Limits...
FluxTransgenics: a flexible LIMS-based tool for management of plant transformation experimental data

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ABSTRACT

Background: The production and commercial release of Genetically Modified Organisms (GMOs) are currently the focus of important discussions. In order to guarantee the quality and reliability of their trials, companies and institutions working on this subject must adopt new approaches on management, organization and recording of laboratory conditions where field studies are performed. Computational systems for management and storage of laboratory data known as Laboratory Information Management Systems (LIMS) are essential tools to achieve this.

Results: In this work, we have used the SIGLa system – a workflow based LIMS as a framework to develop the FluxTransgenics system for a GMOs laboratory of Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Maize and Sorghum (Sete Lagoas, MG - Brazil). A workflow representing all stages of the transgenic maize plants generation has been developed and uploaded in FluxTransgenics. This workflow models the activities involved in maize and sorghum transformation using the Agrobacterium tumefaciens method. By uploading this workflow in the SIGLa system we have created Fluxtransgenics, a complete LIMS for managing plant transformation data.

Conclusions: FluxTransgenics presents a solution for the management of the data produced by a laboratory of genetically modified plants that is efficient and supports different kinds of information. Its adoption will contribute to guarantee the quality of activities and products in the process of transgenic production and enforce the use of Good Laboratory Practices (GLP).

Conclusions: The adoption of the transformation protocol associated to the use of FluxTransgenics has made it possible to increase productivity by at least 300%, increasing the efficiency of the experiments from between 0.5 and 1 percent to about 3%. This has been achieved by an increase in the number of experiments performed and a more accurate choice of parameters, all of which have been made possible because it became easier to identify which were the most promising next steps of the experiments. The FluxTransgenics system is available for use by other laboratories, and the workflows that have been developed can be adapted to other contexts.

No MeSH data available.


Related in: MedlinePlus

Agrobacterium tumefaciens’s  Transformation Workflow . Workflow of the maize and sorghum transformation process by Agrobacterium tumefaciens. Each box represents an activity in the process and the arrow from an activity to the other represents a transition.
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Figure 2: Agrobacterium tumefaciens’s Transformation Workflow . Workflow of the maize and sorghum transformation process by Agrobacterium tumefaciens. Each box represents an activity in the process and the arrow from an activity to the other represents a transition.

Mentions: The workflow has been constructed considering all steps of a transformation method, as shown in Figure 2. The protocol used for Agrobacterium-mediated transformation of immature zygotic embryos is based on the work of Frame et al.[24] and Vega et al.[25]. For details on the protocol used, please see the supplementary material available at the address http://www.luar.dcc.ufmg.br/FluxTransgenics.


FluxTransgenics: a flexible LIMS-based tool for management of plant transformation experimental data
Agrobacterium tumefaciens’s  Transformation Workflow . Workflow of the maize and sorghum transformation process by Agrobacterium tumefaciens. Each box represents an activity in the process and the arrow from an activity to the other represents a transition.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4119470&req=5

Figure 2: Agrobacterium tumefaciens’s Transformation Workflow . Workflow of the maize and sorghum transformation process by Agrobacterium tumefaciens. Each box represents an activity in the process and the arrow from an activity to the other represents a transition.
Mentions: The workflow has been constructed considering all steps of a transformation method, as shown in Figure 2. The protocol used for Agrobacterium-mediated transformation of immature zygotic embryos is based on the work of Frame et al.[24] and Vega et al.[25]. For details on the protocol used, please see the supplementary material available at the address http://www.luar.dcc.ufmg.br/FluxTransgenics.

View Article: PubMed Central - HTML

ABSTRACT

Background: The production and commercial release of Genetically Modified Organisms (GMOs) are currently the focus of important discussions. In order to guarantee the quality and reliability of their trials, companies and institutions working on this subject must adopt new approaches on management, organization and recording of laboratory conditions where field studies are performed. Computational systems for management and storage of laboratory data known as Laboratory Information Management Systems (LIMS) are essential tools to achieve this.

Results: In this work, we have used the SIGLa system – a workflow based LIMS as a framework to develop the FluxTransgenics system for a GMOs laboratory of Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Maize and Sorghum (Sete Lagoas, MG - Brazil). A workflow representing all stages of the transgenic maize plants generation has been developed and uploaded in FluxTransgenics. This workflow models the activities involved in maize and sorghum transformation using the Agrobacterium tumefaciens method. By uploading this workflow in the SIGLa system we have created Fluxtransgenics, a complete LIMS for managing plant transformation data.

Conclusions: FluxTransgenics presents a solution for the management of the data produced by a laboratory of genetically modified plants that is efficient and supports different kinds of information. Its adoption will contribute to guarantee the quality of activities and products in the process of transgenic production and enforce the use of Good Laboratory Practices (GLP).

Conclusions: The adoption of the transformation protocol associated to the use of FluxTransgenics has made it possible to increase productivity by at least 300%, increasing the efficiency of the experiments from between 0.5 and 1 percent to about 3%. This has been achieved by an increase in the number of experiments performed and a more accurate choice of parameters, all of which have been made possible because it became easier to identify which were the most promising next steps of the experiments. The FluxTransgenics system is available for use by other laboratories, and the workflows that have been developed can be adapted to other contexts.

No MeSH data available.


Related in: MedlinePlus