Limits...
Expression of the prostaglandin F synthase AKR1B1 and the prostaglandin transporter SLCO2A1 in human fetal membranes in relation to spontaneous term and preterm labor.

Alzamil HA, Pawade J, Fortier MA, Bernal AL - Front Physiol (2014)

Bottom Line: Using fetal membranes explants we tested the effect of cytokines (interleukin-1 and tumor necrosis factor alpha) on PG production and the concomitant changes in cyclooxygenase-2 (PTGS2), AKR1B1 and SLCO2A1 expression.The amnion is more responsive than choriodecidua to stimulation with pro-inflammatory cytokines.The mechanisms of term and preterm labor are different.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, King Saud University Riyadh, Saudi Arabia.

ABSTRACT

Background: Human labor is a complex series of cellular and molecular events that occur at the materno-fetal and uterine levels. Many hypotheses have been proposed for the initiation of human labor, one hypothesis suggests that maturation of the fetus releases a signal in the amniotic fluid that will be transmitted to myometrium via the fetal membranes and initiate uterine contractions. There is strong evidence that prostaglandins (PGs) play a central role in initiation and progression of human labor.

Objectives: In this study we intended to investigate the expression of prostaglandin F synthase and the prostaglandin transporter in the human fetal membranes and to explore the relationship between cytokines and PGs in the mechanism of human labor.

Methods: We used fetal membranes obtained before labor at term and after spontaneous labor at term or preterm to identify the changes in prostaglandin F synthase (AKR1B1) and human prostaglandin transporter (SLCO2A1) proteins in relation to parturition. Using fetal membranes explants we tested the effect of cytokines (interleukin-1 and tumor necrosis factor alpha) on PG production and the concomitant changes in cyclooxygenase-2 (PTGS2), AKR1B1 and SLCO2A1 expression.

Results: Expression of PTGS2 and AKR1B1 was upregulated in the fetal membranes in association with term labor while SLCO2A1 was downregulated with advancing gestation and during term labor. Before labor, IL-1 increased the expression of PTGS2, however during labor TNF upregulated PTGS2 and AKR1B1 proteins.

Conclusions: The prostaglandin F synthase AKR1B1 is upregulated while prostaglandin transporter is downregulated during term labor. The amnion is more responsive than choriodecidua to stimulation with pro-inflammatory cytokines. The mechanisms of term and preterm labor are different.

No MeSH data available.


Related in: MedlinePlus

Immunohistochemistry staining of term decidua basalis (A), chorionic villi (B) and fetal membranes (C) with AKR1B1 antibody (D–F), SLCO2A1 antibody (G–I), and PTGS2 antibody (J–L). Primary antibodies were omitted in (A–C) which served as controls. DC, decidual cells; Syn, syncytiotrophoblast; Cyt, cytotrophoblast; IVS, intervillous space; De, decidua parietalis; Ch, chorion; and A, amnion. Bar = 100 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4115629&req=5

Figure 1: Immunohistochemistry staining of term decidua basalis (A), chorionic villi (B) and fetal membranes (C) with AKR1B1 antibody (D–F), SLCO2A1 antibody (G–I), and PTGS2 antibody (J–L). Primary antibodies were omitted in (A–C) which served as controls. DC, decidual cells; Syn, syncytiotrophoblast; Cyt, cytotrophoblast; IVS, intervillous space; De, decidua parietalis; Ch, chorion; and A, amnion. Bar = 100 μm.

Mentions: AKR1B1 and SLCO2A1 proteins were clearly expressed in decidual cells (Figures 1A–L). In decidual cytoplasm there was uneven distribution of AKR1B1 protein (Figure 1A) while the surrounding connective tissue appeared negative (Figure 1D). AKR1B1 protein was identified in the chorionic villi, in which the staining was strong in the cytoplasm of syncytiotrophoblasts while cytotrophoblasts and villous stroma were negative (Figure 1E). The SLCO2A1 protein was localized in decidual cytoplasm with no staining in the connective tissue (Figure 1G). The syncytiotrophoblasts in the chorionic villi showed weak staining in their cytoplasm and the cytotrophoblasts did not show any staining with SLCO2A1 antibodies (Figure 1H).


Expression of the prostaglandin F synthase AKR1B1 and the prostaglandin transporter SLCO2A1 in human fetal membranes in relation to spontaneous term and preterm labor.

Alzamil HA, Pawade J, Fortier MA, Bernal AL - Front Physiol (2014)

Immunohistochemistry staining of term decidua basalis (A), chorionic villi (B) and fetal membranes (C) with AKR1B1 antibody (D–F), SLCO2A1 antibody (G–I), and PTGS2 antibody (J–L). Primary antibodies were omitted in (A–C) which served as controls. DC, decidual cells; Syn, syncytiotrophoblast; Cyt, cytotrophoblast; IVS, intervillous space; De, decidua parietalis; Ch, chorion; and A, amnion. Bar = 100 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4115629&req=5

Figure 1: Immunohistochemistry staining of term decidua basalis (A), chorionic villi (B) and fetal membranes (C) with AKR1B1 antibody (D–F), SLCO2A1 antibody (G–I), and PTGS2 antibody (J–L). Primary antibodies were omitted in (A–C) which served as controls. DC, decidual cells; Syn, syncytiotrophoblast; Cyt, cytotrophoblast; IVS, intervillous space; De, decidua parietalis; Ch, chorion; and A, amnion. Bar = 100 μm.
Mentions: AKR1B1 and SLCO2A1 proteins were clearly expressed in decidual cells (Figures 1A–L). In decidual cytoplasm there was uneven distribution of AKR1B1 protein (Figure 1A) while the surrounding connective tissue appeared negative (Figure 1D). AKR1B1 protein was identified in the chorionic villi, in which the staining was strong in the cytoplasm of syncytiotrophoblasts while cytotrophoblasts and villous stroma were negative (Figure 1E). The SLCO2A1 protein was localized in decidual cytoplasm with no staining in the connective tissue (Figure 1G). The syncytiotrophoblasts in the chorionic villi showed weak staining in their cytoplasm and the cytotrophoblasts did not show any staining with SLCO2A1 antibodies (Figure 1H).

Bottom Line: Using fetal membranes explants we tested the effect of cytokines (interleukin-1 and tumor necrosis factor alpha) on PG production and the concomitant changes in cyclooxygenase-2 (PTGS2), AKR1B1 and SLCO2A1 expression.The amnion is more responsive than choriodecidua to stimulation with pro-inflammatory cytokines.The mechanisms of term and preterm labor are different.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, King Saud University Riyadh, Saudi Arabia.

ABSTRACT

Background: Human labor is a complex series of cellular and molecular events that occur at the materno-fetal and uterine levels. Many hypotheses have been proposed for the initiation of human labor, one hypothesis suggests that maturation of the fetus releases a signal in the amniotic fluid that will be transmitted to myometrium via the fetal membranes and initiate uterine contractions. There is strong evidence that prostaglandins (PGs) play a central role in initiation and progression of human labor.

Objectives: In this study we intended to investigate the expression of prostaglandin F synthase and the prostaglandin transporter in the human fetal membranes and to explore the relationship between cytokines and PGs in the mechanism of human labor.

Methods: We used fetal membranes obtained before labor at term and after spontaneous labor at term or preterm to identify the changes in prostaglandin F synthase (AKR1B1) and human prostaglandin transporter (SLCO2A1) proteins in relation to parturition. Using fetal membranes explants we tested the effect of cytokines (interleukin-1 and tumor necrosis factor alpha) on PG production and the concomitant changes in cyclooxygenase-2 (PTGS2), AKR1B1 and SLCO2A1 expression.

Results: Expression of PTGS2 and AKR1B1 was upregulated in the fetal membranes in association with term labor while SLCO2A1 was downregulated with advancing gestation and during term labor. Before labor, IL-1 increased the expression of PTGS2, however during labor TNF upregulated PTGS2 and AKR1B1 proteins.

Conclusions: The prostaglandin F synthase AKR1B1 is upregulated while prostaglandin transporter is downregulated during term labor. The amnion is more responsive than choriodecidua to stimulation with pro-inflammatory cytokines. The mechanisms of term and preterm labor are different.

No MeSH data available.


Related in: MedlinePlus