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Intrapleural delivery of MSCs attenuates acute lung injury by paracrine/endocrine mechanism.

Qin ZH, Xu JF, Qu JM, Zhang J, Sai Y, Chen CM, Wu L, Yu L - J. Cell. Mol. Med. (2012)

Bottom Line: MSCs intrapleural injection significantly improved LPS-induced lung histopathology compared with PBS-treated group at day 3.There was also a significant decrease in total cell counts and protein concentration in BALF at day 7 in the MSCs -treated rats compared to PBS control group.In conclusion, treatment with intrapleural MSCs administration markedly attenuates the severity of endotoxin-induced ALI.

View Article: PubMed Central - PubMed

Affiliation: Department of Pulmonary Medicine, Huadong Hospital, Shanghai Medical College, Fudan University, Shanghai, China.

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Levels of pro- and anti-inflammatory cytokines. LPS-induced increase of TNF-α in BALF was significantly reduced in the MSCs treatment group at day 3, whereas IL-10 in lung tissue was significantly increased at day 1 at both protein and at mRNA levels with MSCs therapy. (A, C, E) Levels of TNF-α in BALF, lung tissue and in mRNA respectively. (B, D, F) Levels of IL-10 in BALF, lung tissue and in mRNA respectively. Data are expressed as mean ± SD, n = 6 per group per time-point. *P < 0.05 versus the PBS treatment group at the same time-point, #P < 0.05 versus normal group, ##P < 0.01 versus normal group. Data were assessed using one-way analysis of variance with SNK-q post hoc tests.
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fig08: Levels of pro- and anti-inflammatory cytokines. LPS-induced increase of TNF-α in BALF was significantly reduced in the MSCs treatment group at day 3, whereas IL-10 in lung tissue was significantly increased at day 1 at both protein and at mRNA levels with MSCs therapy. (A, C, E) Levels of TNF-α in BALF, lung tissue and in mRNA respectively. (B, D, F) Levels of IL-10 in BALF, lung tissue and in mRNA respectively. Data are expressed as mean ± SD, n = 6 per group per time-point. *P < 0.05 versus the PBS treatment group at the same time-point, #P < 0.05 versus normal group, ##P < 0.01 versus normal group. Data were assessed using one-way analysis of variance with SNK-q post hoc tests.

Mentions: We detected the levels of pro-inflammatory cytokine TNF-α and anti-inflammatory cytokine IL-10 in lung tissue and BALF by ELISA. The mRNA levels of these cytokines in lung tissue were also examined by real-time RT-PCR. At day 3, TNF-α in BALF was significantly reduced in the MSCs treatment group compared with that in PBS treatment group (P < 0.05) (Fig. 8). Similar results of TNF-α protein were observed in lung tissue. TNF-αmRNA expression in lung tissue increased 3 days after LPS exposure, although no significant difference between the MSCs- and PBS- treatment groups was observed at all time-points. At day 1, the level of IL-10 in lung tissue was significantly higher in the MSCs treatment group than that in the PBS treatment group (P < 0.05) (Fig. 8), same with the results of IL-10 mRNA expression (P < 0.05) (Fig. 8). LPS induced a significant increase of IL-10 in BALF at day 1, then gradually decreased to the normal levels at day 7. The levels of IL-10 protein in BALF showed no difference between the MSCs group and the PBS group.


Intrapleural delivery of MSCs attenuates acute lung injury by paracrine/endocrine mechanism.

Qin ZH, Xu JF, Qu JM, Zhang J, Sai Y, Chen CM, Wu L, Yu L - J. Cell. Mol. Med. (2012)

Levels of pro- and anti-inflammatory cytokines. LPS-induced increase of TNF-α in BALF was significantly reduced in the MSCs treatment group at day 3, whereas IL-10 in lung tissue was significantly increased at day 1 at both protein and at mRNA levels with MSCs therapy. (A, C, E) Levels of TNF-α in BALF, lung tissue and in mRNA respectively. (B, D, F) Levels of IL-10 in BALF, lung tissue and in mRNA respectively. Data are expressed as mean ± SD, n = 6 per group per time-point. *P < 0.05 versus the PBS treatment group at the same time-point, #P < 0.05 versus normal group, ##P < 0.01 versus normal group. Data were assessed using one-way analysis of variance with SNK-q post hoc tests.
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Related In: Results  -  Collection

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fig08: Levels of pro- and anti-inflammatory cytokines. LPS-induced increase of TNF-α in BALF was significantly reduced in the MSCs treatment group at day 3, whereas IL-10 in lung tissue was significantly increased at day 1 at both protein and at mRNA levels with MSCs therapy. (A, C, E) Levels of TNF-α in BALF, lung tissue and in mRNA respectively. (B, D, F) Levels of IL-10 in BALF, lung tissue and in mRNA respectively. Data are expressed as mean ± SD, n = 6 per group per time-point. *P < 0.05 versus the PBS treatment group at the same time-point, #P < 0.05 versus normal group, ##P < 0.01 versus normal group. Data were assessed using one-way analysis of variance with SNK-q post hoc tests.
Mentions: We detected the levels of pro-inflammatory cytokine TNF-α and anti-inflammatory cytokine IL-10 in lung tissue and BALF by ELISA. The mRNA levels of these cytokines in lung tissue were also examined by real-time RT-PCR. At day 3, TNF-α in BALF was significantly reduced in the MSCs treatment group compared with that in PBS treatment group (P < 0.05) (Fig. 8). Similar results of TNF-α protein were observed in lung tissue. TNF-αmRNA expression in lung tissue increased 3 days after LPS exposure, although no significant difference between the MSCs- and PBS- treatment groups was observed at all time-points. At day 1, the level of IL-10 in lung tissue was significantly higher in the MSCs treatment group than that in the PBS treatment group (P < 0.05) (Fig. 8), same with the results of IL-10 mRNA expression (P < 0.05) (Fig. 8). LPS induced a significant increase of IL-10 in BALF at day 1, then gradually decreased to the normal levels at day 7. The levels of IL-10 protein in BALF showed no difference between the MSCs group and the PBS group.

Bottom Line: MSCs intrapleural injection significantly improved LPS-induced lung histopathology compared with PBS-treated group at day 3.There was also a significant decrease in total cell counts and protein concentration in BALF at day 7 in the MSCs -treated rats compared to PBS control group.In conclusion, treatment with intrapleural MSCs administration markedly attenuates the severity of endotoxin-induced ALI.

View Article: PubMed Central - PubMed

Affiliation: Department of Pulmonary Medicine, Huadong Hospital, Shanghai Medical College, Fudan University, Shanghai, China.

Show MeSH
Related in: MedlinePlus