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Sequential Statistical Optimization of Media Components for the Production of Glucoamylase by Thermophilic Fungus Humicola grisea MTCC 352.

Ramesh V, Ramachandra Murty V - Enzyme Res (2014)

Bottom Line: Steepest ascent method was performed to identify the location of optimum.The experimental activity of 12.27 U/mL obtained was close to the predicted activity of 12.15.The glucoamylase production was found to increase from 4.57 U/mL to 12.27 U/mL, a 2.68-fold enhancement, as compared to the unoptimized medium.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology, Manipal Institute of Technology, Manipal University, Manipal, Karnataka 576104, India.

ABSTRACT
Glucoamylase is an industrially important enzyme which converts soluble starch into glucose. The media components for the production of glucoamylase from thermophilic fungus Humicola grisea MTCC 352 have been optimized. Eight media components, namely, soluble starch, yeast extract, KH2PO4, K2HPO4, NaCl, CaCl2, MgSO4 ·7H2O, and Vogel's trace elements solution, were first screened for their effect on the production of glucoamylase and only four components (soluble starch, yeast extract, K2HPO4, and MgSO4 ·7H2O) were identified as statistically significant using Plackett-Burman design. It was fitted into a first-order model (R (2) = 0.9859). Steepest ascent method was performed to identify the location of optimum. Central composite design was employed to determine the optimum values (soluble starch: 28.41 g/L, yeast extract: 9.61 g/L, K2HPO4: 2.42 g/L, and MgSO4 ·7H2O: 1.91 g/L). The experimental activity of 12.27 U/mL obtained was close to the predicted activity of 12.15. High R (2) value (0.9397), low PRESS value (9.47), and AARD values (2.07%) indicate the accuracy of the proposed model. The glucoamylase production was found to increase from 4.57 U/mL to 12.27 U/mL, a 2.68-fold enhancement, as compared to the unoptimized medium.

No MeSH data available.


Related in: MedlinePlus

Pareto plot shows the effect of media on glucoamylase activity.
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Related In: Results  -  Collection


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fig1: Pareto plot shows the effect of media on glucoamylase activity.

Mentions: The same phenomena were explained graphically using Pareto chart (Figure 1). It explains the importance of the individual main effect of each factor to determine whether they are significantly different from zero. These values are represented by horizontal columns in the Pareto chart. For a 95% confidence level and three degrees of freedom, the t value equals 3.18 and is shown in the plot as a vertical line. This indicates the minimum statistically significant effect for 95% confidence level. It is clear from the Pareto chart that the four factors A, B, D, and G are significant, and therefore these four factors were taken up for further studies.


Sequential Statistical Optimization of Media Components for the Production of Glucoamylase by Thermophilic Fungus Humicola grisea MTCC 352.

Ramesh V, Ramachandra Murty V - Enzyme Res (2014)

Pareto plot shows the effect of media on glucoamylase activity.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4120784&req=5

fig1: Pareto plot shows the effect of media on glucoamylase activity.
Mentions: The same phenomena were explained graphically using Pareto chart (Figure 1). It explains the importance of the individual main effect of each factor to determine whether they are significantly different from zero. These values are represented by horizontal columns in the Pareto chart. For a 95% confidence level and three degrees of freedom, the t value equals 3.18 and is shown in the plot as a vertical line. This indicates the minimum statistically significant effect for 95% confidence level. It is clear from the Pareto chart that the four factors A, B, D, and G are significant, and therefore these four factors were taken up for further studies.

Bottom Line: Steepest ascent method was performed to identify the location of optimum.The experimental activity of 12.27 U/mL obtained was close to the predicted activity of 12.15.The glucoamylase production was found to increase from 4.57 U/mL to 12.27 U/mL, a 2.68-fold enhancement, as compared to the unoptimized medium.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology, Manipal Institute of Technology, Manipal University, Manipal, Karnataka 576104, India.

ABSTRACT
Glucoamylase is an industrially important enzyme which converts soluble starch into glucose. The media components for the production of glucoamylase from thermophilic fungus Humicola grisea MTCC 352 have been optimized. Eight media components, namely, soluble starch, yeast extract, KH2PO4, K2HPO4, NaCl, CaCl2, MgSO4 ·7H2O, and Vogel's trace elements solution, were first screened for their effect on the production of glucoamylase and only four components (soluble starch, yeast extract, K2HPO4, and MgSO4 ·7H2O) were identified as statistically significant using Plackett-Burman design. It was fitted into a first-order model (R (2) = 0.9859). Steepest ascent method was performed to identify the location of optimum. Central composite design was employed to determine the optimum values (soluble starch: 28.41 g/L, yeast extract: 9.61 g/L, K2HPO4: 2.42 g/L, and MgSO4 ·7H2O: 1.91 g/L). The experimental activity of 12.27 U/mL obtained was close to the predicted activity of 12.15. High R (2) value (0.9397), low PRESS value (9.47), and AARD values (2.07%) indicate the accuracy of the proposed model. The glucoamylase production was found to increase from 4.57 U/mL to 12.27 U/mL, a 2.68-fold enhancement, as compared to the unoptimized medium.

No MeSH data available.


Related in: MedlinePlus