Reprogramming to a pluripotent state modifies mesenchymal stem cell resistance to oxidative stress.
Bottom Line: Catalase and superoxide dismutase activities were reduced in mb-iPSC and H9 when compared with mbMSC.Finally, cell adhesion under OS conditions was impaired in mb-iPSC when compared with mbMSC, albeit similar to H9.Thus, reprogramming leads to profound modifications in extracellular ROS production accompanied by loss of the ability to handle OS.
Affiliation: Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.Show MeSH
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Mentions: Finally, we decided to test if the higher susceptibility of pluripotent stem cells to ROS would result in an impaired adhesion capability compared with mbMSC. Cells were plated with different concentrations of H2O2 and adhesion was estimated after 2 hrs. At 200 μM of H2O2, adhesion of H9 and mb-iPSC was significantly impaired, while mbMSC maintained adhesion in similar levels to control. Above 400 μM, adhesion was compromised in all cell types, even though the percentages of reduction were more pronounced in pluripotent stem cells (Fig. 5).
Affiliation: Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.