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Topical application of phosphatidyl-inositol-3,5-bisphosphate for acute lung injury in neonatal swine.

Preuss S, Omam FD, Scheiermann J, Stadelmann S, Winoto-Morbach S, von Bismarck P, Adam-Klages S, Knerlich-Lukoschus F, Lex D, Wesch D, Held-Feindt J, Uhlig S, Schütze S, Krause MF - J. Cell. Mol. Med. (2012)

Bottom Line: Only in the latter two groups we observed significantly improved oxygenation and ventilation, dynamic compliance and pulmonary oedema.S+Imi caused systemic aSMase suppression and ceramide reduction, whereas the S+PIP2 effect remained compartmentalized in the airways because of the molecule's bulky structure.Finally we observed reduced alveolar epithelial apoptosis, which was most apparent in S+PIP2 lungs.

View Article: PubMed Central - PubMed

Affiliation: Universitätsklinikum Schleswig-Holstein, Campus Kiel, Department of Pediatrics, Kiel, Germany.

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aSMase activity (A) and ceramide concentrations in pulmonary tissues (B) after 72 hrs of mechanical ventilation; aSMase activity in the BALF (C) from the initial lavage and after 72 hrs, S+Imi and S+PIP2 groups only, aSMase activity in serum over time (D), S+Imi and S+PIP2 groups only. Data represent the means ± SEM, univariate anova followed by Dunnett's post-tests in A+B, unpaired t-tests in C+D.
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fig07: aSMase activity (A) and ceramide concentrations in pulmonary tissues (B) after 72 hrs of mechanical ventilation; aSMase activity in the BALF (C) from the initial lavage and after 72 hrs, S+Imi and S+PIP2 groups only, aSMase activity in serum over time (D), S+Imi and S+PIP2 groups only. Data represent the means ± SEM, univariate anova followed by Dunnett's post-tests in A+B, unpaired t-tests in C+D.

Mentions: Compared to the C group, S+Imi reduced aSMase activity in pulmonary tissues by 41.9% (Fig. 7A). There was a 33.2% reduction in the levels of ceramide (Fig. 7B). Because the reduction in aSMase activity in S+PIP2 treated lungs was similar to the S-treated lungs, and no additional effect on aSMase inhibition could be detected, we also compared aSMase activities in the alveolar (Fig. 7C) and blood (Fig. 7D) compartments. The results show that 1. the local alveolar inhibition was even more pronounced (but not significantly different) after treatment with S+PIP2 compared to S+Imi, and 2. only S+Imi, but not S+PIP2, exerted a systemic effect by balancing the aSMase activity levels in serum over time.


Topical application of phosphatidyl-inositol-3,5-bisphosphate for acute lung injury in neonatal swine.

Preuss S, Omam FD, Scheiermann J, Stadelmann S, Winoto-Morbach S, von Bismarck P, Adam-Klages S, Knerlich-Lukoschus F, Lex D, Wesch D, Held-Feindt J, Uhlig S, Schütze S, Krause MF - J. Cell. Mol. Med. (2012)

aSMase activity (A) and ceramide concentrations in pulmonary tissues (B) after 72 hrs of mechanical ventilation; aSMase activity in the BALF (C) from the initial lavage and after 72 hrs, S+Imi and S+PIP2 groups only, aSMase activity in serum over time (D), S+Imi and S+PIP2 groups only. Data represent the means ± SEM, univariate anova followed by Dunnett's post-tests in A+B, unpaired t-tests in C+D.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4118249&req=5

fig07: aSMase activity (A) and ceramide concentrations in pulmonary tissues (B) after 72 hrs of mechanical ventilation; aSMase activity in the BALF (C) from the initial lavage and after 72 hrs, S+Imi and S+PIP2 groups only, aSMase activity in serum over time (D), S+Imi and S+PIP2 groups only. Data represent the means ± SEM, univariate anova followed by Dunnett's post-tests in A+B, unpaired t-tests in C+D.
Mentions: Compared to the C group, S+Imi reduced aSMase activity in pulmonary tissues by 41.9% (Fig. 7A). There was a 33.2% reduction in the levels of ceramide (Fig. 7B). Because the reduction in aSMase activity in S+PIP2 treated lungs was similar to the S-treated lungs, and no additional effect on aSMase inhibition could be detected, we also compared aSMase activities in the alveolar (Fig. 7C) and blood (Fig. 7D) compartments. The results show that 1. the local alveolar inhibition was even more pronounced (but not significantly different) after treatment with S+PIP2 compared to S+Imi, and 2. only S+Imi, but not S+PIP2, exerted a systemic effect by balancing the aSMase activity levels in serum over time.

Bottom Line: Only in the latter two groups we observed significantly improved oxygenation and ventilation, dynamic compliance and pulmonary oedema.S+Imi caused systemic aSMase suppression and ceramide reduction, whereas the S+PIP2 effect remained compartmentalized in the airways because of the molecule's bulky structure.Finally we observed reduced alveolar epithelial apoptosis, which was most apparent in S+PIP2 lungs.

View Article: PubMed Central - PubMed

Affiliation: Universitätsklinikum Schleswig-Holstein, Campus Kiel, Department of Pediatrics, Kiel, Germany.

Show MeSH
Related in: MedlinePlus