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Effects of diclofenac and celecoxib on osteoclastogenesis during alveolar bone healing, in vivo.

Ghalayani P, Minaiyan M, Razavi SM, Hajisadeghi S, Naghsh N, Abuie MS - Dent Res J (Isfahan) (2014)

Bottom Line: Values of P < 0.05 were considered significant.On day 21, no significant differences were noted among the three studied groups.Both drugs affect RANKL/OPG gene expression and also osteoclastogenesis in alveolar socket during the experimental period of 21 days.

View Article: PubMed Central - PubMed

Affiliation: Torabinejad Dental Research Center and Department of Oral and Maxillofacial Medicine, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran.

ABSTRACT

Background: OSTEOCLASTOGENESIS IS COORDINATED BY THE INTERACTION OF MEMBERS OF THE TUMOR NECROSIS FACTOR (TNF) SUPERFAMILY: Receptor activator of nuclear factor-κB ligand (RANKL) and Osteoprotegerin (OPG). The aim of this study was to compare the effect of two different types of non-steroidal anti-inflammatory drugs (NSAIDs) on the RANKL/OPG balance during the healing of the alveolar process.

Materials and methods: This was an experimental study, carried on 45 male Wistar rats (200 ± 25 g, 8-10 weeks old). After extraction of the right maxillary first molar, 15 rats received 5 mg/kg/day of diclofenac and 15 rats received 15 mg/kg/day of celecoxib and 15 rats received normal saline. The animals were sacrificed 7, 14 and 21 days after tooth extraction. The number of osteoclasts, OPG and RANKL messenger ribonucleic acid expression were determined by tartrate-resistant acid phosphate (TRAP) staining and polymerase chain reaction (PCR) respectively. The data were analyzed by one-way ANOVA followed by Tukey's post-hoc test. Values of P < 0.05 were considered significant.

Results: On days 7, 14 and 21 the ratio of RANKL/OPG in the control group was higher than diclofenac and celecoxib groups. TRAP immunolabeling of the control group was more than diclofenac group on day 7 and was more than celecoxib group on day 14. On day 21, no significant differences were noted among the three studied groups.

Conclusion: Both drugs affect RANKL/OPG gene expression and also osteoclastogenesis in alveolar socket during the experimental period of 21 days.

No MeSH data available.


Related in: MedlinePlus

Comparison of variables between study groups. (a) Receptor activator of nuclear factor-κB ligand (RANKL) messenger ribonucleic acid (mRNA) expression in the bone marrow cells, (b) osteoprotegerin (OPG) mRNA expression in the bone marrow cells, (c) RANKL/OPG ratio, (d) tartrate-resistant acid phosphate. Significant difference was observed: * P< 0.05, ** P< 0.01. X axis: Time points. Y axis: Mean±standard error of the mean of variables P values derived from by Tukey's post-hoc test
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Figure 1: Comparison of variables between study groups. (a) Receptor activator of nuclear factor-κB ligand (RANKL) messenger ribonucleic acid (mRNA) expression in the bone marrow cells, (b) osteoprotegerin (OPG) mRNA expression in the bone marrow cells, (c) RANKL/OPG ratio, (d) tartrate-resistant acid phosphate. Significant difference was observed: * P< 0.05, ** P< 0.01. X axis: Time points. Y axis: Mean±standard error of the mean of variables P values derived from by Tukey's post-hoc test

Mentions: Results are expressed as relative expression in comparison to GAPDH. On the day 7, RANKL mRNA expression within extraction sockets was stimulated to the largest by the control group and this augmentation was suppressed by both NSAIDs. However, the difference between diclofenac and celecoxib groups was not statistically significant. On the day 14, only the control group showed higher RANKL mRNA expression than that of the day 7. Again, the control group had the highest RANKL mRNA expression and it was significantly inhibited in rat treated with diclofenac and celecoxib. This inhibition was slightly more noticeable in celecoxib. In the control group, RANKL gene expression level of the day 21 was higher than that of the day 7 and 14. RANKL gene expression of the control group was significantly higher than other groups [Figure 1a].


Effects of diclofenac and celecoxib on osteoclastogenesis during alveolar bone healing, in vivo.

Ghalayani P, Minaiyan M, Razavi SM, Hajisadeghi S, Naghsh N, Abuie MS - Dent Res J (Isfahan) (2014)

Comparison of variables between study groups. (a) Receptor activator of nuclear factor-κB ligand (RANKL) messenger ribonucleic acid (mRNA) expression in the bone marrow cells, (b) osteoprotegerin (OPG) mRNA expression in the bone marrow cells, (c) RANKL/OPG ratio, (d) tartrate-resistant acid phosphate. Significant difference was observed: * P< 0.05, ** P< 0.01. X axis: Time points. Y axis: Mean±standard error of the mean of variables P values derived from by Tukey's post-hoc test
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4119369&req=5

Figure 1: Comparison of variables between study groups. (a) Receptor activator of nuclear factor-κB ligand (RANKL) messenger ribonucleic acid (mRNA) expression in the bone marrow cells, (b) osteoprotegerin (OPG) mRNA expression in the bone marrow cells, (c) RANKL/OPG ratio, (d) tartrate-resistant acid phosphate. Significant difference was observed: * P< 0.05, ** P< 0.01. X axis: Time points. Y axis: Mean±standard error of the mean of variables P values derived from by Tukey's post-hoc test
Mentions: Results are expressed as relative expression in comparison to GAPDH. On the day 7, RANKL mRNA expression within extraction sockets was stimulated to the largest by the control group and this augmentation was suppressed by both NSAIDs. However, the difference between diclofenac and celecoxib groups was not statistically significant. On the day 14, only the control group showed higher RANKL mRNA expression than that of the day 7. Again, the control group had the highest RANKL mRNA expression and it was significantly inhibited in rat treated with diclofenac and celecoxib. This inhibition was slightly more noticeable in celecoxib. In the control group, RANKL gene expression level of the day 21 was higher than that of the day 7 and 14. RANKL gene expression of the control group was significantly higher than other groups [Figure 1a].

Bottom Line: Values of P < 0.05 were considered significant.On day 21, no significant differences were noted among the three studied groups.Both drugs affect RANKL/OPG gene expression and also osteoclastogenesis in alveolar socket during the experimental period of 21 days.

View Article: PubMed Central - PubMed

Affiliation: Torabinejad Dental Research Center and Department of Oral and Maxillofacial Medicine, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran.

ABSTRACT

Background: OSTEOCLASTOGENESIS IS COORDINATED BY THE INTERACTION OF MEMBERS OF THE TUMOR NECROSIS FACTOR (TNF) SUPERFAMILY: Receptor activator of nuclear factor-κB ligand (RANKL) and Osteoprotegerin (OPG). The aim of this study was to compare the effect of two different types of non-steroidal anti-inflammatory drugs (NSAIDs) on the RANKL/OPG balance during the healing of the alveolar process.

Materials and methods: This was an experimental study, carried on 45 male Wistar rats (200 ± 25 g, 8-10 weeks old). After extraction of the right maxillary first molar, 15 rats received 5 mg/kg/day of diclofenac and 15 rats received 15 mg/kg/day of celecoxib and 15 rats received normal saline. The animals were sacrificed 7, 14 and 21 days after tooth extraction. The number of osteoclasts, OPG and RANKL messenger ribonucleic acid expression were determined by tartrate-resistant acid phosphate (TRAP) staining and polymerase chain reaction (PCR) respectively. The data were analyzed by one-way ANOVA followed by Tukey's post-hoc test. Values of P < 0.05 were considered significant.

Results: On days 7, 14 and 21 the ratio of RANKL/OPG in the control group was higher than diclofenac and celecoxib groups. TRAP immunolabeling of the control group was more than diclofenac group on day 7 and was more than celecoxib group on day 14. On day 21, no significant differences were noted among the three studied groups.

Conclusion: Both drugs affect RANKL/OPG gene expression and also osteoclastogenesis in alveolar socket during the experimental period of 21 days.

No MeSH data available.


Related in: MedlinePlus