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Intrapleural delivery of MSCs attenuates acute lung injury by paracrine/endocrine mechanism.

Qin ZH, Xu JF, Qu JM, Zhang J, Sai Y, Chen CM, Wu L, Yu L - J. Cell. Mol. Med. (2012)

Bottom Line: MSCs intrapleural injection significantly improved LPS-induced lung histopathology compared with PBS-treated group at day 3.There was also a significant decrease in total cell counts and protein concentration in BALF at day 7 in the MSCs -treated rats compared to PBS control group.In conclusion, treatment with intrapleural MSCs administration markedly attenuates the severity of endotoxin-induced ALI.

View Article: PubMed Central - PubMed

Affiliation: Department of Pulmonary Medicine, Huadong Hospital, Shanghai Medical College, Fudan University, Shanghai, China.

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Related in: MedlinePlus

No MSCs were found in the lung parenchyma of rats with LPS-induced ALI after cells were injected intrapleurally. (A) Surface of right lung. (B) Surface of mediastinal pleura. (C, D) Frozen sections of lung tissue. DAPI-marked MSCs masses were found on the surfaces of pleurae and the lung, whereas no fluorescent cells were found in the lung parenchyma (white arrows). All images, ×200 magnification.
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fig01: No MSCs were found in the lung parenchyma of rats with LPS-induced ALI after cells were injected intrapleurally. (A) Surface of right lung. (B) Surface of mediastinal pleura. (C, D) Frozen sections of lung tissue. DAPI-marked MSCs masses were found on the surfaces of pleurae and the lung, whereas no fluorescent cells were found in the lung parenchyma (white arrows). All images, ×200 magnification.

Mentions: The distribution of intrapleural administrated DAPI-marked MSCs in rats with ALI was found to be limited to the surface of pleurae and in the pleural cavity, no fluorescent cells were found in the lung parenchyma (Fig. 1), similar to the results observed with healthy rats in our previous study [23].


Intrapleural delivery of MSCs attenuates acute lung injury by paracrine/endocrine mechanism.

Qin ZH, Xu JF, Qu JM, Zhang J, Sai Y, Chen CM, Wu L, Yu L - J. Cell. Mol. Med. (2012)

No MSCs were found in the lung parenchyma of rats with LPS-induced ALI after cells were injected intrapleurally. (A) Surface of right lung. (B) Surface of mediastinal pleura. (C, D) Frozen sections of lung tissue. DAPI-marked MSCs masses were found on the surfaces of pleurae and the lung, whereas no fluorescent cells were found in the lung parenchyma (white arrows). All images, ×200 magnification.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4118243&req=5

fig01: No MSCs were found in the lung parenchyma of rats with LPS-induced ALI after cells were injected intrapleurally. (A) Surface of right lung. (B) Surface of mediastinal pleura. (C, D) Frozen sections of lung tissue. DAPI-marked MSCs masses were found on the surfaces of pleurae and the lung, whereas no fluorescent cells were found in the lung parenchyma (white arrows). All images, ×200 magnification.
Mentions: The distribution of intrapleural administrated DAPI-marked MSCs in rats with ALI was found to be limited to the surface of pleurae and in the pleural cavity, no fluorescent cells were found in the lung parenchyma (Fig. 1), similar to the results observed with healthy rats in our previous study [23].

Bottom Line: MSCs intrapleural injection significantly improved LPS-induced lung histopathology compared with PBS-treated group at day 3.There was also a significant decrease in total cell counts and protein concentration in BALF at day 7 in the MSCs -treated rats compared to PBS control group.In conclusion, treatment with intrapleural MSCs administration markedly attenuates the severity of endotoxin-induced ALI.

View Article: PubMed Central - PubMed

Affiliation: Department of Pulmonary Medicine, Huadong Hospital, Shanghai Medical College, Fudan University, Shanghai, China.

Show MeSH
Related in: MedlinePlus