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Intrapleural delivery of MSCs attenuates acute lung injury by paracrine/endocrine mechanism.

Qin ZH, Xu JF, Qu JM, Zhang J, Sai Y, Chen CM, Wu L, Yu L - J. Cell. Mol. Med. (2012)

Bottom Line: MSCs intrapleural injection significantly improved LPS-induced lung histopathology compared with PBS-treated group at day 3.There was also a significant decrease in total cell counts and protein concentration in BALF at day 7 in the MSCs -treated rats compared to PBS control group.In conclusion, treatment with intrapleural MSCs administration markedly attenuates the severity of endotoxin-induced ALI.

View Article: PubMed Central - PubMed

Affiliation: Department of Pulmonary Medicine, Huadong Hospital, Shanghai Medical College, Fudan University, Shanghai, China.

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Macroscopic observation of the BALF suggested an attenuated haemorrhage induced by LPS at day 3 in the MSCs treatment group. (A) Six specimens collected from PBS treatment group at day 3. (B) Six specimens collected from MSCs treatment group at day 3. Obviously, the two reddest BALF samples (black arrows) were from the PBS control group rather than from the MSCs treatment group.
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fig06: Macroscopic observation of the BALF suggested an attenuated haemorrhage induced by LPS at day 3 in the MSCs treatment group. (A) Six specimens collected from PBS treatment group at day 3. (B) Six specimens collected from MSCs treatment group at day 3. Obviously, the two reddest BALF samples (black arrows) were from the PBS control group rather than from the MSCs treatment group.

Mentions: Total protein concentration in the BALF, which reflects the extent of endothelial/epithelial permeability of the lung, was significantly increased in all rats even 7 days after LPS challenge. A significant decrease was observed at day 7 in MSCs treatment group compared with PBS treatment group (P < 0.05) (Fig. 5A). LPS challenge also significantly increased total cell count in BALF, including large number of red cells due to alveolar haemorrhage and neutrophils in the early day 1 and day 3 time-points. Total cell number in BALF was decreased due to MSCs treatment at day 7, compared with PBS treatment (P < 0.01) (Fig. 5B). The neutrophil counts between both groups were not significantly different at all three time-points (Fig. 5C). Macroscopic observation of the BALF at day 3 also suggested an attenuated haemorrhage induced by LPS in the MSCs treatment group (Fig. 6).


Intrapleural delivery of MSCs attenuates acute lung injury by paracrine/endocrine mechanism.

Qin ZH, Xu JF, Qu JM, Zhang J, Sai Y, Chen CM, Wu L, Yu L - J. Cell. Mol. Med. (2012)

Macroscopic observation of the BALF suggested an attenuated haemorrhage induced by LPS at day 3 in the MSCs treatment group. (A) Six specimens collected from PBS treatment group at day 3. (B) Six specimens collected from MSCs treatment group at day 3. Obviously, the two reddest BALF samples (black arrows) were from the PBS control group rather than from the MSCs treatment group.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4118243&req=5

fig06: Macroscopic observation of the BALF suggested an attenuated haemorrhage induced by LPS at day 3 in the MSCs treatment group. (A) Six specimens collected from PBS treatment group at day 3. (B) Six specimens collected from MSCs treatment group at day 3. Obviously, the two reddest BALF samples (black arrows) were from the PBS control group rather than from the MSCs treatment group.
Mentions: Total protein concentration in the BALF, which reflects the extent of endothelial/epithelial permeability of the lung, was significantly increased in all rats even 7 days after LPS challenge. A significant decrease was observed at day 7 in MSCs treatment group compared with PBS treatment group (P < 0.05) (Fig. 5A). LPS challenge also significantly increased total cell count in BALF, including large number of red cells due to alveolar haemorrhage and neutrophils in the early day 1 and day 3 time-points. Total cell number in BALF was decreased due to MSCs treatment at day 7, compared with PBS treatment (P < 0.01) (Fig. 5B). The neutrophil counts between both groups were not significantly different at all three time-points (Fig. 5C). Macroscopic observation of the BALF at day 3 also suggested an attenuated haemorrhage induced by LPS in the MSCs treatment group (Fig. 6).

Bottom Line: MSCs intrapleural injection significantly improved LPS-induced lung histopathology compared with PBS-treated group at day 3.There was also a significant decrease in total cell counts and protein concentration in BALF at day 7 in the MSCs -treated rats compared to PBS control group.In conclusion, treatment with intrapleural MSCs administration markedly attenuates the severity of endotoxin-induced ALI.

View Article: PubMed Central - PubMed

Affiliation: Department of Pulmonary Medicine, Huadong Hospital, Shanghai Medical College, Fudan University, Shanghai, China.

Show MeSH
Related in: MedlinePlus