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A Study on the Effect of Neurogenesis and Regulation of GSK3β/PP2A Expression in Acupuncture Treatment of Neural Functional Damage Caused by Focal Ischemia in MCAO Rats.

Luo D, Fan X, Ma C, Fan T, Wang X, Chang N, Li L, Zhang Y, Meng Z, Wang S, Shi X - Evid Based Complement Alternat Med (2014)

Bottom Line: 170 SD rats were randomly divided to five groups.Serious neural functional damage and sharp decrease of cerebral blood flow, obvious infarction volume, increased nestin mRNA expression, and immunopositive cells population (nestin(+), BrdU(+) and nestin/BrdU(+)) were found in MCAO rats which had not been observed in normal group and sham-operated group.In conclusion, the present study indicates that the underlying mechanism of acupuncture treatment on neural functional damage caused by focal ischemia injury is a multiple interaction which may involve improved cerebral blood supply, neurogenesis, and regulation of gene transcription or GSK-3β and PP2A expression in MCAO rats.

View Article: PubMed Central - PubMed

Affiliation: Postgraduate Department of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.

ABSTRACT
170 SD rats were randomly divided to five groups. Rats in model group, no-acupuncture group, and acupuncture group were subjected to MCAO surgery. Acupuncture group received 3 consecutive acupuncture treatments at a parameter that deep in 2 mm towards apex nasi and thrust/lifted at 3 times per second for 1 minute, while model group and no-acupuncture group were no-intervention control groups. Serious neural functional damage and sharp decrease of cerebral blood flow, obvious infarction volume, increased nestin mRNA expression, and immunopositive cells population (nestin(+), BrdU(+) and nestin/BrdU(+)) were found in MCAO rats which had not been observed in normal group and sham-operated group. However, the damage was attenuated by rat's "self-healing" capacity 3 days after MCAO. And the "self-healing" capacity can be strengthen by acupuncture treatment through increasing cerebral blood flow, neurogenesis, and regulation of gene transcription or GSK-3β and PP2A expression. In conclusion, the present study indicates that the underlying mechanism of acupuncture treatment on neural functional damage caused by focal ischemia injury is a multiple interaction which may involve improved cerebral blood supply, neurogenesis, and regulation of gene transcription or GSK-3β and PP2A expression in MCAO rats.

No MeSH data available.


Related in: MedlinePlus

Schematic diagram for methodologies: a total of 170 adult male SD rats were randomly divided into normal, sham-operated, model, no-acupuncture, and acupuncture group. Rats in model group euthanatized immediately after MCAO while other groups at 3 days for subsequent investigation of neural deficit score (NDS), cerebral blood flow (CBF), nestin mRNA expression, microarray analysis, and enzyme-linked immunoabsorbent assay. Another sets of rats sacrificed 7 days for immunopositive cell counting.
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fig1: Schematic diagram for methodologies: a total of 170 adult male SD rats were randomly divided into normal, sham-operated, model, no-acupuncture, and acupuncture group. Rats in model group euthanatized immediately after MCAO while other groups at 3 days for subsequent investigation of neural deficit score (NDS), cerebral blood flow (CBF), nestin mRNA expression, microarray analysis, and enzyme-linked immunoabsorbent assay. Another sets of rats sacrificed 7 days for immunopositive cell counting.

Mentions: A total of 170 adult male Sprague-Dawley rats which were purchased from Experimental Animal Center of Academy of Military Medical Sciences weighting from 250 to 280 g were employed. All animals were housed in a conditioned environment (12 hour-light/12 hour-dark circle, humidity 55 ± 5%, and free access to food and water). All the experimental protocols were approved by local animal ethical committee and consistent with experimental animal use guidelines. Rats were randomly divided into five groups as normal group, sham-operated group, model group, no-acupuncture group, and acupuncture group. Rats in model group, no-acupuncture group, and acupuncture group subjected to MCAO surgery. Acupuncture group received 3 consecutive acupuncture treatments. The model group and no-acupuncture group were no-intervention control groups for acupuncture group. Rats in model group decapitated immediately after behavior measurement by neural deficit scores when recovering consciousness from MCAO surgery. But rats in no-acupuncture group were killed at the same time point as acupuncture group and the difference between two groups was received acupuncture treatment or not (one set sacrificed at 3 days after MCAO for CBF, IV, PCR, and gene microarray measurement while the other set killed at 7 days for immunopositive population counting). It was meant to investigate the immediate damage caused by ischemia and the “self-repairing” ability of MCAO rats. The experimental protocols were shown in Figure 1.


A Study on the Effect of Neurogenesis and Regulation of GSK3β/PP2A Expression in Acupuncture Treatment of Neural Functional Damage Caused by Focal Ischemia in MCAO Rats.

Luo D, Fan X, Ma C, Fan T, Wang X, Chang N, Li L, Zhang Y, Meng Z, Wang S, Shi X - Evid Based Complement Alternat Med (2014)

Schematic diagram for methodologies: a total of 170 adult male SD rats were randomly divided into normal, sham-operated, model, no-acupuncture, and acupuncture group. Rats in model group euthanatized immediately after MCAO while other groups at 3 days for subsequent investigation of neural deficit score (NDS), cerebral blood flow (CBF), nestin mRNA expression, microarray analysis, and enzyme-linked immunoabsorbent assay. Another sets of rats sacrificed 7 days for immunopositive cell counting.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4120913&req=5

fig1: Schematic diagram for methodologies: a total of 170 adult male SD rats were randomly divided into normal, sham-operated, model, no-acupuncture, and acupuncture group. Rats in model group euthanatized immediately after MCAO while other groups at 3 days for subsequent investigation of neural deficit score (NDS), cerebral blood flow (CBF), nestin mRNA expression, microarray analysis, and enzyme-linked immunoabsorbent assay. Another sets of rats sacrificed 7 days for immunopositive cell counting.
Mentions: A total of 170 adult male Sprague-Dawley rats which were purchased from Experimental Animal Center of Academy of Military Medical Sciences weighting from 250 to 280 g were employed. All animals were housed in a conditioned environment (12 hour-light/12 hour-dark circle, humidity 55 ± 5%, and free access to food and water). All the experimental protocols were approved by local animal ethical committee and consistent with experimental animal use guidelines. Rats were randomly divided into five groups as normal group, sham-operated group, model group, no-acupuncture group, and acupuncture group. Rats in model group, no-acupuncture group, and acupuncture group subjected to MCAO surgery. Acupuncture group received 3 consecutive acupuncture treatments. The model group and no-acupuncture group were no-intervention control groups for acupuncture group. Rats in model group decapitated immediately after behavior measurement by neural deficit scores when recovering consciousness from MCAO surgery. But rats in no-acupuncture group were killed at the same time point as acupuncture group and the difference between two groups was received acupuncture treatment or not (one set sacrificed at 3 days after MCAO for CBF, IV, PCR, and gene microarray measurement while the other set killed at 7 days for immunopositive population counting). It was meant to investigate the immediate damage caused by ischemia and the “self-repairing” ability of MCAO rats. The experimental protocols were shown in Figure 1.

Bottom Line: 170 SD rats were randomly divided to five groups.Serious neural functional damage and sharp decrease of cerebral blood flow, obvious infarction volume, increased nestin mRNA expression, and immunopositive cells population (nestin(+), BrdU(+) and nestin/BrdU(+)) were found in MCAO rats which had not been observed in normal group and sham-operated group.In conclusion, the present study indicates that the underlying mechanism of acupuncture treatment on neural functional damage caused by focal ischemia injury is a multiple interaction which may involve improved cerebral blood supply, neurogenesis, and regulation of gene transcription or GSK-3β and PP2A expression in MCAO rats.

View Article: PubMed Central - PubMed

Affiliation: Postgraduate Department of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.

ABSTRACT
170 SD rats were randomly divided to five groups. Rats in model group, no-acupuncture group, and acupuncture group were subjected to MCAO surgery. Acupuncture group received 3 consecutive acupuncture treatments at a parameter that deep in 2 mm towards apex nasi and thrust/lifted at 3 times per second for 1 minute, while model group and no-acupuncture group were no-intervention control groups. Serious neural functional damage and sharp decrease of cerebral blood flow, obvious infarction volume, increased nestin mRNA expression, and immunopositive cells population (nestin(+), BrdU(+) and nestin/BrdU(+)) were found in MCAO rats which had not been observed in normal group and sham-operated group. However, the damage was attenuated by rat's "self-healing" capacity 3 days after MCAO. And the "self-healing" capacity can be strengthen by acupuncture treatment through increasing cerebral blood flow, neurogenesis, and regulation of gene transcription or GSK-3β and PP2A expression. In conclusion, the present study indicates that the underlying mechanism of acupuncture treatment on neural functional damage caused by focal ischemia injury is a multiple interaction which may involve improved cerebral blood supply, neurogenesis, and regulation of gene transcription or GSK-3β and PP2A expression in MCAO rats.

No MeSH data available.


Related in: MedlinePlus