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Phytophthora ×stagnum nothosp. nov., a new hybrid from irrigation reservoirs at ornamental plant nurseries in Virginia.

Yang X, Richardson PA, Hong C - PLoS ONE (2014)

Bottom Line: It also produces both ornamented and relatively smooth-walled oogonia.Ornamented oogonia are another important diagnostic character of P. mississippiae.The relatively smooth-walled oogonia may be indicative of oogonial character of P. taxon PgChlamydo.

View Article: PubMed Central - PubMed

Affiliation: Hampton Roads Agricultural Research and Extension Center, Virginia Tech, Virginia Beach, Virginia, United States of America.

ABSTRACT
A novel Phytophthora species was frequently recovered from irrigation reservoirs at several ornamental plant production facilities in eastern Virginia. Initial sequencing of the internal transcribed spacer (ITS) region of this species generated unreadable sequences due to continual polymorphic positions. Cloning and sequencing the ITS region as well as sequencing the mitochondrially encoded cytochrome c oxidase 1 and beta-tubulin genes revealed that it is a hybrid between P. taxon PgChlamydo as its paternal parent and an unknown species genetically close to P. mississippiae as its maternal parent. This hybrid has some diagnostic morphological features of P. taxon PgChlamydo and P. mississippiae. It produces catenulate hyphal swellings, characteristic of P. mississippiae, and chlamydospores, typical of P. taxon PgChlamydo. It also produces both ornamented and relatively smooth-walled oogonia. Ornamented oogonia are another important diagnostic character of P. mississippiae. The relatively smooth-walled oogonia may be indicative of oogonial character of P. taxon PgChlamydo. The new hybrid is described here as Phytophthora ×stagnum.

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Related in: MedlinePlus

Average daily radial growth of Phytophthora ×stagnum representative isolates in carrot agar (CA) and 20% clarified V8 juice agar (CV8A) over an 8-days period.
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pone-0103450-g005: Average daily radial growth of Phytophthora ×stagnum representative isolates in carrot agar (CA) and 20% clarified V8 juice agar (CV8A) over an 8-days period.

Mentions: Radial growth rates were similar among four representative isolates (P = 0.71) and between two cardinal temperature tests (P = 0.74). Thus, data from the repeated tests were pooled and averages were plotted against temperature (Figure 5). The optimum temperature for the vegetative growth of P. ×stagnum in both media was 25°C. It also grew well at 30°C on both media. Limited growth occurred at 5 and 35°C. No growth was observed at 40°C.


Phytophthora ×stagnum nothosp. nov., a new hybrid from irrigation reservoirs at ornamental plant nurseries in Virginia.

Yang X, Richardson PA, Hong C - PLoS ONE (2014)

Average daily radial growth of Phytophthora ×stagnum representative isolates in carrot agar (CA) and 20% clarified V8 juice agar (CV8A) over an 8-days period.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4114803&req=5

pone-0103450-g005: Average daily radial growth of Phytophthora ×stagnum representative isolates in carrot agar (CA) and 20% clarified V8 juice agar (CV8A) over an 8-days period.
Mentions: Radial growth rates were similar among four representative isolates (P = 0.71) and between two cardinal temperature tests (P = 0.74). Thus, data from the repeated tests were pooled and averages were plotted against temperature (Figure 5). The optimum temperature for the vegetative growth of P. ×stagnum in both media was 25°C. It also grew well at 30°C on both media. Limited growth occurred at 5 and 35°C. No growth was observed at 40°C.

Bottom Line: It also produces both ornamented and relatively smooth-walled oogonia.Ornamented oogonia are another important diagnostic character of P. mississippiae.The relatively smooth-walled oogonia may be indicative of oogonial character of P. taxon PgChlamydo.

View Article: PubMed Central - PubMed

Affiliation: Hampton Roads Agricultural Research and Extension Center, Virginia Tech, Virginia Beach, Virginia, United States of America.

ABSTRACT
A novel Phytophthora species was frequently recovered from irrigation reservoirs at several ornamental plant production facilities in eastern Virginia. Initial sequencing of the internal transcribed spacer (ITS) region of this species generated unreadable sequences due to continual polymorphic positions. Cloning and sequencing the ITS region as well as sequencing the mitochondrially encoded cytochrome c oxidase 1 and beta-tubulin genes revealed that it is a hybrid between P. taxon PgChlamydo as its paternal parent and an unknown species genetically close to P. mississippiae as its maternal parent. This hybrid has some diagnostic morphological features of P. taxon PgChlamydo and P. mississippiae. It produces catenulate hyphal swellings, characteristic of P. mississippiae, and chlamydospores, typical of P. taxon PgChlamydo. It also produces both ornamented and relatively smooth-walled oogonia. Ornamented oogonia are another important diagnostic character of P. mississippiae. The relatively smooth-walled oogonia may be indicative of oogonial character of P. taxon PgChlamydo. The new hybrid is described here as Phytophthora ×stagnum.

Show MeSH
Related in: MedlinePlus