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Transendocardial cell injection is not superior to intracoronary infusion in a porcine model of ischaemic cardiomyopathy: a study on delivery efficiency.

van der Spoel TI, Vrijsen KR, Koudstaal S, Sluijter JP, Nijsen JF, de Jong HW, Hoefer IE, Cramer MJ, Doevendans PA, van Belle E, Chamuleau SA - J. Cell. Mol. Med. (2012)

Bottom Line: IC showed less variability in efficiency compared with TE and surgical injection.Troponin rise and IC flow did not differ between the percutaneous groups.In addition, no differences in safety profile were observed.

View Article: PubMed Central - PubMed

Affiliation: Division Heart and Lungs, Department of Cardiology, University Medical Center Utrecht, Utrecht, The Netherlands. tspoel@umcutrecht.nl

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Segmental analysis of the LV after percutaneous delivery. To visualize cell retention in the LV, the heart was cut into five slices from apex to base (histological images). Static anterior images were obtained from all slices within 4 hrs after cell delivery. Note that after TE delivery, cells were mainly retained in the midlateral wall whereas IC infusion showed a more scattered distribution of MSC in the posterolateral wall. Representative histological and nuclear images were derived from the same animals for both groups. Black arrow: left anterior descending artery; IC: intracoronary; L: lateral wall; TE: transendocardial; White dotted lines: endocardial and epicardial border; S: septal wall; Black dotted line: area of interest.
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fig03: Segmental analysis of the LV after percutaneous delivery. To visualize cell retention in the LV, the heart was cut into five slices from apex to base (histological images). Static anterior images were obtained from all slices within 4 hrs after cell delivery. Note that after TE delivery, cells were mainly retained in the midlateral wall whereas IC infusion showed a more scattered distribution of MSC in the posterolateral wall. Representative histological and nuclear images were derived from the same animals for both groups. Black arrow: left anterior descending artery; IC: intracoronary; L: lateral wall; TE: transendocardial; White dotted lines: endocardial and epicardial border; S: septal wall; Black dotted line: area of interest.

Mentions: Whole-body γ-scan revealed a trend towards higher retention of MSC after surgical delivery (16 ± 4%) compared with IC (11 ± 1%) and TE (11 ± 3%), but this difference was not statistically significant (P = 0.52). Variation in delivery efficiency was less in the IC group (Fig. 2). Qualitative analysis after TE delivery showed a higher local retention of cells at the mid-papillary level in the targeted area compared with widespread distribution of cells in the infarcted area after IC infusion (Fig. 3).


Transendocardial cell injection is not superior to intracoronary infusion in a porcine model of ischaemic cardiomyopathy: a study on delivery efficiency.

van der Spoel TI, Vrijsen KR, Koudstaal S, Sluijter JP, Nijsen JF, de Jong HW, Hoefer IE, Cramer MJ, Doevendans PA, van Belle E, Chamuleau SA - J. Cell. Mol. Med. (2012)

Segmental analysis of the LV after percutaneous delivery. To visualize cell retention in the LV, the heart was cut into five slices from apex to base (histological images). Static anterior images were obtained from all slices within 4 hrs after cell delivery. Note that after TE delivery, cells were mainly retained in the midlateral wall whereas IC infusion showed a more scattered distribution of MSC in the posterolateral wall. Representative histological and nuclear images were derived from the same animals for both groups. Black arrow: left anterior descending artery; IC: intracoronary; L: lateral wall; TE: transendocardial; White dotted lines: endocardial and epicardial border; S: septal wall; Black dotted line: area of interest.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4118245&req=5

fig03: Segmental analysis of the LV after percutaneous delivery. To visualize cell retention in the LV, the heart was cut into five slices from apex to base (histological images). Static anterior images were obtained from all slices within 4 hrs after cell delivery. Note that after TE delivery, cells were mainly retained in the midlateral wall whereas IC infusion showed a more scattered distribution of MSC in the posterolateral wall. Representative histological and nuclear images were derived from the same animals for both groups. Black arrow: left anterior descending artery; IC: intracoronary; L: lateral wall; TE: transendocardial; White dotted lines: endocardial and epicardial border; S: septal wall; Black dotted line: area of interest.
Mentions: Whole-body γ-scan revealed a trend towards higher retention of MSC after surgical delivery (16 ± 4%) compared with IC (11 ± 1%) and TE (11 ± 3%), but this difference was not statistically significant (P = 0.52). Variation in delivery efficiency was less in the IC group (Fig. 2). Qualitative analysis after TE delivery showed a higher local retention of cells at the mid-papillary level in the targeted area compared with widespread distribution of cells in the infarcted area after IC infusion (Fig. 3).

Bottom Line: IC showed less variability in efficiency compared with TE and surgical injection.Troponin rise and IC flow did not differ between the percutaneous groups.In addition, no differences in safety profile were observed.

View Article: PubMed Central - PubMed

Affiliation: Division Heart and Lungs, Department of Cardiology, University Medical Center Utrecht, Utrecht, The Netherlands. tspoel@umcutrecht.nl

Show MeSH
Related in: MedlinePlus