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Xilei san ameliorates experimental colitis in rats by selectively degrading proinflammatory mediators and promoting mucosal repair.

Hao Y, Nagase K, Hori K, Wang S, Kogure Y, Fukunaga K, Kashiwamura S, Yamamoto S, Nakamura S, Li J, Miwa H, Noguchi K, Dai Y - Evid Based Complement Alternat Med (2014)

Bottom Line: Intrarectal administration of XLS attenuated the DSS-induced colitis, as evidenced by a reduction in both the histological damage score and myeloperoxidase activity.It also decreased the levels of proinflammatory cytokines, but increased the mucosal repair-related cytokines.These results suggest that XLS attenuates DSS-induced colitis by degrading proinflammatory mediators and promoting mucosal repair.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacy, School of Pharmacy, Hyogo University of Health Sciences, 1-3-6 Minatojima, Chuo-ku, Kobe, Hyogo 650-8530, Japan ; Division of Gastroenterology, Department of Internal Medicine, Hyogo College of Medicine, Nishinomiya, Hyogo 663-8501, Japan.

ABSTRACT
Xilei san (XLS), a herbal preparation widely used in China for erosive and ulcerative diseases, has been shown to be effective in ulcerative colitis (UC). The present experiments were conducted to assess its efficacy and determine its mechanism of action in a rat model that resembles human UC. The model was induced by adding 4% dextran sulfate sodium (DSS) to the rats' drinking water for 7 days. XLS was administered daily by retention enema from day 2 to day 7; the rats were sacrificed on day 8. The colon tissues were obtained for further experiments. A histological damage score and the activity of tissue myeloperoxidase were used to evaluate the severity of the colitis. The colonic cytokine levels were detected in a suspension array, and epithelial proliferation was assessed using Ki-67 immunohistochemistry. Intrarectal administration of XLS attenuated the DSS-induced colitis, as evidenced by a reduction in both the histological damage score and myeloperoxidase activity. It also decreased the levels of proinflammatory cytokines, but increased the mucosal repair-related cytokines. In addition, the epithelial Ki-67 expression was upregulated by XLS. These results suggest that XLS attenuates DSS-induced colitis by degrading proinflammatory mediators and promoting mucosal repair. XLS could be a potential topical treatment for human UC.

No MeSH data available.


Related in: MedlinePlus

In rats, XLS inhibited the increase of the colonic chemokines (a) GRO/KC and (b) MCP-1 induced by DSS. Numbers in brackets represent the sample numbers of each group. **P < 0.01 versus water + saline, ##P < 0.01 versus DSS + Saline.
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fig4: In rats, XLS inhibited the increase of the colonic chemokines (a) GRO/KC and (b) MCP-1 induced by DSS. Numbers in brackets represent the sample numbers of each group. **P < 0.01 versus water + saline, ##P < 0.01 versus DSS + Saline.

Mentions: In addition to the differences in the concentration of a proinflammatory cytokine IL-1β, two colonic chemokines—GRO/KC (Figure 4(a)) and MCP-1 (Figure 4(b))—were significantly greater in the DSS + saline group than in the water + saline group (817.92 ± 308.07 pg/mg protein [N = 9] versus 84.99 ± 4.73 [N = 10], P < 0.01 for GRO/KC, and 2065.03 ± 316.12 pg/mg protein [N = 9] versus 391.63 ± 59.02 [N = 10], P < 0.01 for MCP-1, resp.). Both GRO/KC and MCP-1 protein levels were suppressed by XLS, as seen when comparing the two groups, DSS + XLS, and DSS + saline (155.31 ± 15.38 pg/mg protein [N = 10] versus 817.92 ± 308.07 [N = 9], P < 0.01 for GRO/KC, and 666.11 ± 45.73 pg/mg protein [N = 10] versus 2065.03 ± 316.12 [N = 9], P < 0.01 for MCP-1, resp.).


Xilei san ameliorates experimental colitis in rats by selectively degrading proinflammatory mediators and promoting mucosal repair.

Hao Y, Nagase K, Hori K, Wang S, Kogure Y, Fukunaga K, Kashiwamura S, Yamamoto S, Nakamura S, Li J, Miwa H, Noguchi K, Dai Y - Evid Based Complement Alternat Med (2014)

In rats, XLS inhibited the increase of the colonic chemokines (a) GRO/KC and (b) MCP-1 induced by DSS. Numbers in brackets represent the sample numbers of each group. **P < 0.01 versus water + saline, ##P < 0.01 versus DSS + Saline.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4120479&req=5

fig4: In rats, XLS inhibited the increase of the colonic chemokines (a) GRO/KC and (b) MCP-1 induced by DSS. Numbers in brackets represent the sample numbers of each group. **P < 0.01 versus water + saline, ##P < 0.01 versus DSS + Saline.
Mentions: In addition to the differences in the concentration of a proinflammatory cytokine IL-1β, two colonic chemokines—GRO/KC (Figure 4(a)) and MCP-1 (Figure 4(b))—were significantly greater in the DSS + saline group than in the water + saline group (817.92 ± 308.07 pg/mg protein [N = 9] versus 84.99 ± 4.73 [N = 10], P < 0.01 for GRO/KC, and 2065.03 ± 316.12 pg/mg protein [N = 9] versus 391.63 ± 59.02 [N = 10], P < 0.01 for MCP-1, resp.). Both GRO/KC and MCP-1 protein levels were suppressed by XLS, as seen when comparing the two groups, DSS + XLS, and DSS + saline (155.31 ± 15.38 pg/mg protein [N = 10] versus 817.92 ± 308.07 [N = 9], P < 0.01 for GRO/KC, and 666.11 ± 45.73 pg/mg protein [N = 10] versus 2065.03 ± 316.12 [N = 9], P < 0.01 for MCP-1, resp.).

Bottom Line: Intrarectal administration of XLS attenuated the DSS-induced colitis, as evidenced by a reduction in both the histological damage score and myeloperoxidase activity.It also decreased the levels of proinflammatory cytokines, but increased the mucosal repair-related cytokines.These results suggest that XLS attenuates DSS-induced colitis by degrading proinflammatory mediators and promoting mucosal repair.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacy, School of Pharmacy, Hyogo University of Health Sciences, 1-3-6 Minatojima, Chuo-ku, Kobe, Hyogo 650-8530, Japan ; Division of Gastroenterology, Department of Internal Medicine, Hyogo College of Medicine, Nishinomiya, Hyogo 663-8501, Japan.

ABSTRACT
Xilei san (XLS), a herbal preparation widely used in China for erosive and ulcerative diseases, has been shown to be effective in ulcerative colitis (UC). The present experiments were conducted to assess its efficacy and determine its mechanism of action in a rat model that resembles human UC. The model was induced by adding 4% dextran sulfate sodium (DSS) to the rats' drinking water for 7 days. XLS was administered daily by retention enema from day 2 to day 7; the rats were sacrificed on day 8. The colon tissues were obtained for further experiments. A histological damage score and the activity of tissue myeloperoxidase were used to evaluate the severity of the colitis. The colonic cytokine levels were detected in a suspension array, and epithelial proliferation was assessed using Ki-67 immunohistochemistry. Intrarectal administration of XLS attenuated the DSS-induced colitis, as evidenced by a reduction in both the histological damage score and myeloperoxidase activity. It also decreased the levels of proinflammatory cytokines, but increased the mucosal repair-related cytokines. In addition, the epithelial Ki-67 expression was upregulated by XLS. These results suggest that XLS attenuates DSS-induced colitis by degrading proinflammatory mediators and promoting mucosal repair. XLS could be a potential topical treatment for human UC.

No MeSH data available.


Related in: MedlinePlus