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Reprogramming to a pluripotent state modifies mesenchymal stem cell resistance to oxidative stress.

Asensi KD, Fortunato RS, dos Santos DS, Pacheco TS, de Rezende DF, Rodrigues DC, Mesquita FC, Kasai-Brunswick TH, de Carvalho AC, Carvalho DP, Carvalho AB, Goldenberg RC - J. Cell. Mol. Med. (2014)

Bottom Line: Catalase and superoxide dismutase activities were reduced in mb-iPSC and H9 when compared with mbMSC.Finally, cell adhesion under OS conditions was impaired in mb-iPSC when compared with mbMSC, albeit similar to H9.Thus, reprogramming leads to profound modifications in extracellular ROS production accompanied by loss of the ability to handle OS.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.

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Related in: MedlinePlus

Cell adhesion under oxidative stress. Cells were dissociated and replated under increasing concentrations of H2O2. There was a significant reduction in adhesion in (B) H9 (n = 6) and (C) mb-iPSC (n = 6) at 200 μM of H2O2 (*P < 0.05 compared with control), while (A) mbMSC (n = 6) adhesion remained similar to control levels. At 400 and 800 μM of H2O2, all cell types presented reductions in adhesion (***P < 0.001 compared with control); however, the percentage of reduction was more pronounced in pluripotent stem cells.
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fig05: Cell adhesion under oxidative stress. Cells were dissociated and replated under increasing concentrations of H2O2. There was a significant reduction in adhesion in (B) H9 (n = 6) and (C) mb-iPSC (n = 6) at 200 μM of H2O2 (*P < 0.05 compared with control), while (A) mbMSC (n = 6) adhesion remained similar to control levels. At 400 and 800 μM of H2O2, all cell types presented reductions in adhesion (***P < 0.001 compared with control); however, the percentage of reduction was more pronounced in pluripotent stem cells.

Mentions: Finally, we decided to test if the higher susceptibility of pluripotent stem cells to ROS would result in an impaired adhesion capability compared with mbMSC. Cells were plated with different concentrations of H2O2 and adhesion was estimated after 2 hrs. At 200 μM of H2O2, adhesion of H9 and mb-iPSC was significantly impaired, while mbMSC maintained adhesion in similar levels to control. Above 400 μM, adhesion was compromised in all cell types, even though the percentages of reduction were more pronounced in pluripotent stem cells (Fig. 5).


Reprogramming to a pluripotent state modifies mesenchymal stem cell resistance to oxidative stress.

Asensi KD, Fortunato RS, dos Santos DS, Pacheco TS, de Rezende DF, Rodrigues DC, Mesquita FC, Kasai-Brunswick TH, de Carvalho AC, Carvalho DP, Carvalho AB, Goldenberg RC - J. Cell. Mol. Med. (2014)

Cell adhesion under oxidative stress. Cells were dissociated and replated under increasing concentrations of H2O2. There was a significant reduction in adhesion in (B) H9 (n = 6) and (C) mb-iPSC (n = 6) at 200 μM of H2O2 (*P < 0.05 compared with control), while (A) mbMSC (n = 6) adhesion remained similar to control levels. At 400 and 800 μM of H2O2, all cell types presented reductions in adhesion (***P < 0.001 compared with control); however, the percentage of reduction was more pronounced in pluripotent stem cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4119388&req=5

fig05: Cell adhesion under oxidative stress. Cells were dissociated and replated under increasing concentrations of H2O2. There was a significant reduction in adhesion in (B) H9 (n = 6) and (C) mb-iPSC (n = 6) at 200 μM of H2O2 (*P < 0.05 compared with control), while (A) mbMSC (n = 6) adhesion remained similar to control levels. At 400 and 800 μM of H2O2, all cell types presented reductions in adhesion (***P < 0.001 compared with control); however, the percentage of reduction was more pronounced in pluripotent stem cells.
Mentions: Finally, we decided to test if the higher susceptibility of pluripotent stem cells to ROS would result in an impaired adhesion capability compared with mbMSC. Cells were plated with different concentrations of H2O2 and adhesion was estimated after 2 hrs. At 200 μM of H2O2, adhesion of H9 and mb-iPSC was significantly impaired, while mbMSC maintained adhesion in similar levels to control. Above 400 μM, adhesion was compromised in all cell types, even though the percentages of reduction were more pronounced in pluripotent stem cells (Fig. 5).

Bottom Line: Catalase and superoxide dismutase activities were reduced in mb-iPSC and H9 when compared with mbMSC.Finally, cell adhesion under OS conditions was impaired in mb-iPSC when compared with mbMSC, albeit similar to H9.Thus, reprogramming leads to profound modifications in extracellular ROS production accompanied by loss of the ability to handle OS.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.

Show MeSH
Related in: MedlinePlus