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Differential expression of heat shock transcription factors and heat shock proteins after acute and chronic heat stress in laying chickens (Gallus gallus).

Xie J, Tang L, Lu L, Zhang L, Xi L, Liu HC, Odle J, Luo X - PLoS ONE (2014)

Bottom Line: Tissue-specific responses to thermal challenge were also found among heart, liver and muscle.HSP90 expression was increased by chronic thermal treatment (P<0.05).The differential expressions of HSF and HSP genes in different tissues of laying broiler breeder chickens suggested that anti-heat stress mechanisms might be provoked more profoundly in the heart, by which the muscle was least protected during heat stress.

View Article: PubMed Central - PubMed

Affiliation: Mineral Nutrition Research Division, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, People's Republic of China.

ABSTRACT
Heat stress due to high environmental temperature negatively influences animal performances. To better understand the biological impact of heat stress, laying broiler breeder chickens were subjected either to acute (step-wisely increasing temperature from 21 to 35°C within 24 hours) or chronic (32°C for 8 weeks) high temperature exposure. High temperature challenges significantly elevated body temperature of experimental birds (P<0.05). However, oxidation status of lipid and protein and expression of heat shock transcription factors (HSFs) and heat shock proteins (HSPs) 70 and 90 were differently affected by acute and chronic treatment. Tissue-specific responses to thermal challenge were also found among heart, liver and muscle. In the heart, acute heat challenge affected lipid oxidation (P = 0.05) and gene expression of all 4 HSF gene expression was upregulated (P<0.05). During chronic heat treatment, the HSP 70 mRNA level was increased (P<0.05) and HSP 90 mRNA (P<0.05) was decreased. In the liver, oxidation of protein was alleviated during acute heat challenge (P<0.05), however, gene expression HSF2, 3 and 4 and HSP 70 were highly induced (P<0.05). HSP90 expression was increased by chronic thermal treatment (P<0.05). In the muscle, both types of heat stress increased protein oxidation, but HSFs and HSPs gene expression remained unaltered. Only tendencies to increase were observed in HSP 70 (P = 0.052) and 90 (P = 0.054) gene expression after acute heat stress. The differential expressions of HSF and HSP genes in different tissues of laying broiler breeder chickens suggested that anti-heat stress mechanisms might be provoked more profoundly in the heart, by which the muscle was least protected during heat stress. In addition to HSP, HSFs gene expression could be used as a marker during acute heat stress.

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Rectal temperatures of laying broiler breeder chickens in response to the chronic heat challenge at 32°C over 8 weeks.In the first week, the rectal temperature of laying broiler breeder chickens in the chronic heat-stressed group (CS) was not elevated by the cyclic environmental temperature (32-35-32°C) and only a tendency of increase in rectal temperature was observed in the second week. After exposed to the consistent 32°C thermal treatment from the third week, CS birds had a significantly higher average body temperature than those in the chronic control group (CC). Values are expressed as means ± SE of data from 10–12 individual birds. * indicates significant differences (P<0.05) between CS (line with squares) and CC (line with diamonds) at the same time of the trial.
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pone-0102204-g002: Rectal temperatures of laying broiler breeder chickens in response to the chronic heat challenge at 32°C over 8 weeks.In the first week, the rectal temperature of laying broiler breeder chickens in the chronic heat-stressed group (CS) was not elevated by the cyclic environmental temperature (32-35-32°C) and only a tendency of increase in rectal temperature was observed in the second week. After exposed to the consistent 32°C thermal treatment from the third week, CS birds had a significantly higher average body temperature than those in the chronic control group (CC). Values are expressed as means ± SE of data from 10–12 individual birds. * indicates significant differences (P<0.05) between CS (line with squares) and CC (line with diamonds) at the same time of the trial.

Mentions: During chronic heat stress (experiment 2; Figure 2), the rectal temperature of birds in CS was not elevated by the cyclic environmental temperature in the first week and only a tendency of increment in rectal temperature was observed in the second week. After exposure to 32°C consistently from the third week on, the CS birds had significantly increased (P<0.05) average rectal temperature of 41.8°C±0.1 compared to 40.9°C±0.1 of control birds. Over the 8-week thermal challenge, heat stressed females had decreased (P<0.05) average feed intake (151.5±0.3 g/d) compared to control birds (162±0.3 g/d) and a reduction (P = 0.06) in average egg production (66.9±4.9%) compared to control birds (81.8±4.9%) during the 8-week experiment.


Differential expression of heat shock transcription factors and heat shock proteins after acute and chronic heat stress in laying chickens (Gallus gallus).

Xie J, Tang L, Lu L, Zhang L, Xi L, Liu HC, Odle J, Luo X - PLoS ONE (2014)

Rectal temperatures of laying broiler breeder chickens in response to the chronic heat challenge at 32°C over 8 weeks.In the first week, the rectal temperature of laying broiler breeder chickens in the chronic heat-stressed group (CS) was not elevated by the cyclic environmental temperature (32-35-32°C) and only a tendency of increase in rectal temperature was observed in the second week. After exposed to the consistent 32°C thermal treatment from the third week, CS birds had a significantly higher average body temperature than those in the chronic control group (CC). Values are expressed as means ± SE of data from 10–12 individual birds. * indicates significant differences (P<0.05) between CS (line with squares) and CC (line with diamonds) at the same time of the trial.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4114549&req=5

pone-0102204-g002: Rectal temperatures of laying broiler breeder chickens in response to the chronic heat challenge at 32°C over 8 weeks.In the first week, the rectal temperature of laying broiler breeder chickens in the chronic heat-stressed group (CS) was not elevated by the cyclic environmental temperature (32-35-32°C) and only a tendency of increase in rectal temperature was observed in the second week. After exposed to the consistent 32°C thermal treatment from the third week, CS birds had a significantly higher average body temperature than those in the chronic control group (CC). Values are expressed as means ± SE of data from 10–12 individual birds. * indicates significant differences (P<0.05) between CS (line with squares) and CC (line with diamonds) at the same time of the trial.
Mentions: During chronic heat stress (experiment 2; Figure 2), the rectal temperature of birds in CS was not elevated by the cyclic environmental temperature in the first week and only a tendency of increment in rectal temperature was observed in the second week. After exposure to 32°C consistently from the third week on, the CS birds had significantly increased (P<0.05) average rectal temperature of 41.8°C±0.1 compared to 40.9°C±0.1 of control birds. Over the 8-week thermal challenge, heat stressed females had decreased (P<0.05) average feed intake (151.5±0.3 g/d) compared to control birds (162±0.3 g/d) and a reduction (P = 0.06) in average egg production (66.9±4.9%) compared to control birds (81.8±4.9%) during the 8-week experiment.

Bottom Line: Tissue-specific responses to thermal challenge were also found among heart, liver and muscle.HSP90 expression was increased by chronic thermal treatment (P<0.05).The differential expressions of HSF and HSP genes in different tissues of laying broiler breeder chickens suggested that anti-heat stress mechanisms might be provoked more profoundly in the heart, by which the muscle was least protected during heat stress.

View Article: PubMed Central - PubMed

Affiliation: Mineral Nutrition Research Division, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, People's Republic of China.

ABSTRACT
Heat stress due to high environmental temperature negatively influences animal performances. To better understand the biological impact of heat stress, laying broiler breeder chickens were subjected either to acute (step-wisely increasing temperature from 21 to 35°C within 24 hours) or chronic (32°C for 8 weeks) high temperature exposure. High temperature challenges significantly elevated body temperature of experimental birds (P<0.05). However, oxidation status of lipid and protein and expression of heat shock transcription factors (HSFs) and heat shock proteins (HSPs) 70 and 90 were differently affected by acute and chronic treatment. Tissue-specific responses to thermal challenge were also found among heart, liver and muscle. In the heart, acute heat challenge affected lipid oxidation (P = 0.05) and gene expression of all 4 HSF gene expression was upregulated (P<0.05). During chronic heat treatment, the HSP 70 mRNA level was increased (P<0.05) and HSP 90 mRNA (P<0.05) was decreased. In the liver, oxidation of protein was alleviated during acute heat challenge (P<0.05), however, gene expression HSF2, 3 and 4 and HSP 70 were highly induced (P<0.05). HSP90 expression was increased by chronic thermal treatment (P<0.05). In the muscle, both types of heat stress increased protein oxidation, but HSFs and HSPs gene expression remained unaltered. Only tendencies to increase were observed in HSP 70 (P = 0.052) and 90 (P = 0.054) gene expression after acute heat stress. The differential expressions of HSF and HSP genes in different tissues of laying broiler breeder chickens suggested that anti-heat stress mechanisms might be provoked more profoundly in the heart, by which the muscle was least protected during heat stress. In addition to HSP, HSFs gene expression could be used as a marker during acute heat stress.

Show MeSH
Related in: MedlinePlus