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Zaprinast and rolipram enhances spatial and emotional memory in the elevated plus maze and passive avoidance tests and diminishes exploratory activity in naive mice.

Akar F, Mutlu O, Komsuoglu Celikyurt I, Ulak G, Erden F, Bektas E, Tanyeri P - Med Sci Monit Basic Res (2014)

Bottom Line: Both zaprinast (10 mg/kg) and rolipram (0.1 mg/kg) significantly decreased the number of entries to new areas and time spent in new areas in the Hughes box test.Our study revealed that both zaprinast and rolipram enhanced spatial memory in EPM, while rolipram seemed to have more emotional memory-enhancing effects in the PA test compared to zaprinast.Both zaprinast and rolipram diminished exploratory activity in the Hughes box test, which can be attributed to the drugs' anxiogenic effects.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Medical Faculty, Kocaeli University, Kocaeli, Turkey.

ABSTRACT

Background: Phosphodiesterase (PDE) inhibitors in the central nervous system have been shown to stimulate neuronal functions and increase neurogenesis in Alzheimer disease (AD) patients.

Material/methods: The aim of this study was to investigate the effects of zaprinast, a PDE5 inhibitor, and rolipram, a PDE4 inhibitor, on learning and memory in elevated plus maze (EPM) and passive avoidance (PA) tests in naive mice. Male Balb-c mice received short-term treatment with zaprinast (3 and 10 mg/kg) and rolipram (0.05 and 0.1 mg/kg) before the acquisition trial of the EPM and PA tests. The exploratory activity of the animals was also investigated in the Hughes box test.

Results: Both zaprinast (10 mg/kg) and rolipram (0.1 mg/kg) significantly decreased second-day latency compared to the control group in the EPM test, while only rolipram (0.1 mg/kg) significantly increased second-day latency in the PA test. Both zaprinast (10 mg/kg) and rolipram (0.1 mg/kg) significantly decreased the number of entries to new areas and time spent in new areas in the Hughes box test.

Conclusions: Our study revealed that both zaprinast and rolipram enhanced spatial memory in EPM, while rolipram seemed to have more emotional memory-enhancing effects in the PA test compared to zaprinast. Both zaprinast and rolipram diminished exploratory activity in the Hughes box test, which can be attributed to the drugs' anxiogenic effects.

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Related in: MedlinePlus

Effect of zaprinast (3 and 10 mg/kg) and rolipram (0.05 and 0.1 mg/kg) administration on exploratory activity in free exploratory paradigm (Hughes Box) (n=6). Drugs were injected 60 and 30 min, respectively, prior to testing. The data are expressed as mean ±SEM values. a: total number of entries to novel side, b: total time spent in the novel side. * p<0.05, ** p<0.01 compared to the control group.
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f3-medscimonitbasicres-20-105: Effect of zaprinast (3 and 10 mg/kg) and rolipram (0.05 and 0.1 mg/kg) administration on exploratory activity in free exploratory paradigm (Hughes Box) (n=6). Drugs were injected 60 and 30 min, respectively, prior to testing. The data are expressed as mean ±SEM values. a: total number of entries to novel side, b: total time spent in the novel side. * p<0.05, ** p<0.01 compared to the control group.

Mentions: There was a significant difference between groups after evaluating the total number of entries to the novel side [F(4.29)=4.86, p=0.0049; Figure 3A] and total time spent in the novel side [F(4.29)=4.25, p=0.009, Figure 3B] in the Hughes box. Both zaprinast (10 mg/kg) and rolipram (0.1 mg/kg) significantly decreased entries to the novel side compared to the control group in the Hughes box when administered before the test (p<0.01 and p<0.05; respectively; Figure 3A). Zaprinast (3 and 10 mg/kg) and rolipram (0.1 mg/kg) also significantly shortened the time spent in the novel side in the Hughes box (p<0.05; Figure 3B).


Zaprinast and rolipram enhances spatial and emotional memory in the elevated plus maze and passive avoidance tests and diminishes exploratory activity in naive mice.

Akar F, Mutlu O, Komsuoglu Celikyurt I, Ulak G, Erden F, Bektas E, Tanyeri P - Med Sci Monit Basic Res (2014)

Effect of zaprinast (3 and 10 mg/kg) and rolipram (0.05 and 0.1 mg/kg) administration on exploratory activity in free exploratory paradigm (Hughes Box) (n=6). Drugs were injected 60 and 30 min, respectively, prior to testing. The data are expressed as mean ±SEM values. a: total number of entries to novel side, b: total time spent in the novel side. * p<0.05, ** p<0.01 compared to the control group.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4117679&req=5

f3-medscimonitbasicres-20-105: Effect of zaprinast (3 and 10 mg/kg) and rolipram (0.05 and 0.1 mg/kg) administration on exploratory activity in free exploratory paradigm (Hughes Box) (n=6). Drugs were injected 60 and 30 min, respectively, prior to testing. The data are expressed as mean ±SEM values. a: total number of entries to novel side, b: total time spent in the novel side. * p<0.05, ** p<0.01 compared to the control group.
Mentions: There was a significant difference between groups after evaluating the total number of entries to the novel side [F(4.29)=4.86, p=0.0049; Figure 3A] and total time spent in the novel side [F(4.29)=4.25, p=0.009, Figure 3B] in the Hughes box. Both zaprinast (10 mg/kg) and rolipram (0.1 mg/kg) significantly decreased entries to the novel side compared to the control group in the Hughes box when administered before the test (p<0.01 and p<0.05; respectively; Figure 3A). Zaprinast (3 and 10 mg/kg) and rolipram (0.1 mg/kg) also significantly shortened the time spent in the novel side in the Hughes box (p<0.05; Figure 3B).

Bottom Line: Both zaprinast (10 mg/kg) and rolipram (0.1 mg/kg) significantly decreased the number of entries to new areas and time spent in new areas in the Hughes box test.Our study revealed that both zaprinast and rolipram enhanced spatial memory in EPM, while rolipram seemed to have more emotional memory-enhancing effects in the PA test compared to zaprinast.Both zaprinast and rolipram diminished exploratory activity in the Hughes box test, which can be attributed to the drugs' anxiogenic effects.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Medical Faculty, Kocaeli University, Kocaeli, Turkey.

ABSTRACT

Background: Phosphodiesterase (PDE) inhibitors in the central nervous system have been shown to stimulate neuronal functions and increase neurogenesis in Alzheimer disease (AD) patients.

Material/methods: The aim of this study was to investigate the effects of zaprinast, a PDE5 inhibitor, and rolipram, a PDE4 inhibitor, on learning and memory in elevated plus maze (EPM) and passive avoidance (PA) tests in naive mice. Male Balb-c mice received short-term treatment with zaprinast (3 and 10 mg/kg) and rolipram (0.05 and 0.1 mg/kg) before the acquisition trial of the EPM and PA tests. The exploratory activity of the animals was also investigated in the Hughes box test.

Results: Both zaprinast (10 mg/kg) and rolipram (0.1 mg/kg) significantly decreased second-day latency compared to the control group in the EPM test, while only rolipram (0.1 mg/kg) significantly increased second-day latency in the PA test. Both zaprinast (10 mg/kg) and rolipram (0.1 mg/kg) significantly decreased the number of entries to new areas and time spent in new areas in the Hughes box test.

Conclusions: Our study revealed that both zaprinast and rolipram enhanced spatial memory in EPM, while rolipram seemed to have more emotional memory-enhancing effects in the PA test compared to zaprinast. Both zaprinast and rolipram diminished exploratory activity in the Hughes box test, which can be attributed to the drugs' anxiogenic effects.

Show MeSH
Related in: MedlinePlus