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The Drosophila mojavensis Bari3 transposon: distribution and functional characterization.

Palazzo A, Moschetti R, Caizzi R, Marsano RM - Mob DNA (2014)

Bottom Line: The results suggest that Bari3 is transposition-competent.Our results strongly suggest that Bari3 is an independent element that has generated genomic diversity in D. mojavensis.These results will aid the studies on the Bari family of transposons, which is intriguing for its widespread diffusion in Drosophilids coupled with a structural diversity generated during the evolution of Bari-like elements in their host genomes.

View Article: PubMed Central - HTML - PubMed

Affiliation: Dipartimento di Biologia, Università degli Studi di Bari "Aldo Moro", Via Orabona 4, 70125 Bari, Italy.

ABSTRACT

Background: Bari-like transposons belong to the Tc1-mariner superfamily, and they have been identified in several genomes of the Drosophila genus. This transposon's family has been used as paradigm to investigate the complex dynamics underlying the persistence and structural evolution of transposable elements (TEs) within a genome. Three structural Bari variants have been identified so far and can be distinguished based on the organization of their terminal inverted repeats. Bari3 is the last discovered member of this family identified in Drosophila mojavensis, a recently emerged species of the Repleta group of the genus Drosophila.

Results: We studied the insertion pattern of Bari3 in different D. mojavensis populations and found evidence of recent transposition activity. Analysis of the transposase domains unveiled the presence of a functional nuclear localization signal, as well as a functional binding domain. Using luciferase-based assays, we investigated the promoter activity of Bari3 as well as the interaction of its transposase with its left terminus. The results suggest that Bari3 is transposition-competent. Finally we demonstrated transposase transcript processing when the transposase gene is overexpressed in vivo and in vitro.

Conclusions: Bari3 displays very similar structural and functional features with its close relative, Bari1. Our results strongly suggest that Bari3 is an independent element that has generated genomic diversity in D. mojavensis. It can autonomously transcribe its transposase gene, which in turn can localize in the nucleus and bind the terminal inverted repeats of the transposon. Nevertheless, the identification of an unpredicted spliced form of the Bari3 transposase transcript allows us to hypothesize a control mechanism of its mobility based on mRNA processing. These results will aid the studies on the Bari family of transposons, which is intriguing for its widespread diffusion in Drosophilids coupled with a structural diversity generated during the evolution of Bari-like elements in their host genomes.

No MeSH data available.


Related in: MedlinePlus

Geographical origin of the Drosophila mojavensis strains analyzed in this study. The prefix 15081 has been omitted for space restriction (see Table 1). D. mojavensis subspecies are indicated according to the color code showed.
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Figure 1: Geographical origin of the Drosophila mojavensis strains analyzed in this study. The prefix 15081 has been omitted for space restriction (see Table 1). D. mojavensis subspecies are indicated according to the color code showed.

Mentions: In order to estimate the activity of the Bari3, we analyzed its distribution in the population of D. mojavensis collected in different geographical regions of California and Mexico (Figure 1 and Table 1).


The Drosophila mojavensis Bari3 transposon: distribution and functional characterization.

Palazzo A, Moschetti R, Caizzi R, Marsano RM - Mob DNA (2014)

Geographical origin of the Drosophila mojavensis strains analyzed in this study. The prefix 15081 has been omitted for space restriction (see Table 1). D. mojavensis subspecies are indicated according to the color code showed.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4120734&req=5

Figure 1: Geographical origin of the Drosophila mojavensis strains analyzed in this study. The prefix 15081 has been omitted for space restriction (see Table 1). D. mojavensis subspecies are indicated according to the color code showed.
Mentions: In order to estimate the activity of the Bari3, we analyzed its distribution in the population of D. mojavensis collected in different geographical regions of California and Mexico (Figure 1 and Table 1).

Bottom Line: The results suggest that Bari3 is transposition-competent.Our results strongly suggest that Bari3 is an independent element that has generated genomic diversity in D. mojavensis.These results will aid the studies on the Bari family of transposons, which is intriguing for its widespread diffusion in Drosophilids coupled with a structural diversity generated during the evolution of Bari-like elements in their host genomes.

View Article: PubMed Central - HTML - PubMed

Affiliation: Dipartimento di Biologia, Università degli Studi di Bari "Aldo Moro", Via Orabona 4, 70125 Bari, Italy.

ABSTRACT

Background: Bari-like transposons belong to the Tc1-mariner superfamily, and they have been identified in several genomes of the Drosophila genus. This transposon's family has been used as paradigm to investigate the complex dynamics underlying the persistence and structural evolution of transposable elements (TEs) within a genome. Three structural Bari variants have been identified so far and can be distinguished based on the organization of their terminal inverted repeats. Bari3 is the last discovered member of this family identified in Drosophila mojavensis, a recently emerged species of the Repleta group of the genus Drosophila.

Results: We studied the insertion pattern of Bari3 in different D. mojavensis populations and found evidence of recent transposition activity. Analysis of the transposase domains unveiled the presence of a functional nuclear localization signal, as well as a functional binding domain. Using luciferase-based assays, we investigated the promoter activity of Bari3 as well as the interaction of its transposase with its left terminus. The results suggest that Bari3 is transposition-competent. Finally we demonstrated transposase transcript processing when the transposase gene is overexpressed in vivo and in vitro.

Conclusions: Bari3 displays very similar structural and functional features with its close relative, Bari1. Our results strongly suggest that Bari3 is an independent element that has generated genomic diversity in D. mojavensis. It can autonomously transcribe its transposase gene, which in turn can localize in the nucleus and bind the terminal inverted repeats of the transposon. Nevertheless, the identification of an unpredicted spliced form of the Bari3 transposase transcript allows us to hypothesize a control mechanism of its mobility based on mRNA processing. These results will aid the studies on the Bari family of transposons, which is intriguing for its widespread diffusion in Drosophilids coupled with a structural diversity generated during the evolution of Bari-like elements in their host genomes.

No MeSH data available.


Related in: MedlinePlus