Monocytic microparticles promote atherogenesis by modulating inflammatory cells in mice.
Bottom Line: In vitro, incubation of mono-MP with murine macrophages and endothelial cells resulted in the uptake of calcein-labelled mono-MP.Murine macrophages pre-treated with mono-MP showed significantly enhanced expression of CCR2, migration to MCP-1 and increased release of pro-inflammatory interleukin-6.Co-incubation of mono-MP with endothelial cells resulted in significantly increased expression of ICAM-1, as assessed by RT-PCR and ELISA.
Affiliation: Medizinische Klinik und Poliklinik II, Universitätsklinikum Bonn, Bonn, Germany.Show MeSH
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Mentions: To assess general pro-inflammatory responses provoked by mono-MP, IL-6 liberation of murine macrophages was determined after mono-MP application. A total of 1 × 105 murine macrophages per 24-well were stimulated for 24 hrs with 1 × 105 mono-MP or vehicle on day 6 of differentiation. Mono-MP activation significantly increased the release of IL-6 compared with control, as assessed by ELISA (mono-MP: 238.2 ± 17.64% versus vehicle: 100.0 ± 3.7%, n = 3, P < 0.05) (Fig. 6).
Affiliation: Medizinische Klinik und Poliklinik II, Universitätsklinikum Bonn, Bonn, Germany.