Topical application of phosphatidyl-inositol-3,5-bisphosphate for acute lung injury in neonatal swine.
Bottom Line: Only in the latter two groups we observed significantly improved oxygenation and ventilation, dynamic compliance and pulmonary oedema.S+Imi caused systemic aSMase suppression and ceramide reduction, whereas the S+PIP2 effect remained compartmentalized in the airways because of the molecule's bulky structure.Finally we observed reduced alveolar epithelial apoptosis, which was most apparent in S+PIP2 lungs.
Affiliation: Universitätsklinikum Schleswig-Holstein, Campus Kiel, Department of Pediatrics, Kiel, Germany.Show MeSH
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Mentions: Compared to the C group, S+Imi reduced aSMase activity in pulmonary tissues by 41.9% (Fig. 7A). There was a 33.2% reduction in the levels of ceramide (Fig. 7B). Because the reduction in aSMase activity in S+PIP2 treated lungs was similar to the S-treated lungs, and no additional effect on aSMase inhibition could be detected, we also compared aSMase activities in the alveolar (Fig. 7C) and blood (Fig. 7D) compartments. The results show that 1. the local alveolar inhibition was even more pronounced (but not significantly different) after treatment with S+PIP2 compared to S+Imi, and 2. only S+Imi, but not S+PIP2, exerted a systemic effect by balancing the aSMase activity levels in serum over time.
Affiliation: Universitätsklinikum Schleswig-Holstein, Campus Kiel, Department of Pediatrics, Kiel, Germany.