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Monocytic microparticles promote atherogenesis by modulating inflammatory cells in mice.

Hoyer FF, Giesen MK, Nunes França C, Lütjohann D, Nickenig G, Werner N - J. Cell. Mol. Med. (2012)

Bottom Line: In vitro, incubation of mono-MP with murine macrophages and endothelial cells resulted in the uptake of calcein-labelled mono-MP.Murine macrophages pre-treated with mono-MP showed significantly enhanced expression of CCR2, migration to MCP-1 and increased release of pro-inflammatory interleukin-6.Co-incubation of mono-MP with endothelial cells resulted in significantly increased expression of ICAM-1, as assessed by RT-PCR and ELISA.

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Affiliation: Medizinische Klinik und Poliklinik II, Universitätsklinikum Bonn, Bonn, Germany.

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Generation of reactive oxygen species in murine macrophages.To assess the influence of mono-MP on the generation of intracellular oxidative stress in macrophages, C2′,7′-dichlorodihydrofluorescin-diacetate experiments were performed. Murine bone marrow-derived macrophages were stimulated with mono-MP or vehicle for 4 hrs on day 7 of differentiation. Cells pre-treated with mono-MP showed significantly increased intracellular ROS generation compared with control (mono-MP: 120.2 ± 4.9% versus heat inactivated mono-MP: 104.6 ± 3.9%, n = 4, * denotes P < 0.05).
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fig07: Generation of reactive oxygen species in murine macrophages.To assess the influence of mono-MP on the generation of intracellular oxidative stress in macrophages, C2′,7′-dichlorodihydrofluorescin-diacetate experiments were performed. Murine bone marrow-derived macrophages were stimulated with mono-MP or vehicle for 4 hrs on day 7 of differentiation. Cells pre-treated with mono-MP showed significantly increased intracellular ROS generation compared with control (mono-MP: 120.2 ± 4.9% versus heat inactivated mono-MP: 104.6 ± 3.9%, n = 4, * denotes P < 0.05).

Mentions: Enhanced levels of reactive oxygen species occur during atherogenesis and foster progression of vascular inflammation. Therefore, we have assessed whether mono-MP influence oxidative stress in murine macrophages. A total of 1 × 106 murine bone marrow-derived macrophages per 6 cm dish were pre-treated with 1 × 106 mono-MP or vehicle, on day 7 of differentiation, for 4 hrs at 37°C and intracellular ROS generation was determined by means of C2′,7′-dichlorodihydrofluorescin-diacetate. Mono-MP application markedly increased oxidative stress in murine macrophages compared with control (mono-MP: 120.2 ± 4.9% versus heat-inactivated mono-MP: 104.6 ± 3.9%, n = 4, P < 0.05) (Fig. 7).


Monocytic microparticles promote atherogenesis by modulating inflammatory cells in mice.

Hoyer FF, Giesen MK, Nunes França C, Lütjohann D, Nickenig G, Werner N - J. Cell. Mol. Med. (2012)

Generation of reactive oxygen species in murine macrophages.To assess the influence of mono-MP on the generation of intracellular oxidative stress in macrophages, C2′,7′-dichlorodihydrofluorescin-diacetate experiments were performed. Murine bone marrow-derived macrophages were stimulated with mono-MP or vehicle for 4 hrs on day 7 of differentiation. Cells pre-treated with mono-MP showed significantly increased intracellular ROS generation compared with control (mono-MP: 120.2 ± 4.9% versus heat inactivated mono-MP: 104.6 ± 3.9%, n = 4, * denotes P < 0.05).
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Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4118246&req=5

fig07: Generation of reactive oxygen species in murine macrophages.To assess the influence of mono-MP on the generation of intracellular oxidative stress in macrophages, C2′,7′-dichlorodihydrofluorescin-diacetate experiments were performed. Murine bone marrow-derived macrophages were stimulated with mono-MP or vehicle for 4 hrs on day 7 of differentiation. Cells pre-treated with mono-MP showed significantly increased intracellular ROS generation compared with control (mono-MP: 120.2 ± 4.9% versus heat inactivated mono-MP: 104.6 ± 3.9%, n = 4, * denotes P < 0.05).
Mentions: Enhanced levels of reactive oxygen species occur during atherogenesis and foster progression of vascular inflammation. Therefore, we have assessed whether mono-MP influence oxidative stress in murine macrophages. A total of 1 × 106 murine bone marrow-derived macrophages per 6 cm dish were pre-treated with 1 × 106 mono-MP or vehicle, on day 7 of differentiation, for 4 hrs at 37°C and intracellular ROS generation was determined by means of C2′,7′-dichlorodihydrofluorescin-diacetate. Mono-MP application markedly increased oxidative stress in murine macrophages compared with control (mono-MP: 120.2 ± 4.9% versus heat-inactivated mono-MP: 104.6 ± 3.9%, n = 4, P < 0.05) (Fig. 7).

Bottom Line: In vitro, incubation of mono-MP with murine macrophages and endothelial cells resulted in the uptake of calcein-labelled mono-MP.Murine macrophages pre-treated with mono-MP showed significantly enhanced expression of CCR2, migration to MCP-1 and increased release of pro-inflammatory interleukin-6.Co-incubation of mono-MP with endothelial cells resulted in significantly increased expression of ICAM-1, as assessed by RT-PCR and ELISA.

View Article: PubMed Central - PubMed

Affiliation: Medizinische Klinik und Poliklinik II, Universitätsklinikum Bonn, Bonn, Germany.

Show MeSH
Related in: MedlinePlus