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Iron deficiency alters megakaryopoiesis and platelet phenotype independent of thrombopoietin.

Evstatiev R, Bukaty A, Jimenez K, Kulnigg-Dabsch S, Surman L, Schmid W, Eferl R, Lippert K, Scheiber-Mojdehkar B, Kvasnicka HM, Khare V, Gasche C - Am. J. Hematol. (2014)

Bottom Line: Moreover, platelets produced in iron deficient animals displayed a higher mean platelet volume and increased aggregation.Iron deficiency did not alter the production of hematopoietic growth factors such as thrombopoietin, interleukin 6 or interleukin 11.Megakaryocytic cell lines grown in iron-depleted conditions exhibited reduced proliferation but increased ploidy and cell size.

View Article: PubMed Central - PubMed

Affiliation: Division of Gastroenterology and Hepatology, Department of Medicine III, Medical University of Vienna, Vienna, Austria; Christian Doppler Laboratory on Molecular Cancer Chemoprevention, Medical University of Vienna, Vienna, Austria.

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 Iron deficiency inhibits proliferation but increases ploidy in megakaryocytic cell lines. HEL, Dami and CMK cells were grown in serum‐free medium either repleted with iron (control) or without iron‐ and transferrin (ID). ID led to a significant decrease in proliferation as assessed by MTT assays (A) but to an increase in polyploid cells (>4n), measured by flow cytometry (B (representative flow cytometry histograms of Hoechst‐stained HEL cells); C). In parallel, cell size increased (demonstrated for HEL cells, D). The results from 2 to 3 independent experiments are shown.
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ajh23682-fig-0004:  Iron deficiency inhibits proliferation but increases ploidy in megakaryocytic cell lines. HEL, Dami and CMK cells were grown in serum‐free medium either repleted with iron (control) or without iron‐ and transferrin (ID). ID led to a significant decrease in proliferation as assessed by MTT assays (A) but to an increase in polyploid cells (>4n), measured by flow cytometry (B (representative flow cytometry histograms of Hoechst‐stained HEL cells); C). In parallel, cell size increased (demonstrated for HEL cells, D). The results from 2 to 3 independent experiments are shown.

Mentions: To test whether ID influences megakaryopiesis independently of hematopoietic growth factors, we examined the effect of ID growth conditions in the megakaryocytic cell lines Dami, HEL and CMK. ID inhibited proliferation in all cell lines, as reflected by the MTT assays (Fig. 4A). At the same time, the fraction of polyploid cells (i.e., > 4n) increased about two‐fold under ID (Fig. 4B,C). These changes in ploidy were paralleled by an increase in cell size, as observed by light microscopy (Fig. 4D).


Iron deficiency alters megakaryopoiesis and platelet phenotype independent of thrombopoietin.

Evstatiev R, Bukaty A, Jimenez K, Kulnigg-Dabsch S, Surman L, Schmid W, Eferl R, Lippert K, Scheiber-Mojdehkar B, Kvasnicka HM, Khare V, Gasche C - Am. J. Hematol. (2014)

 Iron deficiency inhibits proliferation but increases ploidy in megakaryocytic cell lines. HEL, Dami and CMK cells were grown in serum‐free medium either repleted with iron (control) or without iron‐ and transferrin (ID). ID led to a significant decrease in proliferation as assessed by MTT assays (A) but to an increase in polyploid cells (>4n), measured by flow cytometry (B (representative flow cytometry histograms of Hoechst‐stained HEL cells); C). In parallel, cell size increased (demonstrated for HEL cells, D). The results from 2 to 3 independent experiments are shown.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4114532&req=5

ajh23682-fig-0004:  Iron deficiency inhibits proliferation but increases ploidy in megakaryocytic cell lines. HEL, Dami and CMK cells were grown in serum‐free medium either repleted with iron (control) or without iron‐ and transferrin (ID). ID led to a significant decrease in proliferation as assessed by MTT assays (A) but to an increase in polyploid cells (>4n), measured by flow cytometry (B (representative flow cytometry histograms of Hoechst‐stained HEL cells); C). In parallel, cell size increased (demonstrated for HEL cells, D). The results from 2 to 3 independent experiments are shown.
Mentions: To test whether ID influences megakaryopiesis independently of hematopoietic growth factors, we examined the effect of ID growth conditions in the megakaryocytic cell lines Dami, HEL and CMK. ID inhibited proliferation in all cell lines, as reflected by the MTT assays (Fig. 4A). At the same time, the fraction of polyploid cells (i.e., > 4n) increased about two‐fold under ID (Fig. 4B,C). These changes in ploidy were paralleled by an increase in cell size, as observed by light microscopy (Fig. 4D).

Bottom Line: Moreover, platelets produced in iron deficient animals displayed a higher mean platelet volume and increased aggregation.Iron deficiency did not alter the production of hematopoietic growth factors such as thrombopoietin, interleukin 6 or interleukin 11.Megakaryocytic cell lines grown in iron-depleted conditions exhibited reduced proliferation but increased ploidy and cell size.

View Article: PubMed Central - PubMed

Affiliation: Division of Gastroenterology and Hepatology, Department of Medicine III, Medical University of Vienna, Vienna, Austria; Christian Doppler Laboratory on Molecular Cancer Chemoprevention, Medical University of Vienna, Vienna, Austria.

Show MeSH
Related in: MedlinePlus