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ANO1 as a marker of oral squamous cell carcinoma and silencing ANO1 suppresses migration of human SCC-25 cells.

Li Y, Zhang J, Hong S - Med Oral Patol Oral Cir Bucal (2014)

Bottom Line: Our study shows that abnormal expression of ANO1 correlated with the occurrence and metastasis of OSCC in clinical specimens and that silencing ANO1 greatly reduced migration ability of scc-25 cells.Calcium activated chloride channel activity of ANO1 promoted the cell migration.Thus, ANO1 could represent a new diagnostic biomarker and a potentially important therapeutic target of OSCC.

View Article: PubMed Central - PubMed

Affiliation: Department of Otolaryngology, The First Affiliated Hospital of Chongqing Medical University, No.400016, Chongqing, China, llxxyydd2006@sina.com.

ABSTRACT

Objectives: The purpose of this study is to confirm that ANO1 correlates with occurrence and metastasis of OSCC.

Study design: Immunohistochemistry was used to detect the expression of ANO1 in 160 specimens of OSCC and normal tissues. Lentiviral silencing ANO1 was used in SCC-25 cell line to study the cell migration and cell detachment.

Results: Immunohistochemical staining revealed that ANO1 was expressed in a large majority (132 out of 160, 82.5%) of OSCC specimens and that the rate of ANO1 expression in OSCC was significantly higher than that of normal tissue (P<0.05); The rate of ANO1 expression was higher in metastatic tumors than in non-metastatic tumors, and the difference was significant (P<0.05). The results of cell migration assay showed that the percentage of cells through the membrane was 26.61 ±0.81 in assay group, and 54.26 ±3.74 in control group, respectively (t=-16.22,P<0.0001). The results of cell detachment assay showed that the percentage of cells detachment was 37.42 ±0.90 in assay group, and 87.38 ±1.59 in control group, respectively (t=-62.34, P<0.0001). The results of wound healing assay showed the assay group had a reduced migration rate compared with the control group in 32 h (F=1038.78, P<0.0001). Wound closure was no significantly different between the assay and control cells when DIDS was used in wound healing assay (F=4.61,P>0.05).

Conclusions: Our study shows that abnormal expression of ANO1 correlated with the occurrence and metastasis of OSCC in clinical specimens and that silencing ANO1 greatly reduced migration ability of scc-25 cells. Calcium activated chloride channel activity of ANO1 promoted the cell migration. Thus, ANO1 could represent a new diagnostic biomarker and a potentially important therapeutic target of OSCC.

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DIDS decreases scc-25 cell migration in control cells. A,B: To investigate whether calcium activated chloride channel activity of ANO1 promote the cell migration, the inhibitor (DIDS) was used in vitro wound healing assay. The results showed that wound closure were no signifcantly different between the assay and control cells (F=4.61, P>0.05)
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Figure 3: DIDS decreases scc-25 cell migration in control cells. A,B: To investigate whether calcium activated chloride channel activity of ANO1 promote the cell migration, the inhibitor (DIDS) was used in vitro wound healing assay. The results showed that wound closure were no signifcantly different between the assay and control cells (F=4.61, P>0.05)

Mentions: To investigate whether calcium activated chloride channel activity of ANO1 promotes the cell migration, DIDS, an inhibitor of anion exchange channel, was used in in vitro wound healing assay. The results showed that DIDS delayed wound closure in the control cells, since the expression of ANO1 was not knockdown. The lack of an effect on assay cells is expected because of si-lencing ANO1. So wound closure was no significantly different between the assay and control cells (F=4.61, P>0.05) (Fig. 3), which showed that ANO1 channel activity participated in the scc-25 cell migration.


ANO1 as a marker of oral squamous cell carcinoma and silencing ANO1 suppresses migration of human SCC-25 cells.

Li Y, Zhang J, Hong S - Med Oral Patol Oral Cir Bucal (2014)

DIDS decreases scc-25 cell migration in control cells. A,B: To investigate whether calcium activated chloride channel activity of ANO1 promote the cell migration, the inhibitor (DIDS) was used in vitro wound healing assay. The results showed that wound closure were no signifcantly different between the assay and control cells (F=4.61, P>0.05)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4119304&req=5

Figure 3: DIDS decreases scc-25 cell migration in control cells. A,B: To investigate whether calcium activated chloride channel activity of ANO1 promote the cell migration, the inhibitor (DIDS) was used in vitro wound healing assay. The results showed that wound closure were no signifcantly different between the assay and control cells (F=4.61, P>0.05)
Mentions: To investigate whether calcium activated chloride channel activity of ANO1 promotes the cell migration, DIDS, an inhibitor of anion exchange channel, was used in in vitro wound healing assay. The results showed that DIDS delayed wound closure in the control cells, since the expression of ANO1 was not knockdown. The lack of an effect on assay cells is expected because of si-lencing ANO1. So wound closure was no significantly different between the assay and control cells (F=4.61, P>0.05) (Fig. 3), which showed that ANO1 channel activity participated in the scc-25 cell migration.

Bottom Line: Our study shows that abnormal expression of ANO1 correlated with the occurrence and metastasis of OSCC in clinical specimens and that silencing ANO1 greatly reduced migration ability of scc-25 cells.Calcium activated chloride channel activity of ANO1 promoted the cell migration.Thus, ANO1 could represent a new diagnostic biomarker and a potentially important therapeutic target of OSCC.

View Article: PubMed Central - PubMed

Affiliation: Department of Otolaryngology, The First Affiliated Hospital of Chongqing Medical University, No.400016, Chongqing, China, llxxyydd2006@sina.com.

ABSTRACT

Objectives: The purpose of this study is to confirm that ANO1 correlates with occurrence and metastasis of OSCC.

Study design: Immunohistochemistry was used to detect the expression of ANO1 in 160 specimens of OSCC and normal tissues. Lentiviral silencing ANO1 was used in SCC-25 cell line to study the cell migration and cell detachment.

Results: Immunohistochemical staining revealed that ANO1 was expressed in a large majority (132 out of 160, 82.5%) of OSCC specimens and that the rate of ANO1 expression in OSCC was significantly higher than that of normal tissue (P<0.05); The rate of ANO1 expression was higher in metastatic tumors than in non-metastatic tumors, and the difference was significant (P<0.05). The results of cell migration assay showed that the percentage of cells through the membrane was 26.61 ±0.81 in assay group, and 54.26 ±3.74 in control group, respectively (t=-16.22,P<0.0001). The results of cell detachment assay showed that the percentage of cells detachment was 37.42 ±0.90 in assay group, and 87.38 ±1.59 in control group, respectively (t=-62.34, P<0.0001). The results of wound healing assay showed the assay group had a reduced migration rate compared with the control group in 32 h (F=1038.78, P<0.0001). Wound closure was no significantly different between the assay and control cells when DIDS was used in wound healing assay (F=4.61,P>0.05).

Conclusions: Our study shows that abnormal expression of ANO1 correlated with the occurrence and metastasis of OSCC in clinical specimens and that silencing ANO1 greatly reduced migration ability of scc-25 cells. Calcium activated chloride channel activity of ANO1 promoted the cell migration. Thus, ANO1 could represent a new diagnostic biomarker and a potentially important therapeutic target of OSCC.

Show MeSH
Related in: MedlinePlus