Calcium ions and osteoclastogenesis initiate the induction of bone formation by coral-derived macroporous constructs.
Bottom Line: Generated tissues on days 15, 60 and 90 were analysed by histomorphometry and qRT-PCR.On day 15, up-regulation of type IV collagen characterized all the implanted constructs correlating with vascular invasion.Zoledronate-treated specimens showed an important delay in tissue patterning and morphogenesis with limited bone formation.
Affiliation: Bone Research Laboratory, School of Physiology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa.Show MeSH
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Mentions: Digital iconographic images of 7% HA/CC untreated control are presented in Figure 2, at 15 and 60 days, and Figure 5, at 90 days. At harvest, all implants were firmly attached to the ventral fascia and the surrounding rectus abdominis muscle. On day 15, the most peripheral macroporous spaces were invaded by a highly vascular connective tissue matrix (Fig. 2A–C). Vascular invasion and capillary sprouting was pronounced in all treatment modalities. Previous experiments in the non-human primate P. ursinus have shown that the specific geometry and surface characteristics of the coral-derived substratum are conducive to rapid vessels ingrowths’ and capillary sprouting within the early mesenchyme penetrating the macroporous spaces 3.
Affiliation: Bone Research Laboratory, School of Physiology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa.