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Mapping and genotypic analysis of the NK-lysin gene in chicken.

Lee MO, Yang E, Morisson M, Vignal A, Huang YZ, Cheng HH, Muir WM, Lamont SJ, Lillehoj HS, Lee SH, Womack JE - Genet. Sel. Evol. (2014)

Bottom Line: Previously, we reported a functionally significant SNP at position 271 of the NK-lysin coding sequence in two different chicken breeds.Here, we examined this SNP and found that the A allele appears to be more common than the G allele in these heritage breeds and inbred lines.SNP analyses revealed that the A allele, which encodes a peptide with a higher antimicrobial activity, is more common than the G allele in our tested inbred lines and heritage breeds.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Veterinary Pathobiology, Texas A & M University, College Station, TX 77843, USA. jwomack@cvm.tamu.edu.

ABSTRACT

Background: Antimicrobial peptides (AMP) are important elements of the first line of defence against pathogens in animals. NK-lysin is a cationic AMP that plays a critical role in innate immunity. The chicken NK-lysin gene has been cloned and its antimicrobial and anticancer activity has been described but its location in the chicken genome remains unknown. Here, we mapped the NK-lysin gene and examined the distribution of a functionally significant single nucleotide polymorphism (SNP) among different chicken inbred lines and heritage breeds.

Results: A 6000 rad radiation hybrid panel (ChickRH6) was used to map the NK-lysin gene to the distal end of chromosome 22. Two additional genes, the adipocyte enhancer-binding protein 1-like gene (AEBP1) and the DNA polymerase delta subunit 2-like (POLD2) gene, are located in the same NW_003779909 contig as NK-lysin, and were thus indirectly mapped to chromosome 22 as well. Previously, we reported a functionally significant SNP at position 271 of the NK-lysin coding sequence in two different chicken breeds. Here, we examined this SNP and found that the A allele appears to be more common than the G allele in these heritage breeds and inbred lines.

Conclusions: The chicken NK-lysin gene mapped to the distal end of chromosome 22. Two additional genes, AEBP1 and POLD2, were indirectly mapped to chromosome 22 also. SNP analyses revealed that the A allele, which encodes a peptide with a higher antimicrobial activity, is more common than the G allele in our tested inbred lines and heritage breeds.

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Radiation hybrid mapping of the NK-lysin gene and other markers on chromosome GGA22. The GGA22 RH map (left) is 352 cR long and is aligned to the chicken genome assembly (right); the location of the NK-lysin gene is indicated at the distal end of chromosome GGA22.
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Figure 2: Radiation hybrid mapping of the NK-lysin gene and other markers on chromosome GGA22. The GGA22 RH map (left) is 352 cR long and is aligned to the chicken genome assembly (right); the location of the NK-lysin gene is indicated at the distal end of chromosome GGA22.

Mentions: Using two-point analysis, the NK-lysin gene was included in a linkage group of 104 markers corresponding to microchromosome GGA22. After multipoint analysis, the framework map was composed of 23 markers covering 351.8 cR. Three additional markers AEBP1, LRRTM4 (leucine-rich repeat transmembrane neuronal 4) and ADRA1A (adrenoceptor alpha 1A) were integrated at their best possible locations on the comprehensive map (Figure 2).


Mapping and genotypic analysis of the NK-lysin gene in chicken.

Lee MO, Yang E, Morisson M, Vignal A, Huang YZ, Cheng HH, Muir WM, Lamont SJ, Lillehoj HS, Lee SH, Womack JE - Genet. Sel. Evol. (2014)

Radiation hybrid mapping of the NK-lysin gene and other markers on chromosome GGA22. The GGA22 RH map (left) is 352 cR long and is aligned to the chicken genome assembly (right); the location of the NK-lysin gene is indicated at the distal end of chromosome GGA22.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4120735&req=5

Figure 2: Radiation hybrid mapping of the NK-lysin gene and other markers on chromosome GGA22. The GGA22 RH map (left) is 352 cR long and is aligned to the chicken genome assembly (right); the location of the NK-lysin gene is indicated at the distal end of chromosome GGA22.
Mentions: Using two-point analysis, the NK-lysin gene was included in a linkage group of 104 markers corresponding to microchromosome GGA22. After multipoint analysis, the framework map was composed of 23 markers covering 351.8 cR. Three additional markers AEBP1, LRRTM4 (leucine-rich repeat transmembrane neuronal 4) and ADRA1A (adrenoceptor alpha 1A) were integrated at their best possible locations on the comprehensive map (Figure 2).

Bottom Line: Previously, we reported a functionally significant SNP at position 271 of the NK-lysin coding sequence in two different chicken breeds.Here, we examined this SNP and found that the A allele appears to be more common than the G allele in these heritage breeds and inbred lines.SNP analyses revealed that the A allele, which encodes a peptide with a higher antimicrobial activity, is more common than the G allele in our tested inbred lines and heritage breeds.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Veterinary Pathobiology, Texas A & M University, College Station, TX 77843, USA. jwomack@cvm.tamu.edu.

ABSTRACT

Background: Antimicrobial peptides (AMP) are important elements of the first line of defence against pathogens in animals. NK-lysin is a cationic AMP that plays a critical role in innate immunity. The chicken NK-lysin gene has been cloned and its antimicrobial and anticancer activity has been described but its location in the chicken genome remains unknown. Here, we mapped the NK-lysin gene and examined the distribution of a functionally significant single nucleotide polymorphism (SNP) among different chicken inbred lines and heritage breeds.

Results: A 6000 rad radiation hybrid panel (ChickRH6) was used to map the NK-lysin gene to the distal end of chromosome 22. Two additional genes, the adipocyte enhancer-binding protein 1-like gene (AEBP1) and the DNA polymerase delta subunit 2-like (POLD2) gene, are located in the same NW_003779909 contig as NK-lysin, and were thus indirectly mapped to chromosome 22 as well. Previously, we reported a functionally significant SNP at position 271 of the NK-lysin coding sequence in two different chicken breeds. Here, we examined this SNP and found that the A allele appears to be more common than the G allele in these heritage breeds and inbred lines.

Conclusions: The chicken NK-lysin gene mapped to the distal end of chromosome 22. Two additional genes, AEBP1 and POLD2, were indirectly mapped to chromosome 22 also. SNP analyses revealed that the A allele, which encodes a peptide with a higher antimicrobial activity, is more common than the G allele in our tested inbred lines and heritage breeds.

Show MeSH