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Effect of Gynosaponin on Rumen In vitro Methanogenesis under Different Forage-Concentrate Ratios.

Manatbay B, Cheng Y, Mao S, Zhu W - Asian-australas. J. Anim. Sci. (2014)

Bottom Line: In the presence of gynosaponin, methane production and acetate concentration were significantly decreased, whereas concentration of propionate tended to be increased resulting in a significant reduction (p<0.05) of acetate:propionate ratio (A:P ratio), in high-forage substrate.However, gynosaponin treatment under high-concentrate level did not affect the methanogenesis, fermentation characteristics and tested microbes.Accordingly, overall results suggested that gynosaponin supplementation reduced the in vitro methanogenesis and improved rumen fermentation under high-forage condition by changing the abundances of related rumen microbes.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Gastrointestinal Microbiology, Nanjing Agricultural University, Nanjing 210095, China.

ABSTRACT
The study aimed to investigate the effects of gynosaponin on in vitro methanogenesis under different forage-concentrate ratios (F:C ratios). Experiment was conducted with two kinds of F:C ratios (F:C = 7:3 and F:C = 3:7) and gynosaponin addition (0 mg and 16 mg) in a 2×2 double factorial design. In the presence of gynosaponin, methane production and acetate concentration were significantly decreased, whereas concentration of propionate tended to be increased resulting in a significant reduction (p<0.05) of acetate:propionate ratio (A:P ratio), in high-forage substrate. Gynosaponin treatment increased (p<0.05) the butyrate concentration in both F:C ratios. Denaturing gradient gel electrophoresis (DGGE) analysis showed there was no apparent shift in the composition of total bacteria, protozoa and methanogens after treated by gynosaponin under both F:C ratios. The real-time polymerase chain reaction (PCR) analysis indicated that variable F:C ratios significantly affected the abundances of Fibrobacter succinogenes, Rumninococcus flavefaciens, total fungi and counts of protozoa (p<0.05), but did not affect the mcrA gene copies of methanogens and abundance of total bacteria. Counts of protozoa and abundance of F.succinogenes were decreased significantly (p<0.05), whereas mcrA gene copies of methanogens were decreased slightly (p<0.10) in high-forage substrate after treated by gynosaponin. However, gynosaponin treatment under high-concentrate level did not affect the methanogenesis, fermentation characteristics and tested microbes. Accordingly, overall results suggested that gynosaponin supplementation reduced the in vitro methanogenesis and improved rumen fermentation under high-forage condition by changing the abundances of related rumen microbes.

No MeSH data available.


Related in: MedlinePlus

Curves of cumulative gas production (A) and methane production (B). HF+0 mg = high forage (F:C = 70:30)+0 mg gynosaponin; HF+16 mg = high-forage (F:C = 70:30)+16 mg gynosaponin; HC+0 mg = High-concentrate (F:C = 30:70)+0 mg gynosaponin; HC+16 mg = high concentrate (F:C = 30:70)+16 mg gynosaponin. Values presented are the average of the replicate cultures (n = 4). Error bars represent the standard error of the mean.
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f1-ajas-27-8-1088-4: Curves of cumulative gas production (A) and methane production (B). HF+0 mg = high forage (F:C = 70:30)+0 mg gynosaponin; HF+16 mg = high-forage (F:C = 70:30)+16 mg gynosaponin; HC+0 mg = High-concentrate (F:C = 30:70)+0 mg gynosaponin; HC+16 mg = high concentrate (F:C = 30:70)+16 mg gynosaponin. Values presented are the average of the replicate cultures (n = 4). Error bars represent the standard error of the mean.

Mentions: The cumulative production of total gas and methane during each measuring point are shown in Figure 1A and 1B. The total gas production and methane production showed a similar increasing pattern. However, the total gas production with high-concentrate substrate showed a relatively faster increasing curve than that with high-forage substrate. There was no difference between gyposaponin treatment and the control on cumulative gas production and methane production at each measuring point under high-concentrate substrates. Gynosaponin addition apparently reduced the total gas and methane production from the 4 h of incubation in high-forage substrate. This effect was evident (Table 2) during the 48 h fermentation process, both total gas and methane production of high-concentrate substrate were significantly greater than those of the high-forage substrate. Changes in methane production through gynosaponin treatment were F:C ratio dependent where gynosaponin addition reduced the cumulative methane production by 14.49% (p<0.05) in the high-forage substrate after 48 h fermentation, while no changes were observed in high-concentrate level.


Effect of Gynosaponin on Rumen In vitro Methanogenesis under Different Forage-Concentrate Ratios.

Manatbay B, Cheng Y, Mao S, Zhu W - Asian-australas. J. Anim. Sci. (2014)

Curves of cumulative gas production (A) and methane production (B). HF+0 mg = high forage (F:C = 70:30)+0 mg gynosaponin; HF+16 mg = high-forage (F:C = 70:30)+16 mg gynosaponin; HC+0 mg = High-concentrate (F:C = 30:70)+0 mg gynosaponin; HC+16 mg = high concentrate (F:C = 30:70)+16 mg gynosaponin. Values presented are the average of the replicate cultures (n = 4). Error bars represent the standard error of the mean.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4109864&req=5

f1-ajas-27-8-1088-4: Curves of cumulative gas production (A) and methane production (B). HF+0 mg = high forage (F:C = 70:30)+0 mg gynosaponin; HF+16 mg = high-forage (F:C = 70:30)+16 mg gynosaponin; HC+0 mg = High-concentrate (F:C = 30:70)+0 mg gynosaponin; HC+16 mg = high concentrate (F:C = 30:70)+16 mg gynosaponin. Values presented are the average of the replicate cultures (n = 4). Error bars represent the standard error of the mean.
Mentions: The cumulative production of total gas and methane during each measuring point are shown in Figure 1A and 1B. The total gas production and methane production showed a similar increasing pattern. However, the total gas production with high-concentrate substrate showed a relatively faster increasing curve than that with high-forage substrate. There was no difference between gyposaponin treatment and the control on cumulative gas production and methane production at each measuring point under high-concentrate substrates. Gynosaponin addition apparently reduced the total gas and methane production from the 4 h of incubation in high-forage substrate. This effect was evident (Table 2) during the 48 h fermentation process, both total gas and methane production of high-concentrate substrate were significantly greater than those of the high-forage substrate. Changes in methane production through gynosaponin treatment were F:C ratio dependent where gynosaponin addition reduced the cumulative methane production by 14.49% (p<0.05) in the high-forage substrate after 48 h fermentation, while no changes were observed in high-concentrate level.

Bottom Line: In the presence of gynosaponin, methane production and acetate concentration were significantly decreased, whereas concentration of propionate tended to be increased resulting in a significant reduction (p<0.05) of acetate:propionate ratio (A:P ratio), in high-forage substrate.However, gynosaponin treatment under high-concentrate level did not affect the methanogenesis, fermentation characteristics and tested microbes.Accordingly, overall results suggested that gynosaponin supplementation reduced the in vitro methanogenesis and improved rumen fermentation under high-forage condition by changing the abundances of related rumen microbes.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Gastrointestinal Microbiology, Nanjing Agricultural University, Nanjing 210095, China.

ABSTRACT
The study aimed to investigate the effects of gynosaponin on in vitro methanogenesis under different forage-concentrate ratios (F:C ratios). Experiment was conducted with two kinds of F:C ratios (F:C = 7:3 and F:C = 3:7) and gynosaponin addition (0 mg and 16 mg) in a 2×2 double factorial design. In the presence of gynosaponin, methane production and acetate concentration were significantly decreased, whereas concentration of propionate tended to be increased resulting in a significant reduction (p<0.05) of acetate:propionate ratio (A:P ratio), in high-forage substrate. Gynosaponin treatment increased (p<0.05) the butyrate concentration in both F:C ratios. Denaturing gradient gel electrophoresis (DGGE) analysis showed there was no apparent shift in the composition of total bacteria, protozoa and methanogens after treated by gynosaponin under both F:C ratios. The real-time polymerase chain reaction (PCR) analysis indicated that variable F:C ratios significantly affected the abundances of Fibrobacter succinogenes, Rumninococcus flavefaciens, total fungi and counts of protozoa (p<0.05), but did not affect the mcrA gene copies of methanogens and abundance of total bacteria. Counts of protozoa and abundance of F.succinogenes were decreased significantly (p<0.05), whereas mcrA gene copies of methanogens were decreased slightly (p<0.10) in high-forage substrate after treated by gynosaponin. However, gynosaponin treatment under high-concentrate level did not affect the methanogenesis, fermentation characteristics and tested microbes. Accordingly, overall results suggested that gynosaponin supplementation reduced the in vitro methanogenesis and improved rumen fermentation under high-forage condition by changing the abundances of related rumen microbes.

No MeSH data available.


Related in: MedlinePlus