Calcium ions and osteoclastogenesis initiate the induction of bone formation by coral-derived macroporous constructs.
Bottom Line: Osteoclastic inhibition yielded minimal, if any, bone formation by induction. 7% HA/CC pre-loaded with the Ca(++) channel blocker verapamil hydrochloride strongly inhibited the induction of bone formation.Down-regulation of bone morphogenetic protein-2 (BMP-2) together with up-regulation of Noggin genes correlated with limited bone formation in 7% HA/CC pre-loaded with either verapamil or zoledronate, indicating that the induction of bone formation by coral-derived macroporous constructs is via the BMPs pathway.The spontaneous induction of bone formation is initiated by a local peak of Ca(++) activating stem cell differentiation and the induction of bone formation.
Affiliation: Bone Research Laboratory, School of Physiology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa.Show MeSH
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Mentions: Digital iconographic images of 7% HA/CC untreated control are presented in Figure 2, at 15 and 60 days, and Figure 5, at 90 days. At harvest, all implants were firmly attached to the ventral fascia and the surrounding rectus abdominis muscle. On day 15, the most peripheral macroporous spaces were invaded by a highly vascular connective tissue matrix (Fig. 2A–C). Vascular invasion and capillary sprouting was pronounced in all treatment modalities. Previous experiments in the non-human primate P. ursinus have shown that the specific geometry and surface characteristics of the coral-derived substratum are conducive to rapid vessels ingrowths’ and capillary sprouting within the early mesenchyme penetrating the macroporous spaces 3.
Affiliation: Bone Research Laboratory, School of Physiology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa.