Limits...
Phytophthora ×stagnum nothosp. nov., a new hybrid from irrigation reservoirs at ornamental plant nurseries in Virginia.

Yang X, Richardson PA, Hong C - PLoS ONE (2014)

Bottom Line: It also produces both ornamented and relatively smooth-walled oogonia.Ornamented oogonia are another important diagnostic character of P. mississippiae.The relatively smooth-walled oogonia may be indicative of oogonial character of P. taxon PgChlamydo.

View Article: PubMed Central - PubMed

Affiliation: Hampton Roads Agricultural Research and Extension Center, Virginia Tech, Virginia Beach, Virginia, United States of America.

ABSTRACT
A novel Phytophthora species was frequently recovered from irrigation reservoirs at several ornamental plant production facilities in eastern Virginia. Initial sequencing of the internal transcribed spacer (ITS) region of this species generated unreadable sequences due to continual polymorphic positions. Cloning and sequencing the ITS region as well as sequencing the mitochondrially encoded cytochrome c oxidase 1 and beta-tubulin genes revealed that it is a hybrid between P. taxon PgChlamydo as its paternal parent and an unknown species genetically close to P. mississippiae as its maternal parent. This hybrid has some diagnostic morphological features of P. taxon PgChlamydo and P. mississippiae. It produces catenulate hyphal swellings, characteristic of P. mississippiae, and chlamydospores, typical of P. taxon PgChlamydo. It also produces both ornamented and relatively smooth-walled oogonia. Ornamented oogonia are another important diagnostic character of P. mississippiae. The relatively smooth-walled oogonia may be indicative of oogonial character of P. taxon PgChlamydo. The new hybrid is described here as Phytophthora ×stagnum.

Show MeSH

Related in: MedlinePlus

Beta-tubulin sequence alignment of Phytophthora ×stagnum, P. mississippiae and P. taxon PgChlamydo.Position numbers are given based on the alignment. Shading color: green indicates ambiguous sequences of polymorphic positions; yellow indicates sequences of the paternal parent P. taxon PgChlamydo; blue indicates sequences of the putative maternal parent. aY = T and C; S = G and C; M = A and C; R = G and A.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4114803&req=5

pone-0103450-g003: Beta-tubulin sequence alignment of Phytophthora ×stagnum, P. mississippiae and P. taxon PgChlamydo.Position numbers are given based on the alignment. Shading color: green indicates ambiguous sequences of polymorphic positions; yellow indicates sequences of the paternal parent P. taxon PgChlamydo; blue indicates sequences of the putative maternal parent. aY = T and C; S = G and C; M = A and C; R = G and A.

Mentions: Isolates 36H8, 36J7, and 44F9 resulted in an identical 1124-bp beta-tubulin sequence with 26 polymorphic positions (Figure 3). Isolate 43F3 also produced a 1124-bp sequence with 24 of the same 26 polymorphic positions. In spite of the polymorphic positions, beta-tubulin sequences of P. ×stagnum are identical to that of P. taxon PgChlamydo and 11 bp different from that of P. mississippiae (Figure 3). Sequences of P. taxon PgChlamydo and P. mississippiae are distinct and both occur at 18 of the 26 polymorphic positions of P. ×stagnum containing ambiguous sequences such as positions 99, 102, and 261 (Figure 3). At the other eight polymorphic positions such as positions 93, 306, and 450, both species share the same sequences which also occur as one of the ambiguous polymorphic sequences of P. ×stagnum (Figure 3). Putative sequences of the maternal parent of P. ×stagnum are shown in Figure 3. The maternal parent is approximately 21 bp different from P. mississippiae in beta-tubulin sequence.


Phytophthora ×stagnum nothosp. nov., a new hybrid from irrigation reservoirs at ornamental plant nurseries in Virginia.

Yang X, Richardson PA, Hong C - PLoS ONE (2014)

Beta-tubulin sequence alignment of Phytophthora ×stagnum, P. mississippiae and P. taxon PgChlamydo.Position numbers are given based on the alignment. Shading color: green indicates ambiguous sequences of polymorphic positions; yellow indicates sequences of the paternal parent P. taxon PgChlamydo; blue indicates sequences of the putative maternal parent. aY = T and C; S = G and C; M = A and C; R = G and A.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4114803&req=5

pone-0103450-g003: Beta-tubulin sequence alignment of Phytophthora ×stagnum, P. mississippiae and P. taxon PgChlamydo.Position numbers are given based on the alignment. Shading color: green indicates ambiguous sequences of polymorphic positions; yellow indicates sequences of the paternal parent P. taxon PgChlamydo; blue indicates sequences of the putative maternal parent. aY = T and C; S = G and C; M = A and C; R = G and A.
Mentions: Isolates 36H8, 36J7, and 44F9 resulted in an identical 1124-bp beta-tubulin sequence with 26 polymorphic positions (Figure 3). Isolate 43F3 also produced a 1124-bp sequence with 24 of the same 26 polymorphic positions. In spite of the polymorphic positions, beta-tubulin sequences of P. ×stagnum are identical to that of P. taxon PgChlamydo and 11 bp different from that of P. mississippiae (Figure 3). Sequences of P. taxon PgChlamydo and P. mississippiae are distinct and both occur at 18 of the 26 polymorphic positions of P. ×stagnum containing ambiguous sequences such as positions 99, 102, and 261 (Figure 3). At the other eight polymorphic positions such as positions 93, 306, and 450, both species share the same sequences which also occur as one of the ambiguous polymorphic sequences of P. ×stagnum (Figure 3). Putative sequences of the maternal parent of P. ×stagnum are shown in Figure 3. The maternal parent is approximately 21 bp different from P. mississippiae in beta-tubulin sequence.

Bottom Line: It also produces both ornamented and relatively smooth-walled oogonia.Ornamented oogonia are another important diagnostic character of P. mississippiae.The relatively smooth-walled oogonia may be indicative of oogonial character of P. taxon PgChlamydo.

View Article: PubMed Central - PubMed

Affiliation: Hampton Roads Agricultural Research and Extension Center, Virginia Tech, Virginia Beach, Virginia, United States of America.

ABSTRACT
A novel Phytophthora species was frequently recovered from irrigation reservoirs at several ornamental plant production facilities in eastern Virginia. Initial sequencing of the internal transcribed spacer (ITS) region of this species generated unreadable sequences due to continual polymorphic positions. Cloning and sequencing the ITS region as well as sequencing the mitochondrially encoded cytochrome c oxidase 1 and beta-tubulin genes revealed that it is a hybrid between P. taxon PgChlamydo as its paternal parent and an unknown species genetically close to P. mississippiae as its maternal parent. This hybrid has some diagnostic morphological features of P. taxon PgChlamydo and P. mississippiae. It produces catenulate hyphal swellings, characteristic of P. mississippiae, and chlamydospores, typical of P. taxon PgChlamydo. It also produces both ornamented and relatively smooth-walled oogonia. Ornamented oogonia are another important diagnostic character of P. mississippiae. The relatively smooth-walled oogonia may be indicative of oogonial character of P. taxon PgChlamydo. The new hybrid is described here as Phytophthora ×stagnum.

Show MeSH
Related in: MedlinePlus