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IgE actions on CD4+ T cells, mast cells, and macrophages participate in the pathogenesis of experimental abdominal aortic aneurysms.

Wang J, Lindholt JS, Sukhova GK, Shi MA, Xia M, Chen H, Xiang M, He A, Wang Y, Xiong N, Libby P, Wang JA, Shi GP - EMBO Mol Med (2014)

Bottom Line: Immunoglobulin E (IgE) activates mast cells (MCs).This study demonstrates that CD4+ T cells express IgE receptor FcεR1, at much higher levels than do CD8+ T cells.IgE induces CD4+ T-cell production of IL6 and IFN-γ, but reduces their production of IL10.

View Article: PubMed Central - PubMed

Affiliation: Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA.

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Related in: MedlinePlus

IgE and FcεR1 expression in human AAAs lesionsA Immunostaining of human AAA lesion consecutive frozen sections to detect IgE and FcεR1 expression in CD68-positive macrophages and TUNEL reactivity. Mouse IgG and rabbit IgG were used as immunostaining negative controls for mouse anti-IgE and rabbit anti-FcεR1 antibodies.B Immunofluorescent double staining of CD4 and IgE or FcεR1 to detect IgE and FcεR1 expression in CD4+ T cells.C, D IgE and α-actin immunofluorescent double staining in smooth muscle cells (SMC)-rich and SMC-sparse areas.E FcεR1 and α-actin immunofluorescent double staining to detect FcεR1-positive SMCs.F CD31 and IgE or FcεR1 immunofluorescent double staining of human AAA lesions.Data information: Scale: 200 μm; insert scale: 60 μm. Arrows indicate IgE-positive or FcεR1-positive CD4+ T cells, SMCs or endothelial cells (ECs).
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fig08: IgE and FcεR1 expression in human AAAs lesionsA Immunostaining of human AAA lesion consecutive frozen sections to detect IgE and FcεR1 expression in CD68-positive macrophages and TUNEL reactivity. Mouse IgG and rabbit IgG were used as immunostaining negative controls for mouse anti-IgE and rabbit anti-FcεR1 antibodies.B Immunofluorescent double staining of CD4 and IgE or FcεR1 to detect IgE and FcεR1 expression in CD4+ T cells.C, D IgE and α-actin immunofluorescent double staining in smooth muscle cells (SMC)-rich and SMC-sparse areas.E FcεR1 and α-actin immunofluorescent double staining to detect FcεR1-positive SMCs.F CD31 and IgE or FcεR1 immunofluorescent double staining of human AAA lesions.Data information: Scale: 200 μm; insert scale: 60 μm. Arrows indicate IgE-positive or FcεR1-positive CD4+ T cells, SMCs or endothelial cells (ECs).

Mentions: We have previously shown that IgE activates human macrophages, SMCs, and ECs, and induces their apoptosis (Wang et al, 2011). The present study suggested a role of IgE stimulation of CD4+ T cells, MCs, and macrophages in experimental AAA formation. Inflammatory cells in human AAA lesions might also express IgE receptor FcεR1 and bind for IgE. Indeed, CD68-positive macrophages demonstrated expression of FcεR1 and IgE in consecutive frozen sections of human AAAs. Consistent with our prior study (Wang et al, 2011), IgE-positive and FcεR1-positive macrophages in human AAA lesions also underwent apoptosis as shown by TUNEL-positivity (Fig 8A). IgE binding to macrophages also triggers cytokine expression from these cells (Wang et al, 2011) as in CD4+ T cells. Immunofluorescent staining colocalized the expression of both IgE and FcεR1 on CD4+ T cells in human AAA lesions (Fig 8B).


IgE actions on CD4+ T cells, mast cells, and macrophages participate in the pathogenesis of experimental abdominal aortic aneurysms.

Wang J, Lindholt JS, Sukhova GK, Shi MA, Xia M, Chen H, Xiang M, He A, Wang Y, Xiong N, Libby P, Wang JA, Shi GP - EMBO Mol Med (2014)

IgE and FcεR1 expression in human AAAs lesionsA Immunostaining of human AAA lesion consecutive frozen sections to detect IgE and FcεR1 expression in CD68-positive macrophages and TUNEL reactivity. Mouse IgG and rabbit IgG were used as immunostaining negative controls for mouse anti-IgE and rabbit anti-FcεR1 antibodies.B Immunofluorescent double staining of CD4 and IgE or FcεR1 to detect IgE and FcεR1 expression in CD4+ T cells.C, D IgE and α-actin immunofluorescent double staining in smooth muscle cells (SMC)-rich and SMC-sparse areas.E FcεR1 and α-actin immunofluorescent double staining to detect FcεR1-positive SMCs.F CD31 and IgE or FcεR1 immunofluorescent double staining of human AAA lesions.Data information: Scale: 200 μm; insert scale: 60 μm. Arrows indicate IgE-positive or FcεR1-positive CD4+ T cells, SMCs or endothelial cells (ECs).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4119357&req=5

fig08: IgE and FcεR1 expression in human AAAs lesionsA Immunostaining of human AAA lesion consecutive frozen sections to detect IgE and FcεR1 expression in CD68-positive macrophages and TUNEL reactivity. Mouse IgG and rabbit IgG were used as immunostaining negative controls for mouse anti-IgE and rabbit anti-FcεR1 antibodies.B Immunofluorescent double staining of CD4 and IgE or FcεR1 to detect IgE and FcεR1 expression in CD4+ T cells.C, D IgE and α-actin immunofluorescent double staining in smooth muscle cells (SMC)-rich and SMC-sparse areas.E FcεR1 and α-actin immunofluorescent double staining to detect FcεR1-positive SMCs.F CD31 and IgE or FcεR1 immunofluorescent double staining of human AAA lesions.Data information: Scale: 200 μm; insert scale: 60 μm. Arrows indicate IgE-positive or FcεR1-positive CD4+ T cells, SMCs or endothelial cells (ECs).
Mentions: We have previously shown that IgE activates human macrophages, SMCs, and ECs, and induces their apoptosis (Wang et al, 2011). The present study suggested a role of IgE stimulation of CD4+ T cells, MCs, and macrophages in experimental AAA formation. Inflammatory cells in human AAA lesions might also express IgE receptor FcεR1 and bind for IgE. Indeed, CD68-positive macrophages demonstrated expression of FcεR1 and IgE in consecutive frozen sections of human AAAs. Consistent with our prior study (Wang et al, 2011), IgE-positive and FcεR1-positive macrophages in human AAA lesions also underwent apoptosis as shown by TUNEL-positivity (Fig 8A). IgE binding to macrophages also triggers cytokine expression from these cells (Wang et al, 2011) as in CD4+ T cells. Immunofluorescent staining colocalized the expression of both IgE and FcεR1 on CD4+ T cells in human AAA lesions (Fig 8B).

Bottom Line: Immunoglobulin E (IgE) activates mast cells (MCs).This study demonstrates that CD4+ T cells express IgE receptor FcεR1, at much higher levels than do CD8+ T cells.IgE induces CD4+ T-cell production of IL6 and IFN-γ, but reduces their production of IL10.

View Article: PubMed Central - PubMed

Affiliation: Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA.

Show MeSH
Related in: MedlinePlus