CstF-64 supports pluripotency and regulates cell cycle progression in embryonic stem cells through histone 3' end processing.
Bottom Line: Similarly, the role of 3' end processing in regulation of ESC pluripotency and cell cycle is poorly understood.However, τCstF-64 only partially compensates for lost CstF-64 function, despite being recruited to the histone mRNA 3' end-processing complex.Reduction of τCstF-64 in CstF-64-deficient ESCs results in even greater levels of histone mRNA polyadenylation, suggesting that both CstF-64 and τCstF-64 function to inhibit polyadenylation of histone mRNAs.
Affiliation: Department of Cell Biology & Biochemistry, Texas Tech University Health Sciences Center, 3601 4th Street, Lubbock, TX 79430-6540, USA.Show MeSH
Related in: MedlinePlus
Mentions: Compared to wild type mouse ESCs (Figure 2A), the Cstf2E6 cells displayed a different phenotype that consisted of a flattened morphology and decreased propensity to grow in tightly packed clusters (Figure 2B). To assess whether these morphological differences were accompanied by changes in pluripotency markers, we stained wild type and Cstf2E6 ESCs with alkaline phosphatase, an enzyme that is not expressed in differentiated ESCs (37). Wild type ESCs displayed intense staining for alkaline phosphatase, while the Cstf2E6 cells displayed less staining, suggesting diminished pluripotency (Figure 2A, B). This reduction is similar to wild type ESCs grown in the absence of LIF for 96 h displayed reduced staining (Figure 2C).
Affiliation: Department of Cell Biology & Biochemistry, Texas Tech University Health Sciences Center, 3601 4th Street, Lubbock, TX 79430-6540, USA.