cerebral blood supply, neurogenesis, and regulation of gene transcription or GSK-3β and PP2A expression in MCAO rats.

No MeSH data available.


Related in: MedlinePlus

Acupuncture attenuated neural functional deficit. Neural deficit score (NDS) was employed for evaluating the neural functional deficit (n = 18). The lower the score is, the worse the damage is. There were significant differences among MCAO groups: no-acupuncture group got higher scores than model group (★P < 0.05) suggesting a “self-repairing” ability of MCAO rats. Acupuncture group got the highest scores in MCAO group while lower scores in model and no-acupuncture group (▲P < 0.05) which meant that acupuncture attenuated neural damage after MCAO but did not reach to a normal level.
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fig2: Acupuncture attenuated neural functional deficit. Neural deficit score (NDS) was employed for evaluating the neural functional deficit (n = 18). The lower the score is, the worse the damage is. There were significant differences among MCAO groups: no-acupuncture group got higher scores than model group (★P < 0.05) suggesting a “self-repairing” ability of MCAO rats. Acupuncture group got the highest scores in MCAO group while lower scores in model and no-acupuncture group (▲P < 0.05) which meant that acupuncture attenuated neural damage after MCAO but did not reach to a normal level.

Mentions: Neural functional deficit was evaluated by Zausinger et al. [17] 6 point scale at baseline and before euthanatized in MCAO groups and the corresponding time point of normal and sham-operated group. Mann-Whitney U test was conducted to analyze the neural deficit score. As shown in Figure 2, there were significant differences among MCAO groups and normal, sham-operate group (P < 0.05) which means MCAO caused a dramatic damage on neural function. Compared two no-intervention groups, no-acupuncture group shown an attenuation of neural damage to model group suggesting a self-repairing capacity of rat post ischemia infarction. But acupuncture group shown a distinct increased in NDS compared to other MCAO groups while still lower than normal and sham-operated group indicating that acupuncture relieves the neural functional damage but did not reach to a normal level.


A Study on the Effect of Neurogenesis and Regulation of GSK3β/PP2A Expression in Acupuncture Treatment of Neural Functional Damage Caused by Focal Ischemia in MCAO Rats.

Luo D, Fan X, Ma C, Fan T, Wang X, Chang N, Li L, Zhang Y, Meng Z, Wang S, Shi X - Evid Based Complement Alternat Med (2014)

Acupuncture attenuated neural functional deficit. Neural deficit score (NDS) was employed for evaluating the neural functional deficit (n = 18). The lower the score is, the worse the damage is. There were significant differences among MCAO groups: no-acupuncture group got higher scores than model group (★P < 0.05) suggesting a “self-repairing” ability of MCAO rats. Acupuncture group got the highest scores in MCAO group while lower scores in model and no-acupuncture group (▲P < 0.05) which meant that acupuncture attenuated neural damage after MCAO but did not reach to a normal level.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4120913&req=5

fig2: Acupuncture attenuated neural functional deficit. Neural deficit score (NDS) was employed for evaluating the neural functional deficit (n = 18). The lower the score is, the worse the damage is. There were significant differences among MCAO groups: no-acupuncture group got higher scores than model group (★P < 0.05) suggesting a “self-repairing” ability of MCAO rats. Acupuncture group got the highest scores in MCAO group while lower scores in model and no-acupuncture group (▲P < 0.05) which meant that acupuncture attenuated neural damage after MCAO but did not reach to a normal level.
Mentions: Neural functional deficit was evaluated by Zausinger et al. [17] 6 point scale at baseline and before euthanatized in MCAO groups and the corresponding time point of normal and sham-operated group. Mann-Whitney U test was conducted to analyze the neural deficit score. As shown in Figure 2, there were significant differences among MCAO groups and normal, sham-operate group (P < 0.05) which means MCAO caused a dramatic damage on neural function. Compared two no-intervention groups, no-acupuncture group shown an attenuation of neural damage to model group suggesting a self-repairing capacity of rat post ischemia infarction. But acupuncture group shown a distinct increased in NDS compared to other MCAO groups while still lower than normal and sham-operated group indicating that acupuncture relieves the neural functional damage but did not reach to a normal level.

Bottom Line: 170 SD rats were randomly divided to five groups.Serious neural functional damage and sharp decrease of cerebral blood flow, obvious infarction volume, increased nestin mRNA expression, and immunopositive cells population (nestin(+), BrdU(+) and nestin/BrdU(+)) were found in MCAO rats which had not been observed in normal group and sham-operated group.In conclusion, the present study indicates that the underlying mechanism of acupuncture treatment on neural functional damage caused by focal ischemia injury is a multiple interaction which may involve improved cerebral blood supply, neurogenesis, and regulation of gene transcription or GSK-3β and PP2A expression in MCAO rats.

View Article: PubMed Central - PubMed

Affiliation: Postgraduate Department of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.

ABSTRACT
170 SD rats were randomly divided to five groups. Rats in model group, no-acupuncture group, and acupuncture group were subjected to MCAO surgery. Acupuncture group received 3 consecutive acupuncture treatments at a parameter that deep in 2 mm towards apex nasi and thrust/lifted at 3 times per second for 1 minute, while model group and no-acupuncture group were no-intervention control groups. Serious neural functional damage and sharp decrease of cerebral blood flow, obvious infarction volume, increased nestin mRNA expression, and immunopositive cells population (nestin(+), BrdU(+) and nestin/BrdU(+)) were found in MCAO rats which had not been observed in normal group and sham-operated group. However, the damage was attenuated by rat's "self-healing" capacity 3 days after MCAO. And the "self-healing" capacity can be strengthen by acupuncture treatment through increasing cerebral blood flow, neurogenesis, and regulation of gene transcription or GSK-3β and PP2A expression. In conclusion, the present study indicates that the underlying mechanism of acupuncture treatment on neural functional damage caused by focal ischemia injury is a multiple interaction which may involve improved cerebral blood supply, neurogenesis, and regulation of gene transcription or GSK-3β and PP2A expression in MCAO rats.

No MeSH data available.


Related in: MedlinePlus