Monocytic microparticles promote atherogenesis by modulating inflammatory cells in mice.
Bottom Line: In vitro, incubation of mono-MP with murine macrophages and endothelial cells resulted in the uptake of calcein-labelled mono-MP.Murine macrophages pre-treated with mono-MP showed significantly enhanced expression of CCR2, migration to MCP-1 and increased release of pro-inflammatory interleukin-6.Co-incubation of mono-MP with endothelial cells resulted in significantly increased expression of ICAM-1, as assessed by RT-PCR and ELISA.
Affiliation: Medizinische Klinik und Poliklinik II, Universitätsklinikum Bonn, Bonn, Germany.Show MeSH
Related in: MedlinePlus
Mentions: Enhanced levels of reactive oxygen species occur during atherogenesis and foster progression of vascular inflammation. Therefore, we have assessed whether mono-MP influence oxidative stress in murine macrophages. A total of 1 × 106 murine bone marrow-derived macrophages per 6 cm dish were pre-treated with 1 × 106 mono-MP or vehicle, on day 7 of differentiation, for 4 hrs at 37°C and intracellular ROS generation was determined by means of C2′,7′-dichlorodihydrofluorescin-diacetate. Mono-MP application markedly increased oxidative stress in murine macrophages compared with control (mono-MP: 120.2 ± 4.9% versus heat-inactivated mono-MP: 104.6 ± 3.9%, n = 4, P < 0.05) (Fig. 7).
Affiliation: Medizinische Klinik und Poliklinik II, Universitätsklinikum Bonn, Bonn, Germany.