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Intramyocardial transplantation of cardiac telocytes decreases myocardial infarction and improves post-infarcted cardiac function in rats.

Zhao B, Liao Z, Chen S, Yuan Z, Yilin C, Lee KK, Qi X, Shen X, Zheng X, Quinn T, Cai D - J. Cell. Mol. Med. (2014)

Bottom Line: The cellular mechanism behind this beneficial effect on MI was partially attributed to increased cardiac angiogenesis, improved reconstruction of the CT network and decreased myocardial fibrosis.These combined effects decreased the infarct size, improved the reconstruction of the LV and enhanced myocardial function in MI.Our findings suggest that CTs could be considered as a potential cell source for therapeutic use to improve cardiac repair and function following MI, used either alone or in tandem with stem cells.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory for Regenerative Medicine, Ministry of Education, Ji Nan University, Guangzhou, China; Joint Laboratory for Regenerative Medicine, Chinese University of Hong Kong-Ji Nan University, Guangzhou, China; International Base of Collaboration for Science and Technology (JNU), The Ministry of Science and Technology & Guangdong Province, Guangzhou, China; Department of Developmental and Regenerative Biology, Ji Nan University, Guangzhou, China.

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Transplantation of cardiac telocytes enhanced angiogenesis in myocardial infarction (MI). The density of vessels in MI hearts 14-week post-LAD ligation was determined by comparing the presence of vWF+ vessels in the infarct zone and border zone. (I) In the infarcted area, the vessel density of the CT-treated group was significantly larger than the PBS-treated group (P < 0.05). The vessel density of the c-kit− cell-treated group appeared larger than the PBS-treated group, but was not statistically significant (P > 0.05). (A) Showing the semi-quantification analysis of vessel density. The representative morphologies of the PBS-treated group (B), the c-kit− cell-treated group (C) and the CT-treated group (D). (II): In the border zone, the vessel density of CT-treated group was significantly larger than the PBS-treated group (P < 0.05). (E) showing the semi-quantification analysis of vessel density. The representative morphologies of the PBS-treated group (F), c-kit− cell-treated group (G) and CT-treated group (H). Arrows indicate the vWF+ vessels; bar = 40 μm. N = 4–5 for each group. *P < 0.05, CT-treated group versusPBS-treated group.
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fig04: Transplantation of cardiac telocytes enhanced angiogenesis in myocardial infarction (MI). The density of vessels in MI hearts 14-week post-LAD ligation was determined by comparing the presence of vWF+ vessels in the infarct zone and border zone. (I) In the infarcted area, the vessel density of the CT-treated group was significantly larger than the PBS-treated group (P < 0.05). The vessel density of the c-kit− cell-treated group appeared larger than the PBS-treated group, but was not statistically significant (P > 0.05). (A) Showing the semi-quantification analysis of vessel density. The representative morphologies of the PBS-treated group (B), the c-kit− cell-treated group (C) and the CT-treated group (D). (II): In the border zone, the vessel density of CT-treated group was significantly larger than the PBS-treated group (P < 0.05). (E) showing the semi-quantification analysis of vessel density. The representative morphologies of the PBS-treated group (F), c-kit− cell-treated group (G) and CT-treated group (H). Arrows indicate the vWF+ vessels; bar = 40 μm. N = 4–5 for each group. *P < 0.05, CT-treated group versusPBS-treated group.

Mentions: The therapeutic effect of CT transplantation in promoting angiogenesis following MI was determined by comparing the presence of vWF+ vessels in the infarcted zone and the border zone. It was found that in the infarcted zone, the vessel density of CT-treated group was significantly larger than the PBS-treated group (P < 0.05). The vessel density of c-kit− cell-treated group was larger than the PBS-treated group, but the difference was not statistically significant (P > 0.05; Fig. 4IA-D). In addition, in border zone, the vessel density of CT-treated group was significantly larger than the PBS-treated group (P < 0.05). The vessel density of c-kit− cell-treated group was larger than the PBS-treated group, but the difference was not statistically significant (P > 0.05; Fig. 4IIE-H).


Intramyocardial transplantation of cardiac telocytes decreases myocardial infarction and improves post-infarcted cardiac function in rats.

Zhao B, Liao Z, Chen S, Yuan Z, Yilin C, Lee KK, Qi X, Shen X, Zheng X, Quinn T, Cai D - J. Cell. Mol. Med. (2014)

Transplantation of cardiac telocytes enhanced angiogenesis in myocardial infarction (MI). The density of vessels in MI hearts 14-week post-LAD ligation was determined by comparing the presence of vWF+ vessels in the infarct zone and border zone. (I) In the infarcted area, the vessel density of the CT-treated group was significantly larger than the PBS-treated group (P < 0.05). The vessel density of the c-kit− cell-treated group appeared larger than the PBS-treated group, but was not statistically significant (P > 0.05). (A) Showing the semi-quantification analysis of vessel density. The representative morphologies of the PBS-treated group (B), the c-kit− cell-treated group (C) and the CT-treated group (D). (II): In the border zone, the vessel density of CT-treated group was significantly larger than the PBS-treated group (P < 0.05). (E) showing the semi-quantification analysis of vessel density. The representative morphologies of the PBS-treated group (F), c-kit− cell-treated group (G) and CT-treated group (H). Arrows indicate the vWF+ vessels; bar = 40 μm. N = 4–5 for each group. *P < 0.05, CT-treated group versusPBS-treated group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4119384&req=5

fig04: Transplantation of cardiac telocytes enhanced angiogenesis in myocardial infarction (MI). The density of vessels in MI hearts 14-week post-LAD ligation was determined by comparing the presence of vWF+ vessels in the infarct zone and border zone. (I) In the infarcted area, the vessel density of the CT-treated group was significantly larger than the PBS-treated group (P < 0.05). The vessel density of the c-kit− cell-treated group appeared larger than the PBS-treated group, but was not statistically significant (P > 0.05). (A) Showing the semi-quantification analysis of vessel density. The representative morphologies of the PBS-treated group (B), the c-kit− cell-treated group (C) and the CT-treated group (D). (II): In the border zone, the vessel density of CT-treated group was significantly larger than the PBS-treated group (P < 0.05). (E) showing the semi-quantification analysis of vessel density. The representative morphologies of the PBS-treated group (F), c-kit− cell-treated group (G) and CT-treated group (H). Arrows indicate the vWF+ vessels; bar = 40 μm. N = 4–5 for each group. *P < 0.05, CT-treated group versusPBS-treated group.
Mentions: The therapeutic effect of CT transplantation in promoting angiogenesis following MI was determined by comparing the presence of vWF+ vessels in the infarcted zone and the border zone. It was found that in the infarcted zone, the vessel density of CT-treated group was significantly larger than the PBS-treated group (P < 0.05). The vessel density of c-kit− cell-treated group was larger than the PBS-treated group, but the difference was not statistically significant (P > 0.05; Fig. 4IA-D). In addition, in border zone, the vessel density of CT-treated group was significantly larger than the PBS-treated group (P < 0.05). The vessel density of c-kit− cell-treated group was larger than the PBS-treated group, but the difference was not statistically significant (P > 0.05; Fig. 4IIE-H).

Bottom Line: The cellular mechanism behind this beneficial effect on MI was partially attributed to increased cardiac angiogenesis, improved reconstruction of the CT network and decreased myocardial fibrosis.These combined effects decreased the infarct size, improved the reconstruction of the LV and enhanced myocardial function in MI.Our findings suggest that CTs could be considered as a potential cell source for therapeutic use to improve cardiac repair and function following MI, used either alone or in tandem with stem cells.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory for Regenerative Medicine, Ministry of Education, Ji Nan University, Guangzhou, China; Joint Laboratory for Regenerative Medicine, Chinese University of Hong Kong-Ji Nan University, Guangzhou, China; International Base of Collaboration for Science and Technology (JNU), The Ministry of Science and Technology & Guangdong Province, Guangzhou, China; Department of Developmental and Regenerative Biology, Ji Nan University, Guangzhou, China.

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Related in: MedlinePlus