Calcium ions and osteoclastogenesis initiate the induction of bone formation by coral-derived macroporous constructs.
Bottom Line: Osteoclastic inhibition yielded minimal, if any, bone formation by induction. 7% HA/CC pre-loaded with the Ca(++) channel blocker verapamil hydrochloride strongly inhibited the induction of bone formation.Down-regulation of bone morphogenetic protein-2 (BMP-2) together with up-regulation of Noggin genes correlated with limited bone formation in 7% HA/CC pre-loaded with either verapamil or zoledronate, indicating that the induction of bone formation by coral-derived macroporous constructs is via the BMPs pathway.The spontaneous induction of bone formation is initiated by a local peak of Ca(++) activating stem cell differentiation and the induction of bone formation.
Affiliation: Bone Research Laboratory, School of Physiology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa.Show MeSH
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Mentions: By day 90, auto induced bone by untreated macroporous constructs increased significantly (Fig. 4), and remodelled in blocks of osteonic lamellar bone throughout the macroporous spaces (Fig. 5A–F) with osteocytes embedded within the newly formed matrix (Fig. 5G and H). Capillary sprouting was associated with osteoclastic activity remodelling the newly formed bone (Fig. 5H).
Affiliation: Bone Research Laboratory, School of Physiology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa.