Intrapleural delivery of MSCs attenuates acute lung injury by paracrine/endocrine mechanism.
Bottom Line: MSCs intrapleural injection significantly improved LPS-induced lung histopathology compared with PBS-treated group at day 3.There was also a significant decrease in total cell counts and protein concentration in BALF at day 7 in the MSCs -treated rats compared to PBS control group.In conclusion, treatment with intrapleural MSCs administration markedly attenuates the severity of endotoxin-induced ALI.
Affiliation: Department of Pulmonary Medicine, Huadong Hospital, Shanghai Medical College, Fudan University, Shanghai, China.Show MeSH
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Mentions: Total protein concentration in the BALF, which reflects the extent of endothelial/epithelial permeability of the lung, was significantly increased in all rats even 7 days after LPS challenge. A significant decrease was observed at day 7 in MSCs treatment group compared with PBS treatment group (P < 0.05) (Fig. 5A). LPS challenge also significantly increased total cell count in BALF, including large number of red cells due to alveolar haemorrhage and neutrophils in the early day 1 and day 3 time-points. Total cell number in BALF was decreased due to MSCs treatment at day 7, compared with PBS treatment (P < 0.01) (Fig. 5B). The neutrophil counts between both groups were not significantly different at all three time-points (Fig. 5C). Macroscopic observation of the BALF at day 3 also suggested an attenuated haemorrhage induced by LPS in the MSCs treatment group (Fig. 6).
Affiliation: Department of Pulmonary Medicine, Huadong Hospital, Shanghai Medical College, Fudan University, Shanghai, China.