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Calcium ions and osteoclastogenesis initiate the induction of bone formation by coral-derived macroporous constructs.

Klar RM, Duarte R, Dix-Peek T, Dickens C, Ferretti C, Ripamonti U - J. Cell. Mol. Med. (2013)

Bottom Line: Generated tissues on days 15, 60 and 90 were analysed by histomorphometry and qRT-PCR.On day 15, up-regulation of type IV collagen characterized all the implanted constructs correlating with vascular invasion.Zoledronate-treated specimens showed an important delay in tissue patterning and morphogenesis with limited bone formation.

View Article: PubMed Central - PubMed

Affiliation: Bone Research Laboratory, School of Physiology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa.

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Relative change in gene expression of BMP-2, collagen type IV and Noggin at days 15 (15d), 60 (60d) and 90 (90d) within untreated 7% HA/CC control (A), verapamil (B) and zoledronate-pre-treated 7% HA/CC (C) implanted within the rectus abdominis muscle of six adult Chacma baboons Papio ursinus (**p < 0.05, ***p < 0.01).
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fig03: Relative change in gene expression of BMP-2, collagen type IV and Noggin at days 15 (15d), 60 (60d) and 90 (90d) within untreated 7% HA/CC control (A), verapamil (B) and zoledronate-pre-treated 7% HA/CC (C) implanted within the rectus abdominis muscle of six adult Chacma baboons Papio ursinus (**p < 0.05, ***p < 0.01).

Mentions: Angiogenesis correlated with type IV collagen up-regulation irrespective of the treatment modalities (Fig. 3). 7% HA/CC untreated controls showed pronounced capillary sprouting and vascular invasion within the peripheral macroporous spaces (Fig. 2A–C). Morphogenesis and tissue patterning of collagenous condensations were well advanced on day 15 (Fig. 2B and C) with remodelling and further development on day 60 (Fig. 2D–F). Areas with prominent angiogenesis showed a pronounced capillary sprouting and invasion with hypercellularity (Fig. 2B and C). Paravascular pericytic cells showed enlarged hyper-chromatic nuclei with several cells migrating between the vascular compartment and the paravascular extracellular matrix (Fig. 2C).


Calcium ions and osteoclastogenesis initiate the induction of bone formation by coral-derived macroporous constructs.

Klar RM, Duarte R, Dix-Peek T, Dickens C, Ferretti C, Ripamonti U - J. Cell. Mol. Med. (2013)

Relative change in gene expression of BMP-2, collagen type IV and Noggin at days 15 (15d), 60 (60d) and 90 (90d) within untreated 7% HA/CC control (A), verapamil (B) and zoledronate-pre-treated 7% HA/CC (C) implanted within the rectus abdominis muscle of six adult Chacma baboons Papio ursinus (**p < 0.05, ***p < 0.01).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4117557&req=5

fig03: Relative change in gene expression of BMP-2, collagen type IV and Noggin at days 15 (15d), 60 (60d) and 90 (90d) within untreated 7% HA/CC control (A), verapamil (B) and zoledronate-pre-treated 7% HA/CC (C) implanted within the rectus abdominis muscle of six adult Chacma baboons Papio ursinus (**p < 0.05, ***p < 0.01).
Mentions: Angiogenesis correlated with type IV collagen up-regulation irrespective of the treatment modalities (Fig. 3). 7% HA/CC untreated controls showed pronounced capillary sprouting and vascular invasion within the peripheral macroporous spaces (Fig. 2A–C). Morphogenesis and tissue patterning of collagenous condensations were well advanced on day 15 (Fig. 2B and C) with remodelling and further development on day 60 (Fig. 2D–F). Areas with prominent angiogenesis showed a pronounced capillary sprouting and invasion with hypercellularity (Fig. 2B and C). Paravascular pericytic cells showed enlarged hyper-chromatic nuclei with several cells migrating between the vascular compartment and the paravascular extracellular matrix (Fig. 2C).

Bottom Line: Generated tissues on days 15, 60 and 90 were analysed by histomorphometry and qRT-PCR.On day 15, up-regulation of type IV collagen characterized all the implanted constructs correlating with vascular invasion.Zoledronate-treated specimens showed an important delay in tissue patterning and morphogenesis with limited bone formation.

View Article: PubMed Central - PubMed

Affiliation: Bone Research Laboratory, School of Physiology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa.

Show MeSH
Related in: MedlinePlus