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Electroacupuncture enhances extragonadal aromatization in ovariectomized rats.

Zhao H, Tian Z, Cheng L, Chen B - Reprod. Biol. Endocrinol. (2004)

Bottom Line: To explore the source of the increased circulation estrogen, the extragonadal aromatization was detected.The blood concentrations of estrogen, testosterone and corticosterone were measured by radioimmunoassay.Both the subcutaneous abdominal adipose and the liver tissues contributed to the effects of electroacupuncture on the extragonadal aromatization to promote the blood concentrations of estrogen in the ovariectomized rats.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Neurobiology and Integrative Medicine, Institute of Acupuncture Research , WHO Collaborating Center for Traditional Medicine, Shanghai Medical College of Fudan University, Shanghai, P R China. zh2000h@hotmail.com

ABSTRACT

Background: Repeated electroacupuncture (EA) stimulation is known to stimulate the activity of the hypothalamus-pituitary-adrenal axis, and to enhance the circulation level of estrogen in the ovariectomized rats. To explore the source of the increased circulation estrogen, the extragonadal aromatization was detected.

Methods: Female rats were divided into five groups: 1) intact (INT), 2) intact with EA in specific points (INT+EA), 3) ovariectomized (OVX), 4) ovariectomized with EA in specific points (OVX+EA) and 5) ovariectomized with EA in non-specific points (OVX+C). Radiometric assay, Western blot and RT-PCR were adopted to determine the extragonadal aromatization in subcutaneous abdominal (SA) adipose and liver tissues of rats. The blood concentrations of estrogen, testosterone and corticosterone were measured by radioimmunoassay.

Results: The aromatase activities of the SA adipose and liver tissues in the OVX+EA rats increased significantly (p < 0.01) compared with those in the INT, INT+EA and OVX rats. The 58-kDa aromatase protein and aromatase mRNA expressions normalized to beta-actin in the OVX+EA rats' SA adipose tissues showed higher levels than those from corresponding tissues in the INT and INT+EA rats (p < 0.05). And the ratios of aromatase mRNA and protein to beta-actin in the OVX+EA rats' liver tissues increased significantly compared with those in the OVX rats (p < 0.05). Furthermore, blood estrogen and corticosterone concentrations showed significant increase in the OVX+EA rats compared with the concentrations in the OVX and OVX+C rats (p < 0.05), but no statistical disparity occurred on the blood testosterone concentrations between the OVX+EA rats and the OVX ones.

Conclusion: Both the subcutaneous abdominal adipose and the liver tissues contributed to the effects of electroacupuncture on the extragonadal aromatization to promote the blood concentrations of estrogen in the ovariectomized rats.

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Effects of the electroacupuncture on the aromatase mRNA expressions by RT-PCR analysis. The upper picture shows the gel electrophoresis of the RT-PCR products for the aromatase. Total RNA fractions were isolated from the SA adipose and liver tissues of the INT, INT+EA, OVX and OVX+EA rats, and the cDNAs were prepared using standard techniques, as described in materials and methods. The RT-PCR products for aromatase were fractionated by electrophoresis through 2% agarose gels. Densitometric analysis of the mRNA concentration using aromatase/β-actin expressed as the mean with SEM bar (nINT = 12, nINT + EA = 12, nOVX = 12 and nOVX + EA = 10) in each column indicated in the lower panel. * p < 0.05 vs INT and INT+EA, # p < 0.05 vs OVX.
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Figure 5: Effects of the electroacupuncture on the aromatase mRNA expressions by RT-PCR analysis. The upper picture shows the gel electrophoresis of the RT-PCR products for the aromatase. Total RNA fractions were isolated from the SA adipose and liver tissues of the INT, INT+EA, OVX and OVX+EA rats, and the cDNAs were prepared using standard techniques, as described in materials and methods. The RT-PCR products for aromatase were fractionated by electrophoresis through 2% agarose gels. Densitometric analysis of the mRNA concentration using aromatase/β-actin expressed as the mean with SEM bar (nINT = 12, nINT + EA = 12, nOVX = 12 and nOVX + EA = 10) in each column indicated in the lower panel. * p < 0.05 vs INT and INT+EA, # p < 0.05 vs OVX.

Mentions: Comparison of the amplified PCR fragment with rat ovary aromatase sequence revealed 100% homology (data not shown). Densitometric analysis of the mRNA concentration using aromatase/β-actin expressed as the mean with SEM. The ratio of liver aromatase to β-actin in the OVX decreased significantly compared with that in the INT (p < 0.05), and the ratio of liver tissue in the OVX+EA was higher than in the OVX (p < 0.05). No changes occurred on the ratios of SA adipose tissue between the OVX and INT, but a significant increase of the ratio in the OVX+EA (p < 0.05) compared with that in the INT and OVX was detected. However, no disparity was found between the INT and INT+EA (Fig. 5).


Electroacupuncture enhances extragonadal aromatization in ovariectomized rats.

Zhao H, Tian Z, Cheng L, Chen B - Reprod. Biol. Endocrinol. (2004)

Effects of the electroacupuncture on the aromatase mRNA expressions by RT-PCR analysis. The upper picture shows the gel electrophoresis of the RT-PCR products for the aromatase. Total RNA fractions were isolated from the SA adipose and liver tissues of the INT, INT+EA, OVX and OVX+EA rats, and the cDNAs were prepared using standard techniques, as described in materials and methods. The RT-PCR products for aromatase were fractionated by electrophoresis through 2% agarose gels. Densitometric analysis of the mRNA concentration using aromatase/β-actin expressed as the mean with SEM bar (nINT = 12, nINT + EA = 12, nOVX = 12 and nOVX + EA = 10) in each column indicated in the lower panel. * p < 0.05 vs INT and INT+EA, # p < 0.05 vs OVX.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC411058&req=5

Figure 5: Effects of the electroacupuncture on the aromatase mRNA expressions by RT-PCR analysis. The upper picture shows the gel electrophoresis of the RT-PCR products for the aromatase. Total RNA fractions were isolated from the SA adipose and liver tissues of the INT, INT+EA, OVX and OVX+EA rats, and the cDNAs were prepared using standard techniques, as described in materials and methods. The RT-PCR products for aromatase were fractionated by electrophoresis through 2% agarose gels. Densitometric analysis of the mRNA concentration using aromatase/β-actin expressed as the mean with SEM bar (nINT = 12, nINT + EA = 12, nOVX = 12 and nOVX + EA = 10) in each column indicated in the lower panel. * p < 0.05 vs INT and INT+EA, # p < 0.05 vs OVX.
Mentions: Comparison of the amplified PCR fragment with rat ovary aromatase sequence revealed 100% homology (data not shown). Densitometric analysis of the mRNA concentration using aromatase/β-actin expressed as the mean with SEM. The ratio of liver aromatase to β-actin in the OVX decreased significantly compared with that in the INT (p < 0.05), and the ratio of liver tissue in the OVX+EA was higher than in the OVX (p < 0.05). No changes occurred on the ratios of SA adipose tissue between the OVX and INT, but a significant increase of the ratio in the OVX+EA (p < 0.05) compared with that in the INT and OVX was detected. However, no disparity was found between the INT and INT+EA (Fig. 5).

Bottom Line: To explore the source of the increased circulation estrogen, the extragonadal aromatization was detected.The blood concentrations of estrogen, testosterone and corticosterone were measured by radioimmunoassay.Both the subcutaneous abdominal adipose and the liver tissues contributed to the effects of electroacupuncture on the extragonadal aromatization to promote the blood concentrations of estrogen in the ovariectomized rats.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Neurobiology and Integrative Medicine, Institute of Acupuncture Research , WHO Collaborating Center for Traditional Medicine, Shanghai Medical College of Fudan University, Shanghai, P R China. zh2000h@hotmail.com

ABSTRACT

Background: Repeated electroacupuncture (EA) stimulation is known to stimulate the activity of the hypothalamus-pituitary-adrenal axis, and to enhance the circulation level of estrogen in the ovariectomized rats. To explore the source of the increased circulation estrogen, the extragonadal aromatization was detected.

Methods: Female rats were divided into five groups: 1) intact (INT), 2) intact with EA in specific points (INT+EA), 3) ovariectomized (OVX), 4) ovariectomized with EA in specific points (OVX+EA) and 5) ovariectomized with EA in non-specific points (OVX+C). Radiometric assay, Western blot and RT-PCR were adopted to determine the extragonadal aromatization in subcutaneous abdominal (SA) adipose and liver tissues of rats. The blood concentrations of estrogen, testosterone and corticosterone were measured by radioimmunoassay.

Results: The aromatase activities of the SA adipose and liver tissues in the OVX+EA rats increased significantly (p < 0.01) compared with those in the INT, INT+EA and OVX rats. The 58-kDa aromatase protein and aromatase mRNA expressions normalized to beta-actin in the OVX+EA rats' SA adipose tissues showed higher levels than those from corresponding tissues in the INT and INT+EA rats (p < 0.05). And the ratios of aromatase mRNA and protein to beta-actin in the OVX+EA rats' liver tissues increased significantly compared with those in the OVX rats (p < 0.05). Furthermore, blood estrogen and corticosterone concentrations showed significant increase in the OVX+EA rats compared with the concentrations in the OVX and OVX+C rats (p < 0.05), but no statistical disparity occurred on the blood testosterone concentrations between the OVX+EA rats and the OVX ones.

Conclusion: Both the subcutaneous abdominal adipose and the liver tissues contributed to the effects of electroacupuncture on the extragonadal aromatization to promote the blood concentrations of estrogen in the ovariectomized rats.

Show MeSH