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Effect of Flos carthami Extract and α 1-Adrenergic Antagonists on the Porcine Proximal Ureteral Peristalsis.

Wu SY, Man KM, Shen JL, Chen HY, Chang CH, Tsai FJ, Hsieh WT, Winardi D, Lee YJ, Tsai KS, Lin YN, Chen YH, Chen WC - Evid Based Complement Alternat Med (2014)

Bottom Line: We previously performed a screening and confirmed the in vivo antilithic effect of FC extract.The results showed that doxazosin, tamsulosin, and terazosin dose (approximately 4.5 × 10(-6) - 4.5 × 10(-1) μg/mL) dependently inhibited both 10(-4) and 10(-3) M PE-induced ureteral peristalsis.FC extract achieved 6.2% ± 10.1%, 21.8% ± 6.8%, and 24.0% ± 5.6% inhibitions of 10(-4) M PE-induced peristalsis at doses of 5 × 10(3), 1 × 10(4), and 2 × 10(4) μg/mL, respectively, since FC extract was unable to completely inhibit PE-induced ureteral peristalsis, suggesting the antilithic effect of FC extract is related to mechanisms other than modulation of ureteral peristalsis.

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesiology, Tungs' Taichung MetroHarbor Hospital, Taichung 435, Taiwan ; Graduate Institute of Integrated Medicine, Institute of Chinese Medicine, School of Chinese Medicine, Department of Pharmacology, Research Center for Chinese Medicine and Acupuncture, China Medical University, Taichung 404, Taiwan.

ABSTRACT
Traditional Chinese medicine (TCM) has been proposed to prevent urolithiasis. In China, Flos carthami (FC, also known as Carthamus tinctorius) (Safflower; Chinese name: Hong Hua/) has been used to treat urological diseases for centuries. We previously performed a screening and confirmed the in vivo antilithic effect of FC extract. Here, ex vivo organ bath experiment was further performed to study the effect of FC extract on the inhibition of phenylepinephrine (PE) (10(-4) and 10(-3) M) ureteral peristalsis of porcine ureters with several α 1-adrenergic antagonists (doxazosin, tamsulosin, and terazosin) as experimental controls. The results showed that doxazosin, tamsulosin, and terazosin dose (approximately 4.5 × 10(-6) - 4.5 × 10(-1) μg/mL) dependently inhibited both 10(-4) and 10(-3) M PE-induced ureteral peristalsis. FC extract achieved 6.2% ± 10.1%, 21.8% ± 6.8%, and 24.0% ± 5.6% inhibitions of 10(-4) M PE-induced peristalsis at doses of 5 × 10(3), 1 × 10(4), and 2 × 10(4) μg/mL, respectively, since FC extract was unable to completely inhibit PE-induced ureteral peristalsis, suggesting the antilithic effect of FC extract is related to mechanisms other than modulation of ureteral peristalsis.

No MeSH data available.


Related in: MedlinePlus

Inhibitory effect of tamsulosin on porcine ureteral peristalsis. Graphic representation of concentration-response curves. The calculated data were presented as mean ± SEM for at least four different experiments. *P < 0.05 compared to control group.
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fig3: Inhibitory effect of tamsulosin on porcine ureteral peristalsis. Graphic representation of concentration-response curves. The calculated data were presented as mean ± SEM for at least four different experiments. *P < 0.05 compared to control group.

Mentions: A total of 100 ureteral samples were used in this study, and 88 were viable for the experiment. Ureteral peristalsis was recorded on polygraph paper. All tested α1-adrenoceptor antagonists exerted an inhibitory effect on PE-induced ureteral peristalsis. Doxazosin (Figure 2), tamsulosin (Figure 3), and terazosin (Figure 4) dose dependently inhibited both 10−4 and 10−3 M PE-induced ureteral peristalsis.


Effect of Flos carthami Extract and α 1-Adrenergic Antagonists on the Porcine Proximal Ureteral Peristalsis.

Wu SY, Man KM, Shen JL, Chen HY, Chang CH, Tsai FJ, Hsieh WT, Winardi D, Lee YJ, Tsai KS, Lin YN, Chen YH, Chen WC - Evid Based Complement Alternat Med (2014)

Inhibitory effect of tamsulosin on porcine ureteral peristalsis. Graphic representation of concentration-response curves. The calculated data were presented as mean ± SEM for at least four different experiments. *P < 0.05 compared to control group.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4120800&req=5

fig3: Inhibitory effect of tamsulosin on porcine ureteral peristalsis. Graphic representation of concentration-response curves. The calculated data were presented as mean ± SEM for at least four different experiments. *P < 0.05 compared to control group.
Mentions: A total of 100 ureteral samples were used in this study, and 88 were viable for the experiment. Ureteral peristalsis was recorded on polygraph paper. All tested α1-adrenoceptor antagonists exerted an inhibitory effect on PE-induced ureteral peristalsis. Doxazosin (Figure 2), tamsulosin (Figure 3), and terazosin (Figure 4) dose dependently inhibited both 10−4 and 10−3 M PE-induced ureteral peristalsis.

Bottom Line: We previously performed a screening and confirmed the in vivo antilithic effect of FC extract.The results showed that doxazosin, tamsulosin, and terazosin dose (approximately 4.5 × 10(-6) - 4.5 × 10(-1) μg/mL) dependently inhibited both 10(-4) and 10(-3) M PE-induced ureteral peristalsis.FC extract achieved 6.2% ± 10.1%, 21.8% ± 6.8%, and 24.0% ± 5.6% inhibitions of 10(-4) M PE-induced peristalsis at doses of 5 × 10(3), 1 × 10(4), and 2 × 10(4) μg/mL, respectively, since FC extract was unable to completely inhibit PE-induced ureteral peristalsis, suggesting the antilithic effect of FC extract is related to mechanisms other than modulation of ureteral peristalsis.

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesiology, Tungs' Taichung MetroHarbor Hospital, Taichung 435, Taiwan ; Graduate Institute of Integrated Medicine, Institute of Chinese Medicine, School of Chinese Medicine, Department of Pharmacology, Research Center for Chinese Medicine and Acupuncture, China Medical University, Taichung 404, Taiwan.

ABSTRACT
Traditional Chinese medicine (TCM) has been proposed to prevent urolithiasis. In China, Flos carthami (FC, also known as Carthamus tinctorius) (Safflower; Chinese name: Hong Hua/) has been used to treat urological diseases for centuries. We previously performed a screening and confirmed the in vivo antilithic effect of FC extract. Here, ex vivo organ bath experiment was further performed to study the effect of FC extract on the inhibition of phenylepinephrine (PE) (10(-4) and 10(-3) M) ureteral peristalsis of porcine ureters with several α 1-adrenergic antagonists (doxazosin, tamsulosin, and terazosin) as experimental controls. The results showed that doxazosin, tamsulosin, and terazosin dose (approximately 4.5 × 10(-6) - 4.5 × 10(-1) μg/mL) dependently inhibited both 10(-4) and 10(-3) M PE-induced ureteral peristalsis. FC extract achieved 6.2% ± 10.1%, 21.8% ± 6.8%, and 24.0% ± 5.6% inhibitions of 10(-4) M PE-induced peristalsis at doses of 5 × 10(3), 1 × 10(4), and 2 × 10(4) μg/mL, respectively, since FC extract was unable to completely inhibit PE-induced ureteral peristalsis, suggesting the antilithic effect of FC extract is related to mechanisms other than modulation of ureteral peristalsis.

No MeSH data available.


Related in: MedlinePlus