Intrapleural delivery of MSCs attenuates acute lung injury by paracrine/endocrine mechanism.
Bottom Line: MSCs intrapleural injection significantly improved LPS-induced lung histopathology compared with PBS-treated group at day 3.There was also a significant decrease in total cell counts and protein concentration in BALF at day 7 in the MSCs -treated rats compared to PBS control group.In conclusion, treatment with intrapleural MSCs administration markedly attenuates the severity of endotoxin-induced ALI.
Affiliation: Department of Pulmonary Medicine, Huadong Hospital, Shanghai Medical College, Fudan University, Shanghai, China.Show MeSH
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Mentions: We detected the levels of pro-inflammatory cytokine TNF-α and anti-inflammatory cytokine IL-10 in lung tissue and BALF by ELISA. The mRNA levels of these cytokines in lung tissue were also examined by real-time RT-PCR. At day 3, TNF-α in BALF was significantly reduced in the MSCs treatment group compared with that in PBS treatment group (P < 0.05) (Fig. 8). Similar results of TNF-α protein were observed in lung tissue. TNF-αmRNA expression in lung tissue increased 3 days after LPS exposure, although no significant difference between the MSCs- and PBS- treatment groups was observed at all time-points. At day 1, the level of IL-10 in lung tissue was significantly higher in the MSCs treatment group than that in the PBS treatment group (P < 0.05) (Fig. 8), same with the results of IL-10 mRNA expression (P < 0.05) (Fig. 8). LPS induced a significant increase of IL-10 in BALF at day 1, then gradually decreased to the normal levels at day 7. The levels of IL-10 protein in BALF showed no difference between the MSCs group and the PBS group.
Affiliation: Department of Pulmonary Medicine, Huadong Hospital, Shanghai Medical College, Fudan University, Shanghai, China.