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Microfluidics in biotechnology.

Barry R, Ivanov D - J Nanobiotechnology (2004)

Bottom Line: Microfluidics enables biotechnological processes to proceed on a scale (microns) at which physical processes such as osmotic movement, electrophoretic-motility and surface interactions become enhanced.The versatility of microfluidic devices allows interfacing with current methods and technologies.The flexibility of microfluidics will facilitate its exploitation in assay development across multiple biotechnological disciplines.

View Article: PubMed Central - HTML - PubMed

Affiliation: School of Biological Sciences Royal Holloway, University of London Egham, Surrey TW20 0EX United Kingdom. Richard.Barry@rhul.ac.uk

ABSTRACT
Microfluidics enables biotechnological processes to proceed on a scale (microns) at which physical processes such as osmotic movement, electrophoretic-motility and surface interactions become enhanced. At the microscale sample volumes and assay times are reduced, and procedural costs are lowered. The versatility of microfluidic devices allows interfacing with current methods and technologies. Microfluidics has been applied to DNA analysis methods and shown to accelerate DNA microarray assay hybridisation times. The linking of microfluidics to protein analysis techologies, e.g. mass spectrometry, enables picomole amounts of peptide to be analysed within a controlled micro-environment. The flexibility of microfluidics will facilitate its exploitation in assay development across multiple biotechnological disciplines.

No MeSH data available.


Capillary flow direct PCR analysis. Whole blood samples are used for direct PCR analysis. Samples are manipulated within microfluidic channels.
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Figure 1: Capillary flow direct PCR analysis. Whole blood samples are used for direct PCR analysis. Samples are manipulated within microfluidic channels.

Mentions: Some specific microfluidic systems have been developed that are capable of a range of DNA-type analyses. A microfluidic integrated system, which minimises sample processing and handling, has been developed for PCR analysis. Here DNA typing is achieved from whole blood samples using capillary microfluidics and capillary array electrophoresis [1], see Figure 1, whereby blood is used directly as the sample template for a PCR amplification analysis.


Microfluidics in biotechnology.

Barry R, Ivanov D - J Nanobiotechnology (2004)

Capillary flow direct PCR analysis. Whole blood samples are used for direct PCR analysis. Samples are manipulated within microfluidic channels.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC411055&req=5

Figure 1: Capillary flow direct PCR analysis. Whole blood samples are used for direct PCR analysis. Samples are manipulated within microfluidic channels.
Mentions: Some specific microfluidic systems have been developed that are capable of a range of DNA-type analyses. A microfluidic integrated system, which minimises sample processing and handling, has been developed for PCR analysis. Here DNA typing is achieved from whole blood samples using capillary microfluidics and capillary array electrophoresis [1], see Figure 1, whereby blood is used directly as the sample template for a PCR amplification analysis.

Bottom Line: Microfluidics enables biotechnological processes to proceed on a scale (microns) at which physical processes such as osmotic movement, electrophoretic-motility and surface interactions become enhanced.The versatility of microfluidic devices allows interfacing with current methods and technologies.The flexibility of microfluidics will facilitate its exploitation in assay development across multiple biotechnological disciplines.

View Article: PubMed Central - HTML - PubMed

Affiliation: School of Biological Sciences Royal Holloway, University of London Egham, Surrey TW20 0EX United Kingdom. Richard.Barry@rhul.ac.uk

ABSTRACT
Microfluidics enables biotechnological processes to proceed on a scale (microns) at which physical processes such as osmotic movement, electrophoretic-motility and surface interactions become enhanced. At the microscale sample volumes and assay times are reduced, and procedural costs are lowered. The versatility of microfluidic devices allows interfacing with current methods and technologies. Microfluidics has been applied to DNA analysis methods and shown to accelerate DNA microarray assay hybridisation times. The linking of microfluidics to protein analysis techologies, e.g. mass spectrometry, enables picomole amounts of peptide to be analysed within a controlled micro-environment. The flexibility of microfluidics will facilitate its exploitation in assay development across multiple biotechnological disciplines.

No MeSH data available.