Limits...
Electroacupuncture enhances extragonadal aromatization in ovariectomized rats.

Zhao H, Tian Z, Cheng L, Chen B - Reprod. Biol. Endocrinol. (2004)

Bottom Line: To explore the source of the increased circulation estrogen, the extragonadal aromatization was detected.The blood concentrations of estrogen, testosterone and corticosterone were measured by radioimmunoassay.Both the subcutaneous abdominal adipose and the liver tissues contributed to the effects of electroacupuncture on the extragonadal aromatization to promote the blood concentrations of estrogen in the ovariectomized rats.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Neurobiology and Integrative Medicine, Institute of Acupuncture Research , WHO Collaborating Center for Traditional Medicine, Shanghai Medical College of Fudan University, Shanghai, P R China. zh2000h@hotmail.com

ABSTRACT

Background: Repeated electroacupuncture (EA) stimulation is known to stimulate the activity of the hypothalamus-pituitary-adrenal axis, and to enhance the circulation level of estrogen in the ovariectomized rats. To explore the source of the increased circulation estrogen, the extragonadal aromatization was detected.

Methods: Female rats were divided into five groups: 1) intact (INT), 2) intact with EA in specific points (INT+EA), 3) ovariectomized (OVX), 4) ovariectomized with EA in specific points (OVX+EA) and 5) ovariectomized with EA in non-specific points (OVX+C). Radiometric assay, Western blot and RT-PCR were adopted to determine the extragonadal aromatization in subcutaneous abdominal (SA) adipose and liver tissues of rats. The blood concentrations of estrogen, testosterone and corticosterone were measured by radioimmunoassay.

Results: The aromatase activities of the SA adipose and liver tissues in the OVX+EA rats increased significantly (p < 0.01) compared with those in the INT, INT+EA and OVX rats. The 58-kDa aromatase protein and aromatase mRNA expressions normalized to beta-actin in the OVX+EA rats' SA adipose tissues showed higher levels than those from corresponding tissues in the INT and INT+EA rats (p < 0.05). And the ratios of aromatase mRNA and protein to beta-actin in the OVX+EA rats' liver tissues increased significantly compared with those in the OVX rats (p < 0.05). Furthermore, blood estrogen and corticosterone concentrations showed significant increase in the OVX+EA rats compared with the concentrations in the OVX and OVX+C rats (p < 0.05), but no statistical disparity occurred on the blood testosterone concentrations between the OVX+EA rats and the OVX ones.

Conclusion: Both the subcutaneous abdominal adipose and the liver tissues contributed to the effects of electroacupuncture on the extragonadal aromatization to promote the blood concentrations of estrogen in the ovariectomized rats.

Show MeSH
Sketch of ventral view (A) and dorsal view (B) of rat shows the acupoints we used.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC411058&req=5

Figure 1: Sketch of ventral view (A) and dorsal view (B) of rat shows the acupoints we used.

Mentions: Sixty female Sprague-Dawley rats (180–200 g), with regular 4-day estrus cycles were purchased from Medical Experimental Animals Center of Fudan University (Shanghai, China). The animals were housed under laminar flow in an isolated room with controlled temperature and at a 12 /12 (light /dark) schedule. Thirty-six of them underwent ovariectomy with ether anaesthesia, which were then divided randomly into three groups: ovariectomized (OVX), ovariectomized with EA in specific points (OVX+EA) and ovariectomized with EA in non-specific points (OVX+C). The rest were treated as controls, which were divided into two groups: intact (INT) and intact with EA in specific points (INT+EA). Four weeks later, OVX+EA (n = 12), OVX+C (n = 12) and INT+EA (n = 12) received EA treatment. Thirty minutes before the EA treatment, all the animals were bound as comfortably as possible, and during the EA procedure, the rats were conscious without anaesthesia. Electrical stimulation was administered via three stainless steel needles of 0.3 mm diameter inserted 5 mm in four acupoints in the belly: "Guanyuan" acupoint (RN4), in the midline of abdomen (15 mm bellow the umbilicus); "Zhongji" acupoint (RN3), 5 mm bellow "Guanyuan" acupoints (one needle was flatly punctured in the RN4 which penetrated into the RN3); bilateral "Zigongxue" acupoints (EXTRA22), 7.5 mm lateral to the "Zhongji" acupoint, and one needle inserted 3 mm at one acupoint in the hind leg, "Sanyinjiao" (SP6), near ankle joint (at the concentration of the superior border of the medial melleolus, between the posterior border of the tibia and anterior border of the Achilles tendon). These acupoints are widely applied in Oriental medicine for the treatment of gynaecological disease in women [8] and for the acupuncture mechanism research in the ovariectomized rats [4,5,7,9]. The control acupoints were "Waiguan" (SJ5), between the radius and ulna (5 mm above the dorsal transverse crease of the wrist) and "Huatuojiaji" (EXTRA15), in the back (5 mm lateral to the lower border of each spinous process from the first thoracic vertebra to the fifth lumbar vertebra (Fig. 1). The stimulation was generated by an EA apparatus (Model G6805-2, SMIF, Shanghai, China) and lasted for 30 min (8:00–10:00 AM), Q.D, for 3 days altogether. The stimulation parameters were 2 mA of density and a low-burst frequency of 3 Hz. Individual pulses within the burst frequency were square wave pulses with alternating polarities and pulse duration of 0.2 ms, 80 pulse per second. The intensity was adjusted to produce a slight twitch of the limbs. All experimental procedures involving the use of animals were conducted in accordance with NIH Guidelines and were reviewed and approved by the Animal Use and Care Committee for the Fudan University.


Electroacupuncture enhances extragonadal aromatization in ovariectomized rats.

Zhao H, Tian Z, Cheng L, Chen B - Reprod. Biol. Endocrinol. (2004)

Sketch of ventral view (A) and dorsal view (B) of rat shows the acupoints we used.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC411058&req=5

Figure 1: Sketch of ventral view (A) and dorsal view (B) of rat shows the acupoints we used.
Mentions: Sixty female Sprague-Dawley rats (180–200 g), with regular 4-day estrus cycles were purchased from Medical Experimental Animals Center of Fudan University (Shanghai, China). The animals were housed under laminar flow in an isolated room with controlled temperature and at a 12 /12 (light /dark) schedule. Thirty-six of them underwent ovariectomy with ether anaesthesia, which were then divided randomly into three groups: ovariectomized (OVX), ovariectomized with EA in specific points (OVX+EA) and ovariectomized with EA in non-specific points (OVX+C). The rest were treated as controls, which were divided into two groups: intact (INT) and intact with EA in specific points (INT+EA). Four weeks later, OVX+EA (n = 12), OVX+C (n = 12) and INT+EA (n = 12) received EA treatment. Thirty minutes before the EA treatment, all the animals were bound as comfortably as possible, and during the EA procedure, the rats were conscious without anaesthesia. Electrical stimulation was administered via three stainless steel needles of 0.3 mm diameter inserted 5 mm in four acupoints in the belly: "Guanyuan" acupoint (RN4), in the midline of abdomen (15 mm bellow the umbilicus); "Zhongji" acupoint (RN3), 5 mm bellow "Guanyuan" acupoints (one needle was flatly punctured in the RN4 which penetrated into the RN3); bilateral "Zigongxue" acupoints (EXTRA22), 7.5 mm lateral to the "Zhongji" acupoint, and one needle inserted 3 mm at one acupoint in the hind leg, "Sanyinjiao" (SP6), near ankle joint (at the concentration of the superior border of the medial melleolus, between the posterior border of the tibia and anterior border of the Achilles tendon). These acupoints are widely applied in Oriental medicine for the treatment of gynaecological disease in women [8] and for the acupuncture mechanism research in the ovariectomized rats [4,5,7,9]. The control acupoints were "Waiguan" (SJ5), between the radius and ulna (5 mm above the dorsal transverse crease of the wrist) and "Huatuojiaji" (EXTRA15), in the back (5 mm lateral to the lower border of each spinous process from the first thoracic vertebra to the fifth lumbar vertebra (Fig. 1). The stimulation was generated by an EA apparatus (Model G6805-2, SMIF, Shanghai, China) and lasted for 30 min (8:00–10:00 AM), Q.D, for 3 days altogether. The stimulation parameters were 2 mA of density and a low-burst frequency of 3 Hz. Individual pulses within the burst frequency were square wave pulses with alternating polarities and pulse duration of 0.2 ms, 80 pulse per second. The intensity was adjusted to produce a slight twitch of the limbs. All experimental procedures involving the use of animals were conducted in accordance with NIH Guidelines and were reviewed and approved by the Animal Use and Care Committee for the Fudan University.

Bottom Line: To explore the source of the increased circulation estrogen, the extragonadal aromatization was detected.The blood concentrations of estrogen, testosterone and corticosterone were measured by radioimmunoassay.Both the subcutaneous abdominal adipose and the liver tissues contributed to the effects of electroacupuncture on the extragonadal aromatization to promote the blood concentrations of estrogen in the ovariectomized rats.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Neurobiology and Integrative Medicine, Institute of Acupuncture Research , WHO Collaborating Center for Traditional Medicine, Shanghai Medical College of Fudan University, Shanghai, P R China. zh2000h@hotmail.com

ABSTRACT

Background: Repeated electroacupuncture (EA) stimulation is known to stimulate the activity of the hypothalamus-pituitary-adrenal axis, and to enhance the circulation level of estrogen in the ovariectomized rats. To explore the source of the increased circulation estrogen, the extragonadal aromatization was detected.

Methods: Female rats were divided into five groups: 1) intact (INT), 2) intact with EA in specific points (INT+EA), 3) ovariectomized (OVX), 4) ovariectomized with EA in specific points (OVX+EA) and 5) ovariectomized with EA in non-specific points (OVX+C). Radiometric assay, Western blot and RT-PCR were adopted to determine the extragonadal aromatization in subcutaneous abdominal (SA) adipose and liver tissues of rats. The blood concentrations of estrogen, testosterone and corticosterone were measured by radioimmunoassay.

Results: The aromatase activities of the SA adipose and liver tissues in the OVX+EA rats increased significantly (p < 0.01) compared with those in the INT, INT+EA and OVX rats. The 58-kDa aromatase protein and aromatase mRNA expressions normalized to beta-actin in the OVX+EA rats' SA adipose tissues showed higher levels than those from corresponding tissues in the INT and INT+EA rats (p < 0.05). And the ratios of aromatase mRNA and protein to beta-actin in the OVX+EA rats' liver tissues increased significantly compared with those in the OVX rats (p < 0.05). Furthermore, blood estrogen and corticosterone concentrations showed significant increase in the OVX+EA rats compared with the concentrations in the OVX and OVX+C rats (p < 0.05), but no statistical disparity occurred on the blood testosterone concentrations between the OVX+EA rats and the OVX ones.

Conclusion: Both the subcutaneous abdominal adipose and the liver tissues contributed to the effects of electroacupuncture on the extragonadal aromatization to promote the blood concentrations of estrogen in the ovariectomized rats.

Show MeSH