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Calcium ions and osteoclastogenesis initiate the induction of bone formation by coral-derived macroporous constructs.

Klar RM, Duarte R, Dix-Peek T, Dickens C, Ferretti C, Ripamonti U - J. Cell. Mol. Med. (2013)

Bottom Line: Osteoclastic inhibition yielded minimal, if any, bone formation by induction. 7% HA/CC pre-loaded with the Ca(++) channel blocker verapamil hydrochloride strongly inhibited the induction of bone formation.Down-regulation of bone morphogenetic protein-2 (BMP-2) together with up-regulation of Noggin genes correlated with limited bone formation in 7% HA/CC pre-loaded with either verapamil or zoledronate, indicating that the induction of bone formation by coral-derived macroporous constructs is via the BMPs pathway.The spontaneous induction of bone formation is initiated by a local peak of Ca(++) activating stem cell differentiation and the induction of bone formation.

View Article: PubMed Central - PubMed

Affiliation: Bone Research Laboratory, School of Physiology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa.

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Untreated control 7% HA/CC macroporous constructs on day 90. ‘Spontaneous’ and ‘intrinsic’ bone differentiation and remodelling in untreated control 7% HA/CC biomimetic constructs harvested on day 90 after intramuscular heterotopic implantation. (A–D) Low power views showing tissue patterning, remodelling of collagenous condensations and bone formation by induction within selected macroporous spaces. (E and F) High power views of previous sections (A and B) showing the induction of bone formation (dark blue arrows) supported by a pronounced vascular invasion across the macroporous spaces. (G and H) There is prominent remodelling with osteoclastogenesis (magenta arrows) of the newly formed corticalized lamellar bone (dark blue arrows). Note the intimacy of vascular invasion with newly differentiated large multinucleated osteoclastic cells within the remodelled bone matrix (white arrows). Decalcified sections cut at 4 μm stained with Goldner's trichrome.
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fig05: Untreated control 7% HA/CC macroporous constructs on day 90. ‘Spontaneous’ and ‘intrinsic’ bone differentiation and remodelling in untreated control 7% HA/CC biomimetic constructs harvested on day 90 after intramuscular heterotopic implantation. (A–D) Low power views showing tissue patterning, remodelling of collagenous condensations and bone formation by induction within selected macroporous spaces. (E and F) High power views of previous sections (A and B) showing the induction of bone formation (dark blue arrows) supported by a pronounced vascular invasion across the macroporous spaces. (G and H) There is prominent remodelling with osteoclastogenesis (magenta arrows) of the newly formed corticalized lamellar bone (dark blue arrows). Note the intimacy of vascular invasion with newly differentiated large multinucleated osteoclastic cells within the remodelled bone matrix (white arrows). Decalcified sections cut at 4 μm stained with Goldner's trichrome.

Mentions: By day 90, auto induced bone by untreated macroporous constructs increased significantly (Fig. 4), and remodelled in blocks of osteonic lamellar bone throughout the macroporous spaces (Fig. 5A–F) with osteocytes embedded within the newly formed matrix (Fig. 5G and H). Capillary sprouting was associated with osteoclastic activity remodelling the newly formed bone (Fig. 5H).


Calcium ions and osteoclastogenesis initiate the induction of bone formation by coral-derived macroporous constructs.

Klar RM, Duarte R, Dix-Peek T, Dickens C, Ferretti C, Ripamonti U - J. Cell. Mol. Med. (2013)

Untreated control 7% HA/CC macroporous constructs on day 90. ‘Spontaneous’ and ‘intrinsic’ bone differentiation and remodelling in untreated control 7% HA/CC biomimetic constructs harvested on day 90 after intramuscular heterotopic implantation. (A–D) Low power views showing tissue patterning, remodelling of collagenous condensations and bone formation by induction within selected macroporous spaces. (E and F) High power views of previous sections (A and B) showing the induction of bone formation (dark blue arrows) supported by a pronounced vascular invasion across the macroporous spaces. (G and H) There is prominent remodelling with osteoclastogenesis (magenta arrows) of the newly formed corticalized lamellar bone (dark blue arrows). Note the intimacy of vascular invasion with newly differentiated large multinucleated osteoclastic cells within the remodelled bone matrix (white arrows). Decalcified sections cut at 4 μm stained with Goldner's trichrome.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4117557&req=5

fig05: Untreated control 7% HA/CC macroporous constructs on day 90. ‘Spontaneous’ and ‘intrinsic’ bone differentiation and remodelling in untreated control 7% HA/CC biomimetic constructs harvested on day 90 after intramuscular heterotopic implantation. (A–D) Low power views showing tissue patterning, remodelling of collagenous condensations and bone formation by induction within selected macroporous spaces. (E and F) High power views of previous sections (A and B) showing the induction of bone formation (dark blue arrows) supported by a pronounced vascular invasion across the macroporous spaces. (G and H) There is prominent remodelling with osteoclastogenesis (magenta arrows) of the newly formed corticalized lamellar bone (dark blue arrows). Note the intimacy of vascular invasion with newly differentiated large multinucleated osteoclastic cells within the remodelled bone matrix (white arrows). Decalcified sections cut at 4 μm stained with Goldner's trichrome.
Mentions: By day 90, auto induced bone by untreated macroporous constructs increased significantly (Fig. 4), and remodelled in blocks of osteonic lamellar bone throughout the macroporous spaces (Fig. 5A–F) with osteocytes embedded within the newly formed matrix (Fig. 5G and H). Capillary sprouting was associated with osteoclastic activity remodelling the newly formed bone (Fig. 5H).

Bottom Line: Osteoclastic inhibition yielded minimal, if any, bone formation by induction. 7% HA/CC pre-loaded with the Ca(++) channel blocker verapamil hydrochloride strongly inhibited the induction of bone formation.Down-regulation of bone morphogenetic protein-2 (BMP-2) together with up-regulation of Noggin genes correlated with limited bone formation in 7% HA/CC pre-loaded with either verapamil or zoledronate, indicating that the induction of bone formation by coral-derived macroporous constructs is via the BMPs pathway.The spontaneous induction of bone formation is initiated by a local peak of Ca(++) activating stem cell differentiation and the induction of bone formation.

View Article: PubMed Central - PubMed

Affiliation: Bone Research Laboratory, School of Physiology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa.

Show MeSH
Related in: MedlinePlus