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Effect of Gynosaponin on Rumen In vitro Methanogenesis under Different Forage-Concentrate Ratios.

Manatbay B, Cheng Y, Mao S, Zhu W - Asian-australas. J. Anim. Sci. (2014)

Bottom Line: In the presence of gynosaponin, methane production and acetate concentration were significantly decreased, whereas concentration of propionate tended to be increased resulting in a significant reduction (p<0.05) of acetate:propionate ratio (A:P ratio), in high-forage substrate.However, gynosaponin treatment under high-concentrate level did not affect the methanogenesis, fermentation characteristics and tested microbes.Accordingly, overall results suggested that gynosaponin supplementation reduced the in vitro methanogenesis and improved rumen fermentation under high-forage condition by changing the abundances of related rumen microbes.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Gastrointestinal Microbiology, Nanjing Agricultural University, Nanjing 210095, China.

ABSTRACT
The study aimed to investigate the effects of gynosaponin on in vitro methanogenesis under different forage-concentrate ratios (F:C ratios). Experiment was conducted with two kinds of F:C ratios (F:C = 7:3 and F:C = 3:7) and gynosaponin addition (0 mg and 16 mg) in a 2×2 double factorial design. In the presence of gynosaponin, methane production and acetate concentration were significantly decreased, whereas concentration of propionate tended to be increased resulting in a significant reduction (p<0.05) of acetate:propionate ratio (A:P ratio), in high-forage substrate. Gynosaponin treatment increased (p<0.05) the butyrate concentration in both F:C ratios. Denaturing gradient gel electrophoresis (DGGE) analysis showed there was no apparent shift in the composition of total bacteria, protozoa and methanogens after treated by gynosaponin under both F:C ratios. The real-time polymerase chain reaction (PCR) analysis indicated that variable F:C ratios significantly affected the abundances of Fibrobacter succinogenes, Rumninococcus flavefaciens, total fungi and counts of protozoa (p<0.05), but did not affect the mcrA gene copies of methanogens and abundance of total bacteria. Counts of protozoa and abundance of F.succinogenes were decreased significantly (p<0.05), whereas mcrA gene copies of methanogens were decreased slightly (p<0.10) in high-forage substrate after treated by gynosaponin. However, gynosaponin treatment under high-concentrate level did not affect the methanogenesis, fermentation characteristics and tested microbes. Accordingly, overall results suggested that gynosaponin supplementation reduced the in vitro methanogenesis and improved rumen fermentation under high-forage condition by changing the abundances of related rumen microbes.

No MeSH data available.


Related in: MedlinePlus

Denaturing gradient gel electrophoresis profile of ruminal bacteria. HF+0 mg = high forage (F:C = 70:30)+0 mg gynosaponin; HF+16 mg = high-forage (F:C = 70:30)+16 mg gynosaponin; HC+0 mg = High-concentrate (F:C = 30:70)+0 mg gynosaponin; HC+16 mg = High concentrate (F:C = 30:70)+16 mg gynosaponin, B1, B2, B3, B4; replications.
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f2-ajas-27-8-1088-4: Denaturing gradient gel electrophoresis profile of ruminal bacteria. HF+0 mg = high forage (F:C = 70:30)+0 mg gynosaponin; HF+16 mg = high-forage (F:C = 70:30)+16 mg gynosaponin; HC+0 mg = High-concentrate (F:C = 30:70)+0 mg gynosaponin; HC+16 mg = High concentrate (F:C = 30:70)+16 mg gynosaponin, B1, B2, B3, B4; replications.

Mentions: Denaturing gradient gel electrophoresis profiles of bacteria, methanogens and protozoa are shown in Figure 2, 3, and 4, respectively. After 48 h fermentation, in addition to some different bands between two kinds of substrates, no special bands appeared for the composition of total bacteria, protozoa and methanogens after treated by gynosaponin in both high and low F:C ratios.


Effect of Gynosaponin on Rumen In vitro Methanogenesis under Different Forage-Concentrate Ratios.

Manatbay B, Cheng Y, Mao S, Zhu W - Asian-australas. J. Anim. Sci. (2014)

Denaturing gradient gel electrophoresis profile of ruminal bacteria. HF+0 mg = high forage (F:C = 70:30)+0 mg gynosaponin; HF+16 mg = high-forage (F:C = 70:30)+16 mg gynosaponin; HC+0 mg = High-concentrate (F:C = 30:70)+0 mg gynosaponin; HC+16 mg = High concentrate (F:C = 30:70)+16 mg gynosaponin, B1, B2, B3, B4; replications.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4109864&req=5

f2-ajas-27-8-1088-4: Denaturing gradient gel electrophoresis profile of ruminal bacteria. HF+0 mg = high forage (F:C = 70:30)+0 mg gynosaponin; HF+16 mg = high-forage (F:C = 70:30)+16 mg gynosaponin; HC+0 mg = High-concentrate (F:C = 30:70)+0 mg gynosaponin; HC+16 mg = High concentrate (F:C = 30:70)+16 mg gynosaponin, B1, B2, B3, B4; replications.
Mentions: Denaturing gradient gel electrophoresis profiles of bacteria, methanogens and protozoa are shown in Figure 2, 3, and 4, respectively. After 48 h fermentation, in addition to some different bands between two kinds of substrates, no special bands appeared for the composition of total bacteria, protozoa and methanogens after treated by gynosaponin in both high and low F:C ratios.

Bottom Line: In the presence of gynosaponin, methane production and acetate concentration were significantly decreased, whereas concentration of propionate tended to be increased resulting in a significant reduction (p<0.05) of acetate:propionate ratio (A:P ratio), in high-forage substrate.However, gynosaponin treatment under high-concentrate level did not affect the methanogenesis, fermentation characteristics and tested microbes.Accordingly, overall results suggested that gynosaponin supplementation reduced the in vitro methanogenesis and improved rumen fermentation under high-forage condition by changing the abundances of related rumen microbes.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Gastrointestinal Microbiology, Nanjing Agricultural University, Nanjing 210095, China.

ABSTRACT
The study aimed to investigate the effects of gynosaponin on in vitro methanogenesis under different forage-concentrate ratios (F:C ratios). Experiment was conducted with two kinds of F:C ratios (F:C = 7:3 and F:C = 3:7) and gynosaponin addition (0 mg and 16 mg) in a 2×2 double factorial design. In the presence of gynosaponin, methane production and acetate concentration were significantly decreased, whereas concentration of propionate tended to be increased resulting in a significant reduction (p<0.05) of acetate:propionate ratio (A:P ratio), in high-forage substrate. Gynosaponin treatment increased (p<0.05) the butyrate concentration in both F:C ratios. Denaturing gradient gel electrophoresis (DGGE) analysis showed there was no apparent shift in the composition of total bacteria, protozoa and methanogens after treated by gynosaponin under both F:C ratios. The real-time polymerase chain reaction (PCR) analysis indicated that variable F:C ratios significantly affected the abundances of Fibrobacter succinogenes, Rumninococcus flavefaciens, total fungi and counts of protozoa (p<0.05), but did not affect the mcrA gene copies of methanogens and abundance of total bacteria. Counts of protozoa and abundance of F.succinogenes were decreased significantly (p<0.05), whereas mcrA gene copies of methanogens were decreased slightly (p<0.10) in high-forage substrate after treated by gynosaponin. However, gynosaponin treatment under high-concentrate level did not affect the methanogenesis, fermentation characteristics and tested microbes. Accordingly, overall results suggested that gynosaponin supplementation reduced the in vitro methanogenesis and improved rumen fermentation under high-forage condition by changing the abundances of related rumen microbes.

No MeSH data available.


Related in: MedlinePlus