Limits...
Infection of swine ex vivo tissues with avian viruses including H7N9 and correlation with glycomic analysis.

Chan RW, Karamanska R, Van Poucke S, Van Reeth K, Chan IW, Chan MC, Dell A, Peiris JS, Haslam SM, Guan Y, Nicholls JM - Influenza Other Respir Viruses (2013)

Bottom Line: There was a greater abundance of Gal-α-Gal linkages resulting in a relative decrease in sialylated glycans.The swine respiratory tract also had a greater proportion of glycans containing Neu5Gc and Siaα2-6 glycans than the human respiratory tract.In contrast to previous studies we found that there was not as much expression of Siaα2-3 glycans on the surface of the trachea.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR, China; Centre of Influenza Research, School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR, China.

Show MeSH

Related in: MedlinePlus

N‐glycan profile of mini‐pig lung. MALDI‐TOF mass spectra of permethylated N‐glycans from the intermedidate lobe of a mini‐pig lung was obtained from the 50% MeCN fraction from a C18 Sep‐Pak column (“Materials and Methods”). Annotated structures are according to the Consortium for Functional Glycomics guidelines. All molecular ions are [M+Na]+ Putative structures are based on composition, tandem MS, and the biosynthetic knowledge. Structures that show sugars outside a bracket have not been unequivocally defined. Sialylated species are annotated in red and those with GalαGal are annotated in blue.
© Copyright Policy - creativeCommonsBy-nc
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4114536&req=5

irv12144-fig-0006: N‐glycan profile of mini‐pig lung. MALDI‐TOF mass spectra of permethylated N‐glycans from the intermedidate lobe of a mini‐pig lung was obtained from the 50% MeCN fraction from a C18 Sep‐Pak column (“Materials and Methods”). Annotated structures are according to the Consortium for Functional Glycomics guidelines. All molecular ions are [M+Na]+ Putative structures are based on composition, tandem MS, and the biosynthetic knowledge. Structures that show sugars outside a bracket have not been unequivocally defined. Sialylated species are annotated in red and those with GalαGal are annotated in blue.

Mentions: We compared the two lungs from normal pigs with lung tissue obtained from a minipig (Figure 6). Differences between the normal and minipig lungs were identified. In particular, the minipig lung tissues had a high abundance of monosialylated and disialylated biantennary core fucosylated structures (NeuAcHex5HexNAc4Fuc, NeuAc2Hex5HexNAc4Fuc) at m/z 2605·3 and 2966·5, which was relatively low in the normal pig lung. In contrast, the biantennary glycan with the terminal Galα1‐3Gal m/z 2652·3 (Hex7HexNAc4Fuc) was more abundant in the normal pigs than tissues from minipigs. The NeuAc to NeuGc ratio was similar between the normal and minipigs in the biantennary glycans at m/z 2605·3, 2635·3, 2809·4, 2839·4, 3054·5, and 3084·5, but again this ratio appeared reversed for the larger tri‐ and tetra‐antennary structures. In addition, the data from sialidase treatment indicated a greater abundance of α2‐3‐type linkages in the minipig lung compared with the normal pig lung.


Infection of swine ex vivo tissues with avian viruses including H7N9 and correlation with glycomic analysis.

Chan RW, Karamanska R, Van Poucke S, Van Reeth K, Chan IW, Chan MC, Dell A, Peiris JS, Haslam SM, Guan Y, Nicholls JM - Influenza Other Respir Viruses (2013)

N‐glycan profile of mini‐pig lung. MALDI‐TOF mass spectra of permethylated N‐glycans from the intermedidate lobe of a mini‐pig lung was obtained from the 50% MeCN fraction from a C18 Sep‐Pak column (“Materials and Methods”). Annotated structures are according to the Consortium for Functional Glycomics guidelines. All molecular ions are [M+Na]+ Putative structures are based on composition, tandem MS, and the biosynthetic knowledge. Structures that show sugars outside a bracket have not been unequivocally defined. Sialylated species are annotated in red and those with GalαGal are annotated in blue.
© Copyright Policy - creativeCommonsBy-nc
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4114536&req=5

irv12144-fig-0006: N‐glycan profile of mini‐pig lung. MALDI‐TOF mass spectra of permethylated N‐glycans from the intermedidate lobe of a mini‐pig lung was obtained from the 50% MeCN fraction from a C18 Sep‐Pak column (“Materials and Methods”). Annotated structures are according to the Consortium for Functional Glycomics guidelines. All molecular ions are [M+Na]+ Putative structures are based on composition, tandem MS, and the biosynthetic knowledge. Structures that show sugars outside a bracket have not been unequivocally defined. Sialylated species are annotated in red and those with GalαGal are annotated in blue.
Mentions: We compared the two lungs from normal pigs with lung tissue obtained from a minipig (Figure 6). Differences between the normal and minipig lungs were identified. In particular, the minipig lung tissues had a high abundance of monosialylated and disialylated biantennary core fucosylated structures (NeuAcHex5HexNAc4Fuc, NeuAc2Hex5HexNAc4Fuc) at m/z 2605·3 and 2966·5, which was relatively low in the normal pig lung. In contrast, the biantennary glycan with the terminal Galα1‐3Gal m/z 2652·3 (Hex7HexNAc4Fuc) was more abundant in the normal pigs than tissues from minipigs. The NeuAc to NeuGc ratio was similar between the normal and minipigs in the biantennary glycans at m/z 2605·3, 2635·3, 2809·4, 2839·4, 3054·5, and 3084·5, but again this ratio appeared reversed for the larger tri‐ and tetra‐antennary structures. In addition, the data from sialidase treatment indicated a greater abundance of α2‐3‐type linkages in the minipig lung compared with the normal pig lung.

Bottom Line: There was a greater abundance of Gal-α-Gal linkages resulting in a relative decrease in sialylated glycans.The swine respiratory tract also had a greater proportion of glycans containing Neu5Gc and Siaα2-6 glycans than the human respiratory tract.In contrast to previous studies we found that there was not as much expression of Siaα2-3 glycans on the surface of the trachea.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR, China; Centre of Influenza Research, School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR, China.

Show MeSH
Related in: MedlinePlus