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Microfluidics in biotechnology.

Barry R, Ivanov D - J Nanobiotechnology (2004)

Bottom Line: Microfluidics enables biotechnological processes to proceed on a scale (microns) at which physical processes such as osmotic movement, electrophoretic-motility and surface interactions become enhanced.The versatility of microfluidic devices allows interfacing with current methods and technologies.The flexibility of microfluidics will facilitate its exploitation in assay development across multiple biotechnological disciplines.

View Article: PubMed Central - HTML - PubMed

Affiliation: School of Biological Sciences Royal Holloway, University of London Egham, Surrey TW20 0EX United Kingdom. Richard.Barry@rhul.ac.uk

ABSTRACT
Microfluidics enables biotechnological processes to proceed on a scale (microns) at which physical processes such as osmotic movement, electrophoretic-motility and surface interactions become enhanced. At the microscale sample volumes and assay times are reduced, and procedural costs are lowered. The versatility of microfluidic devices allows interfacing with current methods and technologies. Microfluidics has been applied to DNA analysis methods and shown to accelerate DNA microarray assay hybridisation times. The linking of microfluidics to protein analysis techologies, e.g. mass spectrometry, enables picomole amounts of peptide to be analysed within a controlled micro-environment. The flexibility of microfluidics will facilitate its exploitation in assay development across multiple biotechnological disciplines.

No MeSH data available.


Microfluidic mass spectrometric protein analysis. Proteins are applied directly to a membrane, desalted and directed by microfluidic channel to mass spectrometric analysis.
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Figure 2: Microfluidic mass spectrometric protein analysis. Proteins are applied directly to a membrane, desalted and directed by microfluidic channel to mass spectrometric analysis.

Mentions: Microfluidic technology has also been incorporated into the analysis of proteins / peptides [7,8]. In particular, microfluidics can be linked with a mass spectrometric analysis of proteins or peptides. Thus, peptides can be adsorbed onto hydrophobic membranes, desalted, and through the use of microfluidics eluted in a controlled manner to allow the direct mass spectrometric analysis of picomole amounts of peptides by electrospray ionisation mass spectrometry procedures [9], Figure 2.


Microfluidics in biotechnology.

Barry R, Ivanov D - J Nanobiotechnology (2004)

Microfluidic mass spectrometric protein analysis. Proteins are applied directly to a membrane, desalted and directed by microfluidic channel to mass spectrometric analysis.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC411055&req=5

Figure 2: Microfluidic mass spectrometric protein analysis. Proteins are applied directly to a membrane, desalted and directed by microfluidic channel to mass spectrometric analysis.
Mentions: Microfluidic technology has also been incorporated into the analysis of proteins / peptides [7,8]. In particular, microfluidics can be linked with a mass spectrometric analysis of proteins or peptides. Thus, peptides can be adsorbed onto hydrophobic membranes, desalted, and through the use of microfluidics eluted in a controlled manner to allow the direct mass spectrometric analysis of picomole amounts of peptides by electrospray ionisation mass spectrometry procedures [9], Figure 2.

Bottom Line: Microfluidics enables biotechnological processes to proceed on a scale (microns) at which physical processes such as osmotic movement, electrophoretic-motility and surface interactions become enhanced.The versatility of microfluidic devices allows interfacing with current methods and technologies.The flexibility of microfluidics will facilitate its exploitation in assay development across multiple biotechnological disciplines.

View Article: PubMed Central - HTML - PubMed

Affiliation: School of Biological Sciences Royal Holloway, University of London Egham, Surrey TW20 0EX United Kingdom. Richard.Barry@rhul.ac.uk

ABSTRACT
Microfluidics enables biotechnological processes to proceed on a scale (microns) at which physical processes such as osmotic movement, electrophoretic-motility and surface interactions become enhanced. At the microscale sample volumes and assay times are reduced, and procedural costs are lowered. The versatility of microfluidic devices allows interfacing with current methods and technologies. Microfluidics has been applied to DNA analysis methods and shown to accelerate DNA microarray assay hybridisation times. The linking of microfluidics to protein analysis techologies, e.g. mass spectrometry, enables picomole amounts of peptide to be analysed within a controlled micro-environment. The flexibility of microfluidics will facilitate its exploitation in assay development across multiple biotechnological disciplines.

No MeSH data available.