Structural studies of CNG repeats.
Bottom Line: Each homobasic pair is accommodated in the helical context in a unique manner, with consequences for the local helical parameters, solvent structure, electrostatic potential and potential to interact with ligands.The detailed three-dimensional profiles of RNA CNG repeats can be used in screening of compound libraries for potential therapeutics and in structure-based drug design.Here is a brief survey of the CNG structures published to date.
Affiliation: Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznan, Poland.Show MeSH
Related in: MedlinePlus
Mentions: Only one three-dimensional structure has been published of DNA containing CNG repeats (78). The solution structure of d(CCGCCG)2 was solved using NMR (PDB code 1noq). The double helix contained only G-C and C-G pairs. The C-C pairs were eliminated by strand slippage, which resulted in dangling cytosine residues at the 5′ site of each strand. In addition, both 4C residues bulged out causing deformation of the phosphate backbone (Figure 5). The structure shows similarity with the crystallographic models in that the unstable C-C pairs have been eliminated.
Affiliation: Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznan, Poland.